Elucidation of the pathology and treatment of refractory small intestinal ulcer disease using genetically engineered mouse and PG mass spectrometry
| Project/Area Number |
15H04811
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
杉浦 悠毅 慶應義塾大学, 医学部(信濃町), 特任講師 (30590202)
金井 隆典 慶應義塾大学, 医学部(信濃町), 教授 (40245478)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 小腸潰瘍 / プロスタグランジン / SLCO2A1 / CEAS / 遺伝性小腸潰瘍症 / 質量分析 / マウスモデル |
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| Outline of Final Research Achievements |
For the diagnosis of a patient with a CEAS patient, a simple diagnostic technique by SLCO 2A1 protein fluorescent immunostaining method was established. For functional analysis of SLCO2A1 mutant protein found in patients, a gene transfer system by Xenopus oocytes was set up and as a result of the transport experiment of PG, Mutations that completely lost function and mutations that only partially declined in function were observed. Furthermore, it was shown that female sex hormone influences PG transporting ability. We established vascular endothelial specific SLCO2A1 deficient mice and established a mass spectrometry method of PG metabolism analysis system in mouse small intestine. As a result, changes in PG metabolism in each small intestinal ulcer model including genetically modified mice could be observed.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Clinical features of chronic enteropathy associated with SLCO2A1 gene: a new entity clinically distinct from Crohn's disease.2018
Author(s)
Umeno J, Esaki M, Hirano A, Fuyuno Y, Ohmiya N, Yasukawa S, Hirai F, Kochi S, Kurahara K, Yanai S, Uchida K, Hosomi S, Watanabe K, Hosoe N, Ogata H, Hisamatsu T, Nagayama M, Yamamoto H, Abukawa D, Kakuta F, Onodera K, Matsui T, Hibi T, Yao T, Kitazono T, Matsumoto T;CEAS study group.
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Journal Title
J Gastroenterol
Volume: Jan 8
Issue: 8
Pages: 907-915
DOI
Related Report
Peer Reviewed
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[Journal Article] A Hereditary Enteropathy Caused by Mutations in the SLCO2A1 Gene, Encoding a Prostaglandin Transporter.2015
Author(s)
Umeno J, Hisamatsu T, Esaki M, Atsushi Hirano A, Kubokura N, Asano K, Kochi S, Yanai S, Fuyuno Y, Shimamura K, Hosoe N, Ogata H, Watanabe T, Aoyagi K, Ooi H, Watanabe K, Yasukawa S, Hirai F, Matsui T, Iida M, Yao T, Hibi T, Kosaki K, Kanai T, Kitazono T, Matsumoto T.
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Journal Title
PLoS Genetics
Volume: Nov 5;11(11):
Issue: 11
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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