Elucidation of molecular architecture of neuromuscular junction and dissection of molecular pathomechanisms of congenital myasthenic syndromes

• Project/Area Number: 15H04840
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Nagoya University
• Principal Investigator: Ohno Kinji 名古屋大学, 医学系研究科, 教授 (80397455)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 先天性筋無力症候群 / アセチルコリン受容体 / Rspo2 / GFPT1 / iPS細胞 / 神経筋接合部 / RSPO2 / DOK7 / LGR5 / agrin

Outline of Final Research Achievements

Laser capture microdissection of the mouse spinal motor neurons (SMN) revealed that Rspo2 is highly expressed in spinal motor neurons. Rspo2 induces acetylcholine receptor (AChR) clustering, which is ~80% as potent as agrin. Tissue-specific rescue of Rspo2 by transgenic expression of Rspo2 by SMN- and muscle-specific promoters revealed that SMN-derived Rspo2 plays essential roles in the formation of the neuromuscular junction and AChR clustering. We propose that Rspo2 is an essential AChR clustering-inducing molecule after agrin.
We started dissection of molecular pathomechanisms of mutations in a gene encoding glycosylation enzyme, GFPT1, which were identified in Japanese patients with congenital myasthenic syndrome.

Research Products

• [Journal Article] MYRF is associated with encephalopathy with reversible myelin vacuolization (2018)
  • Author(s): Kurahashi Hirokazu、Azuma Yoshiteru、Masuda Akio、Okuno Tatsuya、Nakahara Eri、Imamura Takuji、Saitoh Makiko、Mizuguchi Masashi、Shimizu Toshiaki、Ohno Kinji、Okumura Akihisa
  • Journal Title: Annals of Neurology Volume: 83 Issue: 1 Pages: 98-106
  • DOI: 10.1002/ana.25125
  • Peer Reviewed / Open Access
• [Journal Article] Lack of Fgf18 causes abnormal clustering of motor nerve terminals at the neuromuscular junction with reduced acetylcholine receptor clusters (2018)
  • Author(s): Ito Kenyu、Ohkawara Bisei、Yagi Hideki、Nakashima Hiroaki、Tsushima Mikito、Ota Kyotaro、Konishi Hiroyuki、Masuda Akio、Imagama Shiro、Kiyama Hiroshi、Ishiguro Naoki、Ohno Kinji
  • Journal Title: Sceintific Reports Volume: 8 Issue: 1 Pages: 434-434
  • DOI: 10.1038/s41598-017-18753-5
  • Peer Reviewed / Open Access
• [Journal Article] Molecular hydrogen upregulates heat shock response and collagen biosynthesis, and downregulates cell cycles - Meta-analyses of gene expression profiles (2018)
  • Author(s): Hiroshi Nishiwak,i, Ito Mikako , Shuto Negishi, Sobue Sayaka, Ichihara Masatoshi, Ohno Kinji
  • Journal Title: Free Radic Res. Volume: - Issue: 4 Pages: 1-12
  • DOI: 10.1080/10715762.2018.1439166
  • Peer Reviewed
• [Journal Article] Loss of Sfpq causes long-gene transcriptopathy in the brain (2018)
  • Author(s): Takeuchi A, Iida K, Tsubota T, Hosokawa M, Denawa M, Ninomiya K, Ito M, Kimura H, Abe T, Kiyonari H, Ohno K, Hagiwara M.
  • Journal Title: Cell Rep Volume: -
  • NAID: 120006463742
  • Peer Reviewed
• [Journal Article] Rules and tools to predict the splicing effects of exonic and intronic mutations (2018)
  • Author(s): Ohno K, Takeda J, Masuda A.
  • Journal Title: Wiley Interdiscip Rev RNA Volume: 印刷中 Issue: 1
  • DOI: 10.1002/wrna.1451
  • NAID: 120006473502
  • Peer Reviewed
• [Journal Article] Maternal administration of meclozine for the treatment of foramen magnum stenosis in transgenic mice with achondroplasia (2017)
  • Author(s): Matsushita M, Mishima K, Esaki R, Ishiguro N, Ohno K, Kitoh H
  • Journal Title: J Neurosurg Pediatr Volume: 19 Issue: 1 Pages: 91-95
  • DOI: 10.3171/2016.7.peds16199
  • Peer Reviewed / Open Access
• [Journal Article] Protein-anchoring therapy of biglycan for mdx mouse model of Duchenne muscular dystrophy (2017)
  • Author(s): Ito M*, Ehara Y*, Li J, Inada K, Ohno K. *Equal contribution.
