Role of regulatory plasmablasts pReg in colitis

• Project/Area Number: 15H04813
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kurume University
• Principal Investigator: MIZOGUCHI Atsushi 久留米大学, 医学部, 教授 (50258472)
• Co-Investigator(Kenkyū-buntansha): 小松 誠和 久留米大学, 医学部, 講師 (50343687)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: 制御性B細胞Breg / 制御性形質芽細胞Preg / 炎症性腸疾患 / マウスモデル / インターロイキンー10 / 制御性形質芽細胞 / インターロイキン10 / 潰瘍性大腸炎 / Ｐreg / 免疫学 / 内科 / 応用動物 / 細胞・組織

Outline of Final Research Achievements

Due to the specific ability of B cells to produce immunoglobulins, B cells were generally believed to contribute always for immune activation. However, IL-10-producing B cells with immune suppressive activity were discovered in 2002, and it is becoming apparent increasingly that IL-10-producing B cells are associated with a wide variety of diseases. In this project, we unexpectedly found that IL-10-producing cells, which were believed as B cells due to the expression pattern of surface markers, rather represent plasmablasts. The IL-10-producing plasmablasts have an ability to produce IgA, exist in the spleen of mice, and expand in mesenteric lymph nodes under an intestinal inflammatory condition. The expanded IL-10-producing plasmablasts then contribute for the improvement of colitis. These findings unveil an unexpected function of plasmablasts in intestinal inflammation.

Research Products

• [Int'l Joint Research] Massachusetts General Hospital(米国)
• [Journal Article] Glycaemic control boosts glucosylated nanocarrier crossing the BBB into the brain (2017)
  • Author(s): Anraku Y.、Kuwahara H.、Fukusato Y.、Mizoguchi A.、Ishii T.、Nitta K.、Matsumoto Y.、Toh K.、Miyata K.、Uchida S.、Nishina K.、Osada K.、Itaka K.、Nishiyama N.、Mizusawa H.、Yamasoba T.、Yokota T.、Kataoka K.
  • Journal Title: Nat Commun Volume: 8 Issue: 1 Pages: 2001-2009
  • DOI: 10.1038/s41467-017-00952-3
  • Peer Reviewed / Open Access
• [Journal Article] Interleukin-10-producing B cells in autoimmune bullous diseases (2017)
  • Author(s): Hashimoto T, Mizoguchi A
  • Journal Title: Br J Dermatol Volume: 176 Issue: 5 Pages: 1119-1120
  • DOI: 10.1111/bjd.15513
• [Journal Article] Current understanding of dysbiosis in disease in human and animal models. (2016)
  • Author(s): DeGruttola AK, Low D, Mizoguchi A, and Mizoguchi E.
  • Journal Title: Ｉｎｆｌａｍｍａｔｏｒｙ Ｂｏｗｅｌ Ｄｉｓｅａｓｅ Volume: 225 Issue: 5 Pages: 1136-1150
  • DOI: 10.1097/mib.0000000000000750
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetically engineered mouse models for studying Inflammatory Bowel Disease (2016)
  • Author(s): Mizoguchi A, Takeuchi T, Himuro H, Okada T, Mizoguchi E.
  • Journal Title: J Pathol Volume: 238 Issue: 2 Pages: 205-219
  • DOI: 10.1002/path.4640
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Protective role of IL-10 producing plasmablasts (Preg) in colitis (2018)
  • Author(s): Yano A, Nishida A, Andoh A, Mizoguchi E, Mizoguchi A
  • Organizer: Falk Symposium
  • Int'l Joint Research
• [Presentation] IBDから見るB細胞のなぞ (2017)
  • Author(s): 溝口充志
  • Organizer: 日本消化器免疫学会
  • Invited
• [Presentation] T-bet dependent IL-12-producing B cells for the termination of inflammatory responses (2017)
  • Author(s): Yano A, Nishida A, Mizoguchi E, Andoh A, Mizoguchi A
  • Organizer: GI Research Academy
  • Int'l Joint Research
• [Presentation] T-bet-dependent IL-12-producing regulatory B cells Breg in the intestine (2016)
  • Author(s): Yano A, Nishida A, Takeuchi T, Mizoguchi E, and Mizoguchi A
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 沖縄
  • Year and Date: 2016-12-05
• [Presentation] Colonic IL-10 producing plasmalasts in UC patients (2016)
  • Author(s): Takeuchi T, Nishida A, Kizaki J, Kinugasa T, Akagi Y, Noake T, Araki Y, Andoh A, Mitsuyama K, Mizoguchi A
  • Organizer: The 4th Annual Meeting of Asian Organization of Crohn`s and Colitis
  • Place of Presentation: 京都
  • Year and Date: 2016-07-07
  • Int'l Joint Research
• [Remarks] Ｂｒｅｇ
  • URL: http://www.med.kurume-u.ac.jp/med/immun/mizoguchi.html
• [Remarks] 久留米大学医学部免疫学講座ホームページ
  • URL: http://www.med.kurume-u.ac.jp/med/immun/mizoguchi.html