The role of extracellular vesicles in development of metastasis of gastrointestinal cancer

• Project/Area Number: 15H04934
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Otsuji Eigo 京都府立医科大学, 医学(系)研究科(研究院), 教授 (20244600)
• Co-Investigator(Kenkyū-buntansha): 市川 大輔 山梨大学, 大学院総合研究部, 教授 (20347446) ; 塩崎 敦 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40568086) ; 小松 周平 京都府立医科大学, 医学(系)研究科(研究院), 特任講師 (40578978) ; 小西 博貴 京都府立医科大学, 医学部附属病院, 研究員 (00448739) ; 有田 智洋 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00756794)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000); Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: exosome / 胃癌 / 腹膜播種 / 腹水 / ファイブロネクチン-1 / ラミニンガンマ-1 / エクソソーム / 胃十二指腸外科学 / microRNA

Outline of Final Research Achievements

Tumor-derived exosome (TEX) were internalized in both mesothelial and gastric cancer cells in a cellular origin non-specific manner. Expression of adhesion- related molecules, such as fibronectin 1 (FN1) and laminin gamma 1 (LAMC1), were increased in mesothelial cells after internalization of TEX from gastric cancer cell line and malignant pleural effusion. Immunohistochemistry demonstrated higher expression of FN1 and LAMC1 in the disseminated peritoneal membrane than in the benign peritoneal membrane.

Research Products

• [Journal Article] Tumor exosome-mediated promotion of adhesion to mesothelial cells in gastric cancer cells (2016)
  • Author(s): Tomohito Arita, Daisuke Ichikawa, Hirotaka Konishi, Shuhei Komatsu, Atsushi Shiozaki, Shinpei Ogino, Yuji Fujita, Hidekazu Hiramoto, Junichi Hamada, Katsutoshi Shoda, Toshiyuki Kosuga, Hitoshi Fujiwara, Kazuma Okamoto, Eigo Otsuji
  • Journal Title: Oncotarget Volume: 7(35) Issue: 35 Pages: 56855-56863
  • DOI: 10.18632/oncotarget.10869
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 血液、腹水中のExtracellular Vesiclesの胃癌腹膜播種に対する機能解析と新たな治療戦略 (2017)
  • Author(s): 有田智洋
  • Organizer: 第28回日本消化器癌発生学会
  • Invited
• [Presentation] 赤血球由来microvesiclesと胃癌及び大腸癌細胞の悪性化の関連について (2017)
  • Author(s): 有田智洋
  • Organizer: 日本癌治療学会
• [Presentation] 血液中、腹水中の細胞外小胞体 (extracellular vesicles) の消化器癌腹膜播種に対する機能解明と新たな治療への試み (2017)
  • Author(s): 有田智洋
  • Organizer: 日本消化器関連学会週間（JDDW）
• [Presentation] The effect of Erythrocyte-derived microvesicles on the malignant potential of gastric and colorectal cancer (2017)
  • Author(s): 松原大樹・有田智洋
  • Organizer: International Society of Extracellular Vesicles 2017 in Toronto
  • Int'l Joint Research
• [Presentation] Exogenous Factors in Development of Peritoneal Metastasis of Gastric Cancer (2016)
  • Author(s): Tomohiro Arita, Daisuke Ichikawa, Hirotaka Konishi, Katsutoshi Shoda, Junichi Hamada, Hidekazu Hitamoto, Yuji Fujita, Shinpei Ogino, Toshiyuki Kosuga, Shuhei Komatsu, Atsushi Shiozaki, Hitoshi Fujiwara, Kazuma Okamoto, Eigo Otsuji
  • Organizer: 第27回日本消化器癌発生学会
  • Place of Presentation: 鹿児島県、城山観光ホテル
  • Year and Date: 2016-09-15
• [Presentation] 術中出血や腹水exosome・血球由来ectosomeが胃癌腹膜播種に与える影響について (2016)
  • Author(s): 有田智洋 市川大輔 小西博貴 庄田勝俊 濵田隼一 荻野真平 小菅敏幸 小松周平 塩﨑敦 藤原斉 岡本和真 大辻英吾
  • Organizer: 第71回日本消化器外科学会
  • Place of Presentation: 徳島県、あわぎんホール
  • Year and Date: 2016-07-14