  • Journal Title: Hum Gene Ther Volume: - Issue: 5 Pages: 428-436
  • DOI: 10.1089/hum.2015.088
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Six GU-rich (6GUR) FUS-binding motifs detected by normalization of CLIP-seq by Nascent-seq (2017)
  • Author(s): Takeda J, Masuda A, Ohno K.
  • Journal Title: Gene Volume: 618 Pages: 57-64
  • DOI: 10.1016/j.gene.2017.04.008
  • NAID: 120006331826
  • Peer Reviewed / Open Access
• [Journal Article] Wnt/β-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells (2017)
  • Author(s): Kishimoto Yasuzumi、Ohkawara Bisei、Sakai Tadahiro、Ito Mikako、Masuda Akio、Ishiguro Naoki、Shukunami Chisa、Docheva Denitsa、Ohno Kinji
  • Journal Title: PLoS One Volume: 12 Issue: 7 Pages: e0182051-e0182051
  • DOI: 10.1371/journal.pone.0182051
  • Peer Reviewed / Open Access
• [Journal Article] SRSF1 suppresses selection of intron-distal 5′ splice site of DOK7 intron 4 to generate functional full-length Dok-7 protein (2017)
  • Author(s): Ahsan Khalid Bin、Masuda Akio、Rahman Mohammad Alinoor、Takeda Jun-ichi、Nazim Mohammad、Ohkawara Bisei、Ito Mikako、Ohno Kinji
  • Journal Title: Sceintific Reports Volume: 7 Issue: 1 Pages: 10446-10446
  • DOI: 10.1038/s41598-017-11036-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An ENU-induced splice site mutation of mouse Col1a1 causing recessive osteogenesis imperfecta and revealing a novel splicing rescue. (2017)
  • Author(s): Tabeta K, Du X, Arimatsu K, Yokoji M, Takahashi N, Amizuka N, Hasegawa T, Crozat K, Maekawa T, Miyauchi S, Matsuda Y, Ida T, Kaku M, Hoebe K, Ohno K, Yoshie H, Yamazaki K, Moresco EMY, Beutler B
  • Journal Title: Sci. Rep. Volume: 7(1) Issue: 1 Pages: 11717-11717
  • DOI: 10.1038/s41598-017-10343-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fluoxetine ameliorates cartilage degradation in osteoarthritis by inhibiting Wnt/β-catenin signaling (2017)
  • Author(s): Miyamoto Kentaro、Ohkawara Bisei、Ito Mikako、Masuda Akio、Hirakawa Akihiro、Sakai Tadahiro、Hiraiwa Hideki、Hamada Takashi、Ishiguro Naoki、Ohno Kinji
  • Journal Title: PLoS One Volume: 12 Issue: 9 Pages: e0184388-e0184388
  • DOI: 10.1371/journal.pone.0184388
  • Peer Reviewed / Open Access
• [Journal Article] Progression of Parkinson's disease is associated with gut dysbiosis: Two-year follow-up study (2017)
  • Author(s): Minato Tomomi、Maeda Tetsuya、Fujisawa Yoshiro、Tsuji Hirokazu、Nomoto Koji、Ohno Kinji、Hirayama Masaaki
  • Journal Title: PLOS ONE Volume: 12 Issue: 11 Pages: e0187307-e0187307
  • DOI: 10.1371/journal.pone.0187307
  • Peer Reviewed
• [Journal Article] Promethazine hydrochloride inhibits ectopic fat cell formation in skeletal musce. (2017)
  • Author(s): Kasai T, Tsuchida, K. et al.,
  • Journal Title: Am. J. Pathol. Volume: 187 Issue: 12 Pages: 2627-2634
  • DOI: 10.1016/j.ajpath.2017.08.008
  • Peer Reviewed / Open Access
• [Journal Article] Splicing regulation and dysregulation of cholinergic genes expressed at the neuromuscular junction (2017)
  • Author(s): Ohno, K. Rahman, M. A. Nazim, M. Nasrin, F. Lin, Y. Takeda, J. I. Masuda, A.
  • Journal Title: J Neurochem Volume: 印刷中 Issue: S2 Pages: 64-72
  • DOI: 10.1111/jnc.13954
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Agrin-LRP4-MuSK signaling as a therapeutic target for myasthenia gravis and other neuromuscular disorders (2017)
  • Author(s): Ohno Kinji、Ohkawara Bisei、Ito Mikako
  • Journal Title: Expert Opin Ther Targets. Volume: 21 Issue: 10 Pages: 949-958
  • DOI: 10.1080/14728222.2017.1369960
  • Peer Reviewed
• [Journal Article] Competitive regulation of alternative splicing and alternative polyadenylation by hnRNP H and CstF64 determines acetylcholinesterase isoforms (2017)
  • Author(s): Nazim M, Masuda A, Rahman MA, Nasrin F, Takeda JI, Ohe K, Ohkawara B, Ito M, Ohno K.
