Visualization of cell membrane water permeability for in vivo MRI
| Project/Area Number |
15H04910
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
Obata Takayuki 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 分子イメージング診断治療研究部, 次長(定常) (00285107)
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| Research Collaborator |
Aoki Ichio
Ikoma Yoko
Kawaguchi Hiroshi
Takuwa Hiroyuki
Tachibana Yasuhiko
Yasui Masato
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Keywords | MRI / 細胞膜水透過性 / 水拡散 / アクアポリン / 放射線医学 / 放射線 |
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| Outline of Final Research Achievements |
We aimed to apply the measurement method of cell membrane water permeability established in experiments in vitro to in vivo experiments in rodents. Based on the evidences we also tried to apply it to human MRI.
Additional in vitro experiments were conducted to strengthen the evidence for in vivo applications. A in-vivo-like phantom experiment was performed to adjust the in vivo MRI protocol. In order to quantitatively assess the effects of microcirculation that do not exist in in vitro experiments, the effects were investigated using a unilateral chronic low reflux model mouse. Based on the results of basic research, we obtained feasible results about cell membrane permeability in human using our proposal method.
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| Academic Significance and Societal Importance of the Research Achievements |
腫瘍の悪性度や治療効果で大きく変わるといわれている細胞膜水透過性であるが、これは摘出標本で測定することが困難で、生体そのままでの測定が必要となる。細胞レベルでの測定報告は多いものの、In vivo応用されたケースは極めて少なく、ヒトへの応用性のあるものは皆無といってよい状況であった。そのような中で、本手法は広く臨床で応用されているMRIを用いた非侵襲的な測定技術で、実現できれば新たなバイオマーカーとして有益な生体情報を提供できる可能性がある。
本研究で得られたIn vitro・In vivoの結果から近い将来の臨床応用の可能性を示す複数のエビデンスが得られたことは大きな意義があると考えられる。
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Report
(5 results)
Research Products
(14 results)
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[Presentation] Diffusion-weighted imaging with multiple diffusion time to assess water-exchange between restricted and hindered diffusion components in vivo.2016
Author(s)
Yasuhiko Tachibana1,2, Takayuki Obata1,2, Hiroki Tsuchiya1, Tokuhiko Omatsu1,2, Riwa Kishimoto1,2, Thorsten Feiweier3, and Hiroshi Tsuji1
Organizer
ISMRM 24th Annual Meeting & Exhibitio
Place of Presentation
Suntec Singapore, Singapore
Year and Date
2016-05-07
Related Report
Int'l Joint Research
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