| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
For the diagnosis of a patient with a CEAS patient, a simple diagnostic technique by SLCO 2A1 protein fluorescent immunostaining method was established. For functional analysis of SLCO2A1 mutant protein found in patients, a gene transfer system by Xenopus oocytes was set up and as a result of the transport experiment of PG, Mutations that completely lost function and mutations that only partially declined in function were observed. Furthermore, it was shown that female sex hormone influences PG transporting ability. We established vascular endothelial specific SLCO2A1 deficient mice and established a mass spectrometry method of PG metabolism analysis system in mouse small intestine. As a result, changes in PG metabolism in each small intestinal ulcer model including genetically modified mice could be observed.
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