Development of new preventive methods for food allergy using of the analysis on epigenetics on lymphocytes and intestinal flora microbiome

• Project/Area Number: 15H04783
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Kanazawa University
• Principal Investigator: NAKAMURA Hiroyuki 金沢大学, 医学系, 教授 (30231476)
• Co-Investigator(Kenkyū-buntansha): 辻口 博聖 金沢大学, 医学系, 特任助教 (00723090) ; 神林 康弘 金沢大学, 医学系, 講師 (20345630) ; 福冨 友馬 独立行政法人国立病院機構(相模原病院臨床研究センター), 診断・治療薬開発研究室, 室長 (30463110) ; 八田 耕太郎 順天堂大学, 医学部, 教授 (90337915)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: 食物アレルギー / アレルゲン / エピジェネティクス / 腸内細菌 / Tリンパ球 / テイラーメイド予防法 / DNAメチル化 / 16S rRNA遺伝子 / Ｔリンパ球

Outline of Final Research Achievements

To develop new biomarker for food allergy, we have performed analysis of trascriptome and epigenome of T cell and the strain of intestinal flora and metagenome in infants aged1-3 with fool allergy and its control. In results, more significantly frequent methylation of T cell and less richness and diversity of the strain were seen in the food allergic infants. These results suggest that less richness and diversity of the strain produce the methylation of T cell, subsequently leading to the development of food allergy. The richness and diversity of the strain may be efficient biomarkers for food allergy.

Research Products

• [Journal Article] Phase I study of weekly palliative chemotherapy with low-dose third-line paclitaxel for biliary tract cancer (2017)
  • Author(s): Tajima H, Ohta T, Shinbashi H, Hirose A, Okazaki M, Yamaguchi T, Ohbatake Y, Okamoto K, Nakanuma S, Sakai S, Kinoshita J, Makino I, Nakamura K, Hayashi H, Oyama K, Inokuchi M, Miyashita T, Takamura H, Ninomiya I, Fushida S, Nakamura H.
  • Journal Title: Mol Clin Oncol Volume: 6(5) Issue: 5 Pages: 753-757
  • DOI: 10.3892/mco.2017.1206
  • Peer Reviewed
• [Journal Article] The Association of Autism Spectrum Disorders and Symptoms of Asthma, Allergic Rhinoconjunctivitis and Eczema among Japanese Children Aged 3 - 6 Years (2017)
  • Author(s): Hori D, Tsujiguchi H, Kambayashi Y, Hamagishi T, Kitaoka M, Mitoma J, Asakura H, Suzuki F, Enoch Olando Anyenda, Nguyen Thi Thu Thao, Yamada H, Tamai S, Hayashi K, Hibino Y, Shibata A, Sagara T, Sasahara S, Matsuzaki I, Nakamura H
  • Journal Title: Health Volume: 9(8) Issue: 08 Pages: 1235-1250
  • DOI: 10.4236/health.2017.98089
  • Peer Reviewed / Open Access
• [Journal Article] Preventive Effects of Suvorexant on Delirium: A Randomized Placebo-Controlled Trial. (2017)
  • Author(s): Hatta K, Kishi Y, Wada K, Takeuchi T, Ito S, Kurata A, Murakami K, Sugita M, Usui C, Nakamura H, DELIRIA-J Group
  • Journal Title: J Clin Psychiatry Volume: 78(8) Issue: 8 Pages: e970-e979
  • DOI: 10.4088/jcp.16m11194
  • Peer Reviewed
• [Journal Article] Sex- and age-specific associations of social status and health-related behaviors with health check attendance: findings from the cross-sectional Kanazawa Study. (2017)
  • Author(s): Tsujiguchi H, Hori D, Kambayashi Y, Hamagishi T, Asakura H, Mitoma J, Kitaoka M, Olando AE, Thao NTT, Yamada Y, Hayashi K, Konoshita T, Sagara T, Shibata A, Nakamura H
  • Journal Title: Health Volume: 9 Issue: 09 Pages: 1285-1300
  • DOI: 10.4236/health.2017.99093
