Trial to develop a personalized adoptive immunotherapy

• Project/Area Number: 15H04831
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Hiroshima University
• Principal Investigator: Hattori Noboru 広島大学, 医歯薬保健学研究科(医), 教授 (00283169)
• Co-Investigator(Kenkyū-buntansha): 河本 宏 京都大学, ウイルス・再生医科学研究所, 教授 (00343228)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 養子免疫療法 / 癌特異的変異抗原 / 腫瘍浸潤性Tリンパ球 / 癌細胞培養 / スフェロイド培養 / がん免疫療法 / 癌免疫療法 / iPS細胞 / 細胞株樹立 / 癌組織浸潤リンパ球

Outline of Final Research Achievements

To develop a personalized immunotherapy of lung cancer, we searched for a new tumor antigen specific to tumor cells. We collected peripheral blood mononuclear cells and malignant pleural effusion, and established primary cultures of cancer cells derived from pleural effusion. Whole-exome sequencing of mononuclear cells and cancer cells derived from the same patient identified 389 indels and 6616 SNPs. We found several candidates for new tumor antigens using the computational algorithm to identify HLA-binding peptides.

Research Products

• [Journal Article] Aminopeptidase N/CD13 as a Potential Therapeutic Target in Malignant Pleural Mesothelioma (2018)
  • Author(s): Otsuki Takahiko、Nakashima Taku、Hamada Hironobu、Takayama Yusuke、Akita Shin、Masuda Takeshi、Horimasu Yasushi、Miyamoto Shintaro、Iwamoto Hiroshi、Fujitaka Kazunori、Miyata Yoshihiro、Miyake Masayuki、Kohno Nobuoki、Okada Morihito、Hattori Noboru
  • Journal Title: European Respiratory Journal Volume: 51 Issue: 5 Pages: 1701610-1701610
  • DOI: 10.1183/13993003.01610-2017
  • Peer Reviewed
• [Journal Article] The extent of ground-glass attenuation is a risk factor of chemotherapy-related exacerbation of interstitial lung disease in patients with non-small cell lung cancer (2017)
  • Author(s): Masuda Takeshi, Hirano Chihiro, Horimasu Yasushi, Nakashima Taku, Miyamoto Shintarou, Iwamoto Hiroshi, Ohshimo Shinichiro, Fujitaka Kazunori, Hamada Hironobu, Hattori Noboru
  • Journal Title: Cancer Chemotherapy and Pharmacology Volume: 81 Issue: 1 Pages: 131-139
  • DOI: 10.1007/s00280-017-3476-5
  • Peer Reviewed
• [Journal Article] Additional Octreotide Therapy to Sirolimus Achieved a Decrease in Sirolimus-refractory Chylous Effusion Complicated with Lymphangioleiomyomatosis (2017)
  • Author(s): Namba M, Masuda T, Nakamura T, Horimasu Y, Miyamoto S, Nakashima T, Iwamoto H, Fujitaka K, Hamada H, Hattori N.
  • Journal Title: Internal Medicine Volume: 56 Issue: 24 Pages: 3327-3331
  • DOI: 10.2169/internalmedicine.8645-16
  • NAID: 130006250509
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] Chronic Intestinal Pseudo-obstruction and Orthostatic Hypotension Associated with Small Cell Lung Cancer that Improved with Tumor Reduction after Chemoradiotherapy (2017)
  • Author(s): Izumi Y, Masuda T, Horimasu Y, Nakashima T, Miyamoto S, Iwamoto H, Fujitaka K, Hamada H, Hattori N.
  • Journal Title: Internal Medicine Volume: 56 Issue: 19 Pages: 2627-2631
  • DOI: 10.2169/internalmedicine.8574-16
  • NAID: 130006110679
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] AGER rs2070600 polymorphism elevates neutrophil-lymphocyte ratio and mortality in metastatic lung adenocarcinoma (2017)
  • Author(s): Yamaguchi K, Iwamoto H, Ohshimo S, et al.
  • Journal Title: Oncotarget Volume: 8 Issue: 55 Pages: 94382-94392
  • DOI: 10.18632/oncotarget.21764
  • Peer Reviewed / Open Access
• [Journal Article] Feasibility Study of Personalized Peptide Vaccination for Advanced Small Cell Lung Cancer. (2017)
  • Author(s): Sakamoto S, Yamada T, Terazaki Y, Yoshiyama K, Sugawara S, Takamori S, Matsueda S, Shichijo S, Yamada A, Noguchi M, Itoh K, Hattori N, Kohno N, Sasada T.
  • Journal Title: Clin Lung Cancer. Volume: 印刷中 Issue: 6 Pages: e385-e394
  • DOI: 10.1016/j.cllc.2017.03.011
  • Peer Reviewed / Open Access
• [Journal Article] MUC1 in lung adenocarcinoma: cross-sectional genetic and serological study (2017)
  • Author(s): Horimasu Yasushi、Ishikawa Nobuhisa、Tanaka Sonosuke、Hirano Chihiro、Iwamoto Hiroshi、Ohshimo Shinichiro、Fujitaka Kazunori、Hamada Hironobu、Hattori Noboru、Kohno Nobuoki
  • Journal Title: BMC Cancer Volume: 17 Issue: 1 Pages: 263-263
  • DOI: 10.1186/s12885-017-3272-y
  • NAID: 120006559419
  • Peer Reviewed / Open Access
• [Journal Article] Immunological evaluation of peptide vaccination for cancer patients with the HLA -A11+ or -A33+ allele. (2017)
  • Author(s): Sakamoto S, Matsueda S, Takamori S, Toh U, Noguchi M, Yutani S, Yamada A, Shichijo S, Yamada T, Suekane S, Kawano K, Naitou M, Sasada T, Hattori N, Kohno N, Itoh K.
  • Journal Title: Cancer Sci. Volume: 印刷中 Issue: 4 Pages: 598-603
  • DOI: 10.1111/cas.13189
  • Peer Reviewed / Open Access
• [Journal Article] MT95-4, a fully humanized antibody raised against aminopeptidase N, reduces tumor progression in a mouse model. (2015)
  • Author(s): Akita S, Hattori N, Masuda T, Horimasu Y, Nakashima T, Iwamoto H, Fujitaka K, Miyake M, Kohno N
  • Journal Title: Cancer science Volume: 106 Issue: 7 Pages: 921-928
  • DOI: 10.1111/cas.12692
  • Peer Reviewed / Open Access
• [Journal Article] Immunological evaluation of peptide vaccination for cancer patients with the HLA-A26 allele. (2015)
  • Author(s): Sakamoto S, Matsueda S, Takamori S, Toh U, Noguchi M, Yutani S, Yamada A, Shichijo S, Yamada T, Suekane S, Kawano K, Sasada T, Hattori N, Kohno N, Itoh K.
  • Journal Title: Cancer Sci. Volume: 106 Issue: 10 Pages: 1257-1263
  • DOI: 10.1111/cas.12757
  • NAID: 120006799352
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of prognostic significance of granulocyte-related factors in cancer patients undergoing personalized peptide vaccination. (2015)
  • Author(s): Sakamoto S, et al.
  • Journal Title: Hum Vaccin Immunother Volume: 11 Pages: 2784-2789
  • Peer Reviewed
• [Journal Article] Histological Quantification of Gene Silencing by Intratracheal Administration of Dry Powdered Small-Interfering RNA/Chitosan Complexes in the Murine Lung. (2015)
  • Author(s): Ihara D, Hattori N, Horimasu Y, Masuda T, Nakashima T, Senoo T, Iwamoto H, Fujitaka K, Okamoto H, Kohno N
  • Journal Title: Pharmaceutical research Volume: 32 Issue: 12 Pages: 3877-3885
  • DOI: 10.1007/s11095-015-1747-6
  • Peer Reviewed / Open Access