| Project/Area Number |
15H04929
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平松 良浩 浜松医科大学, 医学部, 特任准教授 (00397390)
神谷 欣志 浜松医科大学, 医学部附属病院, 講師 (20324361)
菊池 寛利 浜松医科大学, 医学部附属病院, 講師 (70397389)
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| Research Collaborator |
Ozaki Yusuke 浜松医科大学, 医学部附属病院, 診療助教
Hirotsu Amane 浜松医科大学, 大学院生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 循環腫瘍細胞 / 非染色 / 蛍光位相顕微鏡 / 消化器癌 / 血中循環腫瘍細胞 / 転移 / 定量位相顕微鏡 / 機械学習 / 癌 / 細胞・組織 |
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| Outline of Final Research Achievements |
Circulating tumor cells (CTCs) are used as a liquid biopsy target in cancer patients. In this study, we sought to develop a novel CTC detection method using quantitative phase microscopy (QPM) and computer vision technologies for pattern recognition based on the morphological features of live cells. We observed white blood cells (WBCs) and cancer cell line cells by QPM without staining, and successfully differentiated WBCs from cell lines. We applied our image recognition methods to the cells flowing in the chamber, and properly differentiated WBCs from cell line cells flowing in the chamber. Finally, cell fractions suspicious of CTCs were detected in blood samples of tumor bearing mice and cancer patients.
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