Analysis of the enhancing effect of HIV restriction factors on cellular immune responses

• Project/Area Number: 15H04737
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Matano Tetsuro 国立感染症研究所, エイズ研究センター, センター長 (00270653)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: ウイルス / 微生物 / 免疫学 / 細胞傷害性Tリンパ球 / HLA / 抗原提示 / 感染症

Outline of Final Research Achievements

The purpose of this study is to elucidate the effect of interaction between viral antigens and host restriction factors such as APOBEC3G on the presentation of viral antigen-derived epitopes to cytotoxic T lymphocytes (CTLs). Our analysis revealed that APOBEC3G-positive cells expressing a CTL epitope linked to human immunodeficiency virus (HIV) Vif antigens are highly sensitive to killing by the epitope-specific CTLs. This novel finding indicates that the interaction between HIV Vif and host APOBEC3G may support HIV replication by suppressing the inhibitory effect of APOBEC3G but enhance sensitivity of HIV-infected cells to CTL killing.

Research Products

• [Int'l Joint Research] Tulane National Primate Research Center/University of Miami School of Medicine/VLP Therapeutics(米国)
• [Int'l Joint Research] Tulane National Primate Research Center/VLP Therapeutics(米国)
• [Journal Article] Characterization of In Vitro Expanded Virus-Specific T cells for Adoptive Immunotherapy against Virus Infection (2018)
  • Author(s): Ono T, Fujita Y, Matano T, Takahashi S, Morio T, Kawana-Tachikawa A
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 71 Issue: 2 Pages: 122-128
  • DOI: 10.7883/yoken.JJID.2017.500
  • NAID: 130006517125
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Open Access
• [Journal Article] In vivo virulence of MHC-adapted AIDS virus serially-passaged through MHC-mismatched hosts (2017)
  • Author(s): Seki Sayuri、Nomura Takushi、Nishizawa Masako、Yamamoto Hiroyuki、Ishii Hiroshi、Matsuoka Saori、Shiino Teiichiro、Sato Hironori、Mizuta Kazuta、Sakawaki Hiromi、Miura Tomoyuki、Naruse Taeko K.、Kimura Akinori、Matano Tetsuro
  • Journal Title: PLOS Pathogens Volume: 13 Issue: 9 Pages: e1006638-e1006638
  • DOI: 10.1371/journal.ppat.1006638
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic HIV-1 genetic recombination and genotypic drug resistance among treatment-experienced adults in northern Ghana (2017)
  • Author(s): Nii-Trebi Nicholas Israel、Brandful James Ashun Mensah、Ibe Shiro、Sugiura Wataru、Barnor Jacob Samson、Bampoh Patrick Owiredu、Yamaoka Shoji、Matano Tetsuro、Yoshimura Kazuhisa、Ishikawa Koichi、Ampofo William Kwabena
  • Journal Title: Journal of Medical Microbiology Volume: 66 Issue: 11 Pages: 1663-1672
  • DOI: 10.1099/jmm.0.000621
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Biphasic CD8+ T-cell defense in elite SIV control by acute-phase passive neutralizing antibody immunization (2016)
  • Author(s): Iseda S, Takahashi N, Poplimont H, Nomura T, Seki S, Nakane T, Nakamura M, Shi S, Ishii H, Furukawa S, Harada S, Naruse TK, Kimura A, Matano T, Yamamoto H
  • Journal Title: J Virol Volume: 90 Issue: 14 Pages: 6276-6290
  • DOI: 10.1128/jvi.00557-16
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Association of lymph-node antigens with lower Gag-specific central-memory and higher Env-specific effector-memory CD8+ T-cell frequencies in a macaque AIDS model (2016)
  • Author(s): Ishii H, Matsuoka S, Nomura T, Nakamura M, Shiino T, Sato Y, Iwata-Yoshikawa N, Hasegawa H, Mizuta K, Sakawaki H, Miura T, Koyanagi Y, Naruse TK, Kimura A, Matano T
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 30153-30153
  • DOI: 10.1038/srep30153
  • NAID: 120006346255
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recursion-based depletion of human immunodeficiency virus-specific naive CD4+ T cells may facilitate persistent viral replication and chronic viraemia leading to acquired immunodeficiency syndrome (2016)
  • Author(s): Tsukamoto T, Yamamoto H, Okada S, Matano T
  • Journal Title: Med Hypotheses Volume: 94 Pages: 81-85
  • DOI: 10.1016/j.mehy.2016.06.024
  • Peer Reviewed
• [Journal Article] Augmentation of anti-SIV activity in CD8+ cells by neutralizing but not non-neutralizing antibodies in the acute phase (2016)
  • Author(s): Yamamoto H, Iseda S, Nakane T, Nomura T, Takahashi N, Seki S, Nakamura M, Ishii H, Matano T
  • Journal Title: AIDS Volume: 30 Pages: 2391-2394
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Patterns of HIV/SIV prevention and control by passive antibody immunization (2016)
  • Author(s): Yamamoto H, Matano T
  • Journal Title: Front Micobiol Volume: 7 Pages: 1739-1739