  • Journal Title: Nucleic Acids Res Volume: - Pages: 1455-1468
  • DOI: 10.1093/nar/gkw823
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phenylbutazone induces expression of MBNL1 and suppresses formation of MBNL1-CUG RNA foci in a mouse model of myotonic dystrophy (2016)
  • Author(s): 1.Chen G, Masuda A, Konishi H, Ohkawara B, Ito M, Kinoshita M, Kiyama H, Matsuura T, Ohno K.
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 25317-25317
  • DOI: 10.1038/srep25317
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5. (2016)
  • Author(s): Nakashima H., Ohkawara B., Ishigaki S., Fukudome T., Ito K., Tsushima M., Konishi H., Okuno T., Yoshimura T., Ito M., Masuda A., Sobue G., Kiyama H., Ishiguro N., Ohno K.
  • Journal Title: Sci Rep Volume: 22 Issue: 1 Pages: 28512-28512
  • DOI: 10.1038/srep28512
  • Peer Reviewed / Open Access
• [Journal Article] Global identification of hnRNP A1 binding sites for SSO-based splicing modulation (2016)
  • Author(s): Bruun GH, Doktor TK, Borch J-J, Masuda A, Krainer AR, Ohno K, Andresen BS.
  • Journal Title: BMC Biol Volume: 14 Issue: 1 Pages: 54-54
  • DOI: 10.1186/s12915-016-0279-9
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular hydrogen suppresses activated Wnt-catenin signaling. (2016)
  • Author(s): Y. Lin, B. Ohkawara, M. Ito, N. Misawa, K. Miyamoto, Y. Takegami, A. Masuda, S. Toyokuni, K Ohno
  • Journal Title: Scientific Report Volume: 25 Issue: 1 Pages: 31986-31986
  • DOI: 10.1038/srep31986
  • Peer Reviewed / Open Access
• [Journal Article] Mutations causing slow-channel myasthenia reveal that a valine ring in the channel pore of muscle AChR is optimized for stabilizing channel gating (2016)
  • Author(s): Shen X-M*, Okuno T*, Milone M, Otsuka K, Takahashi K, Komaki H, Giles E, Ohno K, Engel AG. *Equal contribution.
  • Journal Title: Hum Mutat Volume: 37 Issue: 10 Pages: 1051-1059
  • DOI: 10.1002/humu.23043
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Is the serum creatine kinase level elevated in congenital myasthenic syndrome? (2016)
  • Author(s): Ohno K.
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: 87 (8) Issue: 8 Pages: 801-801
  • DOI: 10.1136/jnnp-2016-313416
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recent advances in congenital myasthenic syndromes (2016)
  • Author(s): Ohno K, Ohkawara B, Ito M.
  • Journal Title: Clin Exp Neuroimmunol Volume: 7 Issue: 3 Pages: 246-259
  • DOI: 10.1111/cen3.12316
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] FUS-mediated regulation of alternative RNA processing in neurons: insights from global transcriptome analysis (2016)
  • Author(s): Akio Masuda. Takeda, J. I. Ohno, K.
  • Journal Title: Wiley Interdiscip Rev RNA Volume: in press Issue: 3 Pages: 330-340
  • DOI: 10.1002/wrna.1338
  • NAID: 120005893268
  • Peer Reviewed / Open Access
• [Journal Article] IntSplice: Prediction of the splicing consequences of intronic single nucleotide variations in the human genome (2016)
  • Author(s): Shibata A, Okuno T, Rahman MA, Azuma Y, Takeda J, Masuda A, Ohno K.
  • Journal Title: J Hum Genet Volume: in press
  • NAID: 120005851030
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Position-specific binding of FUS to nascent RNA regulates mRNA length. (2015)
  • Author(s): Masuda A, Takeda J, Okuno T, Okamoto T, Ohkawara B, Ito M, Ishigaki S, Sobue G, Ohno K.
  • Journal Title: Genes Dev. Volume: 29(10) Issue: 10 Pages: 1045-57
  • DOI: 10.1101/gad.255737.114
  • Peer Reviewed / Open Access
• [Journal Article] Impaired Synaptic Development, Maintenance, and Neuromuscular Transmission in LRP4-Related Myasthenia. (2015)
  • Author(s): Selcen D, Ohkawara B, Shen XM, McEvoy K, Ohno K, Engel AG.
