• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Transplantation studies of tissue engineered tracha and lung regenerated from recellularized xenogeneic-decellularized scaffold

Research Project

Project/Area Number 15H04944
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory surgery
Research InstitutionNagasaki University

Principal Investigator

TSUCHIYA Tomoshi  長崎大学, 医歯薬学総合研究科(医学系), 准教授 (30437884)

Co-Investigator(Kenkyū-buntansha) 小林 英司  慶應義塾大学, 医学部(信濃町), 特任教授 (00245044)
李 桃生  長崎大学, 原爆後障害医療研究所, 教授 (50379997)
永安 武  長崎大学, 医歯薬学総合研究科(医学系), 教授 (80284686)
樋上 賀一  東京理科大学, 薬学部生命創薬科学科, 教授 (90253640)
秋田 定伯  福岡大学, 医学部, 教授 (90315250)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywords肺再生 / 気管再生 / 脱細胞化 / 臓器移植 / 異種移植 / 組織骨格 / 移植免疫 / 肺移植 / 気管移植 / 再生医療 / 細胞外マトリックス / 組織工学 / 移植 / 再生 / 再細胞化 / 移植・再生医療 / 肺 / 気管 / マトリックス / ラット / ブタ
Outline of Final Research Achievements

We have two major achievements in the present study. ① In the animal decellularized lung scaffold, α-gal epitope, which induce hyper acute reaction in human, was decreased. In addition, α-gal epitope disappeared after the recellularization with human cells. The result suggest transplantation of engineered lung using xeno-decellualrized scaffold will cause little immunoreaction in the human body. ② After the endothelial cell and adipose derived stromal cell (ASC) seeding in the decellularized scaffold, ASC differentiate into pericyte and suppress hemorrhage after the engineered lung transplantation. ASC might play role for vascular maturation. The other hand, xenotransplantation between rat and guinea pig was technically quite difficult.

Academic Significance and Societal Importance of the Research Achievements

この研究の最終形態は、同種や異種の脱細胞化組織骨格を土台とした、自己の再生肺の創出である。臨床応用できるようになれば、現在の移植医療で不足する臓器を一気に解消でき、また、理論的には免疫抑制剤も不要になるため、移植再生医療の革新的な治療法になると考えられる。気管においては、この技術によって、自己の細胞を持つ気管や気管支を、より安価でより簡便に作成可能となる。この手法による再生研究は米国で先行しているが、今後の臓器再生の主流になることも予想される。また細胞接着や幹細胞技術の様々な研究分野に関連しており、この研究を進めること自体が、臓器再生について深い知見を得ることに繋がると考える。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (31 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (22 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Yale大学(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Yale大学/生体工学教室(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 105.Sodium hydroxide based non-detergent decellularizing solution for rat lung.2018

    • Author(s)
      Sengyoku H, Tsuchiya T, Obata T, Doi R, Hashimoto Y, Ishii M, Sakai H, Matsuo N, Taniguchi D, Suematsu T, Lawn M, Matsumoto K, Miyazaki T, Nagayasu T
    • Journal Title

      Organogenesis

      Volume: 11 Issue: 2 Pages: 1-13

    • DOI

      10.1080/15476278.2018.1462432

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adipose-derived mesenchymal stem cells attenuate rejection in a rat lung transplantation model2018

    • Author(s)
      Watanabe H, Tsuchiya T, Shimoyama K,Shimizu S,Akita S,Yukawa H, Baba Y, Nagayasu T
    • Journal Title

      Journal of Surgical Research

      Volume: 227 Pages: 17-27

    • DOI

      10.1016/j.jss.2018.01.016

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transplantation of bioengineered rat lungs recellularized with endothelial and adipose-derived stromal cells2017

    • Author(s)
      Doi Ryoichiro, Tsuchiya Tomoshi, Mitsutake Norisato, Nishimura Satoshi, Matsuu-Matsuyama Mutsumi, Nakazawa Yuka, Ogi Tomoo, Akita Sadanori, Yukawa Hiroshi, Baba Yoshinobu, Yamasaki Naoya, Matsumoto Keitaro, Miyazaki Takuro, Kamohara Ryotaro, Hatachi Go, Sengyoku Hideyori et al.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-15

    • DOI

      10.1038/s41598-017-09115-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 異種脱細胞化気管移植モデルにおける脂肪由来間葉系細胞の免疫抑制効果の検討2019

    • Author(s)
      久永 真
    • Organizer
      第21回日本異種移植研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 自己脂肪由来間葉系幹細胞による免疫抑制効果を目的とした、ラット肺移植モデルにおける細胞治療の試み2019

    • Author(s)
      土谷 智史
    • Organizer
      第119回日本外科学会定期学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞置換技術を利用した 肺再生 -細胞外マトリックスに関わる研究-2019

    • Author(s)
      土谷智史
    • Organizer
      第36回日本呼吸器外科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The transition of the extracellular matrix and alpha-gal antigens in the rat lung scaffold reseeded using human vascular and adipogenic stromal cells2018

    • Author(s)
      橋本 泰匡
    • Organizer
      第20回日本異種移植研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 3次元構築を保った肺再生の取り組みとヒト肺創出への道2018

    • Author(s)
      土谷 智史
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Comparing the Effects of Adipose-derived Mesenchymal Stem Cells Derived from Donor and Recipient in Rat Lung Transplantation Mode2018

    • Author(s)
      下山 孝一郎
    • Organizer
      第41回日本基礎老化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 異種脱細胞骨格を用いた気管移植における脂肪幹細胞の有用性2018

    • Author(s)
      久永 真
    • Organizer
      第54回日本移植学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Potential of Adipose-Derived Stem/Stromal Cells for Lung Transplantation and Lung Tissue Engineering2018

    • Author(s)
      Tomoshi Tsuchiya
    • Organizer
      American Thoracic Society 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Remodeling the vasculature in the decellularized lung scaffold using adipogenic stromal cells2018

    • Author(s)
      Tomoshi Tsuchiya
    • Organizer
      5th TERMIS World Congress 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The transition of extracellular matrix and alpha-gal antigen of rat lung scaffold reseeded by human vascular and adipogenic stromal cells2018

    • Author(s)
      Yasumasa Hashimoto
    • Organizer
      5th TERMIS World Congress 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 異種脱細胞骨格を用いた気管移植における脂肪由来間葉系細胞の有用性2018

    • Author(s)
      久永 真
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Application of decellularized organ scaffolds for respiratory tissue engineering2018

    • Author(s)
      土谷 智史
    • Organizer
      第54回