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of serum inflammatory cytokine concentrations in familial Mediterranean fever and rheumatoid arthritis patients (2017)
  • Author(s): Koga T、Kawashiri S-y、Migita K、Sato S、Umeda M、Fukui S、Nishino A、Nonaka F、Iwamoto N、Ichinose K、Tamai M、Nakamura H、Origuchi T、Ueki Y、Masumoto J、Agematsu K、Yachie A、Eguchi K、Kawakami A
  • Journal Title: Scandinavian Journal of Rheumatology Volume: - Issue: 4 Pages: 1-3
  • DOI: 10.1080/03009742.2017.1363281
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Relationship between Vitamin Intake and Depressive Symptoms in Elderly Japanese Individuals: Differences with Gender and Body Mass Index (2017)
  • Author(s): Nguyen TTT, Tsujiguchi H, Kambayashi Y, Hara A, Miyagi S, Yamada Y, Nakamura H, Shimizu Y, Hori D, Suzuki F, Hayashi K, Nakamura H
  • Journal Title: Nutrients Volume: 9(12) Issue: 12 Pages: 1319-1319
  • DOI: 10.3390/nu9121319
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence of primary aldosteronism without hypertension in the general population: Results in Shika study (2017)
  • Author(s): Karashima S, Kometani M, Tsujiguchi H, Asakura H, Nakano S, Usukura M, Mori S, Ohe M, Sawamura T, Okuda R, Hara A, Takamura T, Yamagishi M, Nakamura H, Takeda Y, Yoneda T
  • Journal Title: Clin Exp Hypertens Volume: 40(2) Issue: 2 Pages: 118-125
  • DOI: 10.1080/10641963.2017.1339072
  • Peer Reviewed
• [Journal Article] Time-series analysis: variation of anti-acetylcholine receptor antibody titer in myasthenia gravis is related to incidence of Mycoplasma pneumoniae and influenza virus infections (2017)
  • Author(s): Iwasa K, Yoshikawa H, Hamaguchi T, Sakai K, Shinohara-Noguchi M, Samuraki M, Takahashi K, Yanase D, Ono K, Ishida C, Yoshita M, Nakamura H, Yamada M.
  • Journal Title: Neurological Research Volume: 40 Issue: 2 Pages: 102-109
  • DOI: 10.1080/01616412.2017.1407021
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Combined Analysis of IFN-γ, IL-2, IL-5, IL-10, IL-1RA and MCP-1 in QFT Supernatant Is Useful for Distinguishing Active Tuberculosis from Latent Infection. (2016)
  • Author(s): Suzukawa M, Akashi S, Nagai H, Nagase H, Nakamura H, Matsui H, Hebisawa A, Ohta K
  • Journal Title: PLoS One Volume: 11(4) Issue: 4 Pages: e0152483-e0152483
  • DOI: 10.1371/journal.pone.0152483
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Association between Activity of Daily Living and the Combination of Alzheimer’s Disease and Cataract in Elderly Requiring Nursing Care. (2016)
  • Author(s): Hamagishi T, Inagawa T, Kambayashi Y, Tsujiguchi H, Kitaoka M, Mitoma J, Asakura H, Suzuki F, Hori D, Enoch Olando Anyenda, Nguyen Thi Thu Thao, Hibino Y, Hayashi K, Shibata A, Sagara T, Okochi J, Takamoku K, Hatta K, Konoshita T, Nakamura H
  • Journal Title: Health Volume: 8(10) Issue: 10 Pages: 994-1003
  • DOI: 10.4236/health.2016.810103
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The relationship between hypertension and health-related quality of life: adjusted by chronic pain, chronic diseases, and life habits in the general middle-aged population in Japan. (2016)
  • Author(s): Kitaoka M, Mitoma J, Asakura H, Anyenda EO, Nguyen TT, Hamagishi T, Hori D, Suzuki F, Shibata A, Horii M, Tsujiguchi H, Hibino Y, Kambayashi Y, Hitomi Y, Shikura N, Nakamura H