  • Journal Title: JAMA Neurol. Volume: 72(8) Issue: 8 Pages: 889-96
  • DOI: 10.1001/jamaneurol.2015.0853
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A missense mutation in domain III in HSPG2 in Schwartz-Jampel syndrome compromises secretion of perlecan into the extracellular space. (2015)
  • Author(s): Iwata S, Ito M, Nakata T, Noguchi Y, Okuno T, Ohkawara B, Masuda A, Goto T, Adachi M, Osaka H, Nonaka R, Arikawa-Hirasawa E, Ohno K.
  • Journal Title: Neuromuscul Disord. Volume: 25(8) Issue: 8 Pages: 667-71
  • DOI: 10.1016/j.nmd.2015.05.002
  • Peer Reviewed
• [Journal Article] SRSF1 and hnRNP H antagonistically regulate splicing of COLQ exon 16 in a congenital myasthenic syndrome. (2015)
  • Author(s): Rahman MA, Azuma Y, Nasrin F, Takeda J, Nazim M, Bin Ahsan K, Akio Masuda, Engel AG, Ohno K.
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 13208-13208
  • DOI: 10.1038/srep13208
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Collagen Q and anti-MuSK autoantibody competitively suppress agrin/LRP4/MuSK signaling. (2015)
  • Author(s): Otsuka K, Ito M, Ohkawara B, Masuda A, Kawakami Y, Sahashi K, Nishida H, Mabuchi N, Takano A, Engel AG, Ohno K.
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 13928-13928
  • DOI: 10.1038/srep13928
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Splicing aberrations in congenital myasthenic syndromes (2015)
  • Author(s): Rahman MA, Ohno K.
  • Journal Title: J Investig Genomics Volume: 2 Issue: 5 Pages: 38-38
  • DOI: 10.15406/jig.2015.02.00038
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] LRP4 third β-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner (2014)
  • Author(s): Ohkawara B, Cabrera-Serrano M, Nakata T, Milone M, Asai N, Ito K, Ito M, Masuda A, Ito Y, Engel AG, Ohno K
  • Journal Title: Hum Mol Genet. Volume: Apr 1;23(7) Issue: 7 Pages: 1856-68
  • DOI: 10.1093/hmg/ddt578
  • Peer Reviewed / Open Access
• [Presentation] Clinically Feasible Dose Of Meclozine Promotes Bone Growth In Mouse Model With Achondroplasia (2017)
  • Author(s): Matsushita M, Kitoh H, Mishima K, Sugiura H, Kitamura A, Ishiguro N, Ohno K
  • Organizer: ORS 2017 Annual Meeting
  • Int'l Joint Research
• [Presentation] Fluoxetine ameliorates cartilage degradation in osteoarthritis by inhibiting Wnt/β-catenin signaling (2017)
  • Author(s): Miyamoto K, Ohkawara B, Sakai T, Hiraiwa H, Hamada T, Ohno K, Ishiguro N
  • Organizer: ORS 2017 Annual Meeting
  • Int'l Joint Research
• [Presentation] Japanese myotonic dystrophy type 2 patients carry a haplotype different from the European founder haplotype (2017)
  • Author(s): Matsuura T, Kimura T, Kitao R, Komori T, Toda T, Ohno K.
  • Organizer: 11th International myotonic dystrophy consortium meeting (IDMC-11)
  • Int'l Joint Research
• [Presentation] Competitive regulation of alternative splicing and alternative polyadenylation by hnRNP H and CstF64 determines acetylcholinesterase isoforms (2017)
  • Author(s): Nazim M, Masuda A, Rahman MA, Nasrin F, Takeda J, Ohe K, Ohkawara B, Ito M, Ohno K.
  • Organizer: 2017 CSHL Meeting: Eukaryotic mRNA Processing
  • Int'l Joint Research
• [Presentation] SRSF1 suppresses selection of intron-distal 5’ splice site of DOK7 intron 4 to generate functional full-length Dok-7 protein (2017)
  • Author(s): Ahsan KB, Masuda A, Rahman MA, Takeda J, Ohkawara B, Ito M, Ohno K.
  • Organizer: 2017 CSHL Meeting: Eukaryotic mRNA Processing
  • Int'l Joint Research
• [Presentation] Splicing regulation of the human acetylcholinesterase gene (2016)
  • Author(s): Ohno K, Nazim M, Masuda A.