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of a Sendai virus vector-based AIDS vaccine inducing T cell responses (2016)
  • Author(s): Seki S, Matano T
  • Journal Title: Expert Rev Vaccines Volume: 15 Issue: 1 Pages: 119-127
  • DOI: 10.1586/14760584.2016.1105747
  • Peer Reviewed
• [Journal Article] Broadening of Virus-Specific CD8+ T-Cell Responses Is Indicative of Residual Viral Replication in Aviremic SIV Controllers (2015)
  • Author(s): Takushi Nomura, Hiroyuki Yamamoto, Hiroshi Ishii, Hirofumi Akari, Taeko K. Naruse, Akinori Kimura, Tetsuro Matano
  • Journal Title: PLOS Pathogens Volume: 11 Issue: 11 Pages: e1005247-e1005247
  • DOI: 10.1371/journal.ppat.1005247
  • NAID: 120005763387
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Virus-Host CD8+ T Cell Interaction in HIV/SIV Infection (2017)
  • Author(s): Matano T
  • Organizer: XII Advanced Course on HIV Pathogenesis
  • Int'l Joint Research / Invited
• [Presentation] HIV/SIVと宿主T細胞との相互作用 (2017)
  • Author(s): 俣野哲朗
  • Organizer: 白馬シンポジウム
  • Invited
• [Presentation] Analysis of wild-type and mutant epitope-specific CD8+ T cell responses in SIV controllers (2017)
  • Author(s): omura T, Ishii H, Seki S, Yamamoto H, Terahara K, Miura T, Matano T
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Dynamics of wild-type and mutant epitope-specific CD8+ T-cell responses in SIV infection (2017)
  • Author(s): Matano T
  • Organizer: The 18th Kumamoto AIDS Seminar
  • Int'l Joint Research / Invited
• [Presentation] Development of vaccines using Sendai virus vectors (2017)
  • Author(s): Matano T
  • Organizer: III International Conference on Vaccines Research and Development
  • Int'l Joint Research / Invited
• [Presentation] Analysis of in vivo replication capacity of MHC-I adapted SIV (2017)
  • Author(s): eki S, Nomura T, Nishizawa M, Ishii H, Miura T, Matano T
  • Organizer: 第31回日本エイズ学会学術集会
• [Presentation] サルエイズモデルにおけるウイルス複製の長期制御メカニズム (2016)
  • Author(s): 俣野哲朗
  • Organizer: 第30回日本エイズ学会学術集会
  • Place of Presentation: 鹿児島
  • Year and Date: 2016-11-24
  • Invited
• [Presentation] Virus-host immune interaction in SIV infection (2016)
  • Author(s): Matano T
  • Organizer: The 17th Kumamoto AIDS Seminar
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 2016-11-01
  • Int'l Joint Research / Invited
• [Presentation] Sustained B-cell responses correlate with neutralizing antibody induction in SIVmac239 infection (2016)
  • Author(s): Yamamoto H, Matano T
  • Organizer: The 34th annual symposium on non-human primate models for AIDS
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2016-10-14
  • Int'l Joint Research
• [Presentation] Analysis of proviral genome sequences in multiple cell subsets in SIV-infected rhesus macaques (2016)
  • Author(s): Nomura T, Yamamoto H, Terahara K, Seki S, Matano T
  • Organizer: The 34th annual symposium on non-human primate models for AIDS
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2016-10-12
  • Int'l Joint Research
• [Presentation] Multiple transmissions of SIV adapted to protective MHC alleles in rhesus macaques (2016)
  • Author(s): Seki S, Nomura T, Nishizawa M, Matano T
  • Organizer: The 34th annual symposium on non-human primate models for AIDS
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2016-10-12
  • Int'l Joint Research
• [Presentation] Virus-host T cell interaction in SIV infection (2016)
  • Author(s): Matano T
  • Organizer: AIDS Panel Meeting, U.S.-Japan Cooperative Medical Sciences Program
  • Place of Presentation: Bethesda, MD, USA
  • Year and Date: 2016-01-14
  • Int'l Joint Research / Invited
• [Presentation] HIVワクチン開発研究の進展状況 (2015)
  • Author(s): 俣野哲朗
  • Organizer: 第56回日本熱帯医学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2015-12-05
  • Invited
• [Presentation] Effects of IL-7 and IL-15 administration on SIV controllers (2015)
  • Author(s): Nomura T, Yamamoto H, Matano T
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-24
• [Presentation] Lasting viral control by CD8+ T cells in a macaque AIDS model (2015)
  • Author(s): Matano T
  • Organizer: 13th International Symposium on Molecular Basis of Viral Diseases
  • Place of Presentation: 東京
  • Year and Date: 2015-11-20
  • Invited
• [Presentation] Antigen-specific CD8+ T-cell responses in rhesus macaque lymph nodes in the chronic phase of SIVmac239 infection (2015)
  • Author(s): Matano T
  • Organizer: The 16th Kumamoto AIDS Seminar
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 2015-10-08
  • Int'l Joint Research / Invited