  • Journal Title: Environ Health Prev Med Volume: 21(4) Issue: 4 Pages: 193-214
  • DOI: 10.1007/s12199-016-0514-6
  • NAID: 210000000436
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Associations of Cough Prevalence with Ambient Polycyclic Aromatic Hydrocarbons, Nitrogen and Sulphur Dioxide: A Longitudinal Study. (2016)
  • Author(s): Anyenda EO, Higashi T, Kambayashi Y, Nguyen TT, Michigami Y, Fujimura M, Hara J, Tsujiguchi H, Kitaoka M, Asakura H, Hori D, Yamada Y, Hayashi K, Hayakawa K, Nakamura H
  • Journal Title: Int J Environ Res Public Health Volume: 13(8) Issue: 8 Pages: 800-805
  • DOI: 10.3390/ijerph13080800
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The associations between lifestyles and mental health using the General Health Questionnaire 12-items are different dependently on age and sex: a population-based cross-sectional study in Kanazawa, Japan. (2016)
  • Author(s): Hori D, Tsujiguchi H, Kambayashi Y, Hamagishi T, Kitaoka M, Mitoma J, Asakura H, Suzuki F, Anyenda EO, Nguyen TT, Hibino Y, Shibata A, Hayashi K, Sagara T, Sasahara S, Matsuzaki I, Hatta K, Konoshita T, Nakamura H
  • Journal Title: Environ Health Prev Med Volume: 21(6) Issue: 6 Pages: 410-421
  • DOI: 10.1007/s12199-016-0541-3
  • NAID: 50010159080
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Usefulness of Electrocardiographic Voltage to Determine Myocardial Fibrosis in Hypertrophic Cardiomyopathy. (2016)
  • Author(s): Konno T, Nagata Y, Teramoto R, Fujino N, Nomura A, Tada H, Sakata K, Furusho H, Takamura M, Nakamura H, Kawashiri MA, Yamagishi M, Hayashi K.
  • Journal Title: Am J Cardiol. Volume: 117 Issue: 3 Pages: 443-449
  • DOI: 10.1016/j.amjcard.2015.11.015
  • Peer Reviewed / Open Access
• [Journal Article] A Longitudinal Study of Association between Heavy Metals and Itchy Eyes, Coughing in Chronic Cough Patients: Related with Non-Immunoglobulin E Mediated Mechanism (2016)
  • Author(s): Thao Thi Thu Nguyen , Tomomi Higashi , Yasuhiro Kambayashi , Enoch Olando Anyenda , Yoshimasa Michigami , Johsuke Hara , Masaki Fujimura , Hiromasa Tsujiguchi , Masami Kitaoka , Hiroki Asakura , Daisuke Hori , Yuri Hibino, Tadashi Konoshita and Hiroyuki Nakamura
  • Journal Title: Int. J. Environ. Res. Public Health Volume: 13 Issue: 1 Pages: 110-123
  • DOI: 10.3390/ijerph13010110
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prevalence of Chronic Pain, Especially Headache, and Relationship with Health-Related Quality of Life in Middle-Aged Japanese Residents. (2016)
  • Author(s): Mitoma J, Kitaoka M, Asakura H, Anyenda EO, Hori D, Nguyen TT, Hamagishi T, Hayashi K, Suzuki F, Shimizu Y, Tsujiguchi H, Kambayashi Y, Hibino Y, Konoshita T, Sagara T, Shibata A, Nakamura H
  • Journal Title: Health Volume: 8 Issue: 01 Pages: 116-124
  • DOI: 10.4236/health.2016.81014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pharmacokinetics, safety and tolerability of Melissa officinalis extract which contained rosmarinic acid in healthy individuals: A randomized controlled trial. (2015)
  • Author(s): Noguchi-Shinohara M., Ono K., Hamaguchi T., Iwasa K., Nagai T., Kobayashi S., Nakamura H., Yamada M.
