| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
We aimed to clarify the molecular mechanisms of the step by which myeloid-T-B progenitors are committed to myeloid-T progenitors. In 2015, we reported the time course data of this process as a part of FANTOM5 project conducted by RIEKN（Science, 329:1014, 2015）, and also succeeded in developing an on-off induction culture system of multipotent hematopoietic progenitors（Stem Cell Reports, 2015）. In 2016, we reported that T cell progenitors are converted into B lineage cells upon inactivation of polycomb complex（Gene Dev, 2016）. In one of planned subjects, in which we knocked out snai1 and snai2, which had been listed up from FANTOM5 project as candidate genes that play role in early T cell differentiation, we produced T cell specific conditional double knockout mice, but unfortunately they did not show phenotypes. However, we have achieved publications as mentioned above along with the aim of this study, and so, as a total, we think that the results were satisfactory.
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