  • Organizer: XVth International Symposium on Cholinergic Mechanisms
  • Place of Presentation: Marseille, France
  • Year and Date: 2016-10-16
  • Int'l Joint Research / Invited
• [Presentation] スプライシングシス因子の破綻によるスプライシング異常の分子機構とその予測ツール (2015)
  • Author(s): 大野欽司
  • Organizer: 第38回分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸市中央区)
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] LRP4 Myasthenia. Investigation of second kinship reveals impaired development and maintenance of the neuromuscular junction (2015)
  • Author(s): Selcen D, Ohkawara B, Shen X-M, McEvoy K, Ohno K, Engel AG
  • Organizer: WMS Congress [Neuromuscular Disorders 25: ( Suppl 2) : S186-S187]
  • Place of Presentation: Brighton (UK)
  • Year and Date: 2015-09-30
  • Int'l Joint Research
• [Presentation] Roles of collagen Q in MuSK antibody‐positive myasthenia gravis (2015)
  • Author(s): Ohno K
  • Organizer: 12th International Meeting of Cholinesterases
  • Place of Presentation: Alicante (Spain)
  • Year and Date: 2015-09-27
  • Int'l Joint Research / Invited
• [Presentation] 筋チャネル病‐周期性四肢麻痺および非ジストロフィー性ミオトニー症候群の遺伝子解析と麻痺発作重症度分類 (2015)
  • Author(s): 高橋正紀、佐々木良元、久保田智哉、穀内洋介、古田充、中森雅之、望月秀樹、冨本秀和、大野欽司
  • Organizer: 第1回日本筋学会学術集会
  • Place of Presentation: 国立精神・神経医療研究センター（東京都小平市）
  • Year and Date: 2015-08-07
• [Presentation] Schwartz-Jampel 症候群を引き起こすHSPG2 (パールカン)の遺伝子変異 (2015)
  • Author(s): 伊藤美佳子、岩田哲、中田智彦、野口 陽一朗、奥野 達矢、大河原美静、増田章男、後藤知英、安達昌功、小坂 仁、野中里紗、平澤恵理、大野欽司
  • Organizer: 第1回日本筋学会学術集会
  • Place of Presentation: 国立精神・神経医療研究センター（東京都小平市）
  • Year and Date: 2015-08-07
• [Presentation] Physiology and hereditary/autoimmune pathology of acetylcholine receptor clustering at the neuromuscular junction (2015)
  • Author(s): Ohno K
  • Organizer: 10th Japanese-French Workshop“New advances in treatments of neuromuscular diseases: From Basic to Applied Myology”
  • Place of Presentation: Paris (France)
  • Year and Date: 2015-07-02
  • Int'l Joint Research / Invited
• [Book] 「先天性筋無力症候群はどのような病気ですか」in『筋疾患』西野一三監修 神経内科Clinical Question and Pearls (2018)
  • Author(s): 大野欽司
  • Publisher: 中外医学社
• [Book] 「先天性筋無力症候群の治療研究」in 『筋肉研究の最前線』武田伸一監修 CLINICAL CALCIUM 27(3): 97-104, 2017 (2017)
  • Author(s): 大野欽司
  • Total Pages: 144
  • Publisher: 医薬ジャーナル社
  • ISBN: 9784753286232
• [Book] 「ドラッグリポジション戦略による神経筋骨格系病態治療（第28階日本末梢神経学会学術集会教育研修講演1）」in末梢神経 28(2) (2017)
  • Author(s): 大野欽司、大河原美静
  • Total Pages: 400
  • Publisher: 日本末梢神経学会事務局
• [Book] "A hereditary mutation in Schwartz-Jampel syndrome " in Atlas of Science Ed. by Lynn C Yeoman. http://atlasofscience.org/a-hereditary-mutation-in-schwartz-jampel-syndrome/ (2015)
  • Author(s): Ito M, Ohno K.
  • Publisher: AoS Nordic AB, Stockholm
• [Patent(Industrial Property Rights)] Ｒ-スポンジン２によるＷｎｔ／β-カテニンシグナル活性化の制御を介した治療薬 (2018)
  • Inventor(s): 大野欽司、石黒直樹、大河原美静、大倉俊昭
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-050199
  • Filing Date: 2018
• [Patent(Industrial Property Rights)] パーキンソン病の判定マーカーおよび判定方法 (2017)
  • Inventor(s): 平山正昭、大野欽司、辻浩和、朝原崇、野本康二
  • Industrial Property Rights Holder: 国立大学法人名古屋大学、ヤクルト本社
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-098973
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] タンパク質-ＲＮＡ相互作用の検出 (2017)
  • Inventor(s): 増田章男、大野欽司
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-137406
  • Filing Date: 2017