  • Journal Title: PLoS One Volume: 10 Issue: 5 Pages: e0126422-e0126422
  • DOI: 10.1371/journal.pone.0126422
  • Peer Reviewed / Open Access
• [Journal Article] Right ventricular hypertrophy is associated with cardiovascular events in hypertrophic cardiomyopathy: evidence from study with magnetic resonance imaging. (2015)
  • Author(s): Nagata Y, Konno T, Fujino N, Hodatsu A, Nomura A, Hayashi K, Nakamura H, Kawashiri MA, Yamagishi M.
  • Journal Title: Can J Cardiol. Volume: 31 Issue: 6 Pages: 702-708
  • DOI: 10.1016/j.cjca.2014.12.036
  • Peer Reviewed / Open Access
• [Journal Article] Semi-comprehensive analysis of gene amplification in gastric cancers using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization. (2015)
  • Author(s): Ooi A, Oyama T, Nakamura R, Tajiri R, Ikeda H, Fushida Y, Nakamura H, Dobashi Y.
  • Journal Title: Modern Pathology Volume: in press Issue: 6 Pages: 861-871
  • DOI: 10.1038/modpathol.2015.33
  • NAID: 120005593968
  • Peer Reviewed
• [Journal Article] A Genetic Variant in the Distal Enhancer Region of the Human Renin Gene Affects Renin Expression. (2015)
  • Author(s): Makino Y, Konoshita T, Omori A, Maegawa N, Nakaya T, Ichikawa M, Yamamoto K, Wakahara S, Ishizuka T, Onoe T, Nakamura H; Genomic Disease Outcome Consortium (G-DOC) Study Investigators
  • Journal Title: PLoS One. Volume: 10(9) Issue: 9 Pages: e0137469-e0137469
  • DOI: 10.1371/journal.pone.0137469
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] シンポジウム「生活用品中のパラベン類によるアレルギー性疾患発症に関する総合的研究」 (2018)
  • Author(s): 福富友馬，中村裕之，山本貴和子，中村剛，弘田量二
  • Organizer: 第88回日本衛生学会
• [Presentation] 気道炎症指標を含めた喘息重症度別クラスター解析 (2016)
  • Author(s): 長瀬洋之、釣木澤尚実、岩永賢司、田中明彦、谷田貝洋平、斎藤純平、鈴川真穂、東元一晃、井上博雅、棟方充、檜澤伸之、相良博典、東田有智、秋山一男、出原賢治、中村裕之、大田健
  • Organizer: 第65回日本アレルギー学会学術大会
  • Place of Presentation: 東京国際フォーラム（東京）
  • Year and Date: 2016-06-17
• [Presentation] 管理栄養士養成課程における歯科口腔保健分野の教育課程について (2016)
  • Author(s): 柴田亜樹、三浦千佳、林宏一、逸見眞理子、大西孝司、木戸康人、西村優希、中村裕之
  • Organizer: 第86回日本衛生学会学術総会
  • Place of Presentation: 旭川市民文化会館（北海道）
  • Year and Date: 2016-05-11
• [Presentation] 黄砂を含む環境中化学物質の呼吸器疾患への影響に対するゼロ次予防 (2015)
  • Author(s): 中村裕之、神林康弘、東朋美
  • Organizer: 第13回日本予防医学会学術総会
  • Place of Presentation: フレンドパーク石川（石川県）
  • Year and Date: 2015-06-20
• [Presentation] 生活習慣および精神的健康度と健診受診行動との関連 (2015)
  • Author(s): 辻口博聖、堀大介、神林康弘、朝倉大貴、北岡政美、三苫純子、Anyenda Enoch Olando、Nguyen Thi Thu Thao、濱岸利夫、中村剛、木戸康人、能登裕幸、鈴木史彦、荒舩丈一、清水由香里、林宏一郎、日比野由利、柴田亜樹、相良多喜子、中村裕之
  • Organizer: 第13回日本予防医学会学術総会
  • Place of Presentation: フレンドパーク石川（石川県）
  • Year and Date: 2015-06-20
• [Presentation] 大気粒子状物質中PAH やPM2.5 やSO2と成人慢性咳嗽患者における咳症状との関連 (2015)
  • Author(s): Anyenda Enoch Olando、神林康弘、東朋美、Nguyen Thi Thu Thao、道上義正、原丈介、藤村政樹、辻口博聖、北岡政美、朝倉大貴、堀大介、三苫純子、中村剛、木戸康人、能登裕幸、日比野由利、中村裕之
  • Organizer: 第13回日本予防医学会学術総会
  • Place of Presentation: フレンドパーク石川（石川県）
  • Year and Date: 2015-06-20
• [Presentation] 慢性咳嗽患者における重金属と目のかゆみと咳との関連 IgE レベルによる違い (2015)
  • Author(s): Nguyen Thi Thu Thao、神林康弘、東朋美、Anyenda Enoch Olando、道上義正、原丈介、藤村政樹、辻口博聖、北岡政美、朝倉大貴、堀大介、三苫純子、中村剛、木戸康人、能登裕幸、日比野由利、中村裕之
  • Organizer: 第13回日本予防医学会学術総会
  • Place of Presentation: フレンドパーク石川（石川県）
  • Year and Date: 2015-06-20
• [Presentation] 精神的健康度と性，年代，生活習慣要因の関連－金沢市一般住民の横断調査 (2015)
  • Author(s): 堀大介、辻口博聖、神林康弘、濱岸利夫、北岡政美、三苫純子、朝倉大貴、鈴木史彦、Anyenda Enoch Olando、Nguyen Thi Thu Thao、中村 剛、木戸康人、能登裕幸、荒舩丈一、清水由香里、林宏一郎、日比野由利、柴田亜樹、相良多喜子、笹原信一朗、松崎一葉、中村裕之
  • Organizer: 第13回日本予防医学会学術総会
  • Place of Presentation: フレンドパーク石川（石川県）
  • Year and Date: 2015-06-20
• [Presentation] 地域における歯科医療受診者への栄養・食生活支援に関する研究 (2015)
  • Author(s): 三浦千佳、木戸康人、大滝直人、西村優希、林宏一、柴田亜樹、逸見眞理子、大西孝司、中村裕之
  • Organizer: 第13回日本予防医学会学術総会
  • Place of Presentation: フレンドパーク石川（石川県）
  • Year and Date: 2015-06-20
• [Presentation] 管理栄養士養成における歯科口腔保健分野教育の課題 - 管理栄養士養成課程学生調査結果からみた一考察 - (2015)
  • Author(s): 柴田亜樹、三浦千佳、木戸康人、大滝直人、西村優希、林宏一、逸見眞理子、大西孝司、中村裕之
  • Organizer: 第13回日本予防医学会学術総会
  • Place of Presentation: フレンドパーク石川（石川県）
  • Year and Date: 2015-06-20
• [Presentation] 成人喘息のクラスター解析の現状と展望－厚生労働科学研究（大田班）の知見を含めて－ (2015)
  • Author(s): 長瀬洋之、釣木澤尚実、岩永賢司、田中明彦、谷田貝洋平、斎藤純平、鈴川真穂、東元一晃、井上博雅、棟方充、檜澤伸之、東田有智、秋山一男、出原賢治、中村裕之、大田健
  • Organizer: 第64回日本アレルギー学会学術大会
  • Place of Presentation: グランドプリンスホテル新高輪 国際館パミール（東京）
  • Year and Date: 2015-05-26