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A study on the MUC4 gene that is a candidate of the cause of the diffuse alveolar damage observed in Japanese

Research Project

Project/Area Number 15H04832
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionJichi Medical University

Principal Investigator

Hagiwara Koichi  自治医科大学, 医学部, 教授 (00240705)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords薬剤性肺障害 / びまん性肺胞障害 / 上皮成長因子受容体 / 上皮成長因子受容体阻害薬 / 日本人 / チロシンキナーゼ / MUC4 / 日本人特異的 / ERBB2 / ERBB3 / 次世代シークエンサー / 遺伝学 / 医療・福祉 / 遺伝子 / 分子標的薬 / EGFR阻害薬 / 肺障害 / 気道上皮
Outline of Final Research Achievements

The determination of the frequency of MUC4 polymorphic alleles in multiple ethnic groups, and the construction of the expression vectors are steadily in progress. The procedures that are required for assembling the nucleotide sequences from the output of the next generation sequencers are determined, and the assembler is made using the Ruby programming language. As for the MUC4 expression vectors, 4 different polymorphic sequences were used and the FLAG tag has been added to the C-terminus. Introduction of the vector into COS7 cells successfully expressed the gene in about 20% of the cells. The detailed investigation of the signaling pathway is underway.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (6 results)

All 2017 2016

All Journal Article (3 results) Presentation (3 results)

  • [Journal Article] 肺循環・肺損傷 ムチンと気道上皮防御機構2017

    • Author(s)
      太田洋充、萩原弘一
    • Journal Title

      分子呼吸器病

      Volume: 21 Pages: 61-63

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 炎症・線維化 間質性肺炎マーカー2016

    • Author(s)
      坂東政司、萩原弘一
    • Journal Title

      生体の科学

      Volume: 67 Pages: 448-449

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 薬剤性肺障害、特発性肺線維症急性増悪の遺伝的素因に関する検索2016

    • Author(s)
      萩原弘一
    • Journal Title

      呼吸と循環

      Volume: 64 Pages: 144-148

    • Related Report
      2016 Annual Research Report
  • [Presentation] TKI関連ILDの感受性遺伝子2017

    • Author(s)
      萩原弘一
    • Organizer
      日本肺癌学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 当院の進展型肺小細胞癌における間質性肺炎の有無の影響および予後因子の検討2016

    • Author(s)
      5.岩井悠希、渡辺恭孝、工藤史明、三輪千尋、長井良昭、太田洋充、小山信一郎、萩原弘一、小山信之
    • Organizer
      第57回日本肺癌学会学術集会
    • Place of Presentation
      福岡(福岡サンパレス、福岡国際センター)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高速シークエンサーによる包括的遺伝子変異検索(MINtS)を用いた肺癌手術症例の解析2016

    • Author(s)
      3.井上慶明、杉山亜斗、青木耕平、福田佑樹、儀賀理暁、泉陽太、小林国彦、中山光男、萩原弘一
    • Organizer
      第75回日本癌学会総会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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