| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
The determination of the frequency of MUC4 polymorphic alleles in multiple ethnic groups, and the construction of the expression vectors are steadily in progress. The procedures that are required for assembling the nucleotide sequences from the output of the next generation sequencers are determined, and the assembler is made using the Ruby programming language. As for the MUC4 expression vectors, 4 different polymorphic sequences were used and the FLAG tag has been added to the C-terminus. Introduction of the vector into COS7 cells successfully expressed the gene in about 20% of the cells. The detailed investigation of the signaling pathway is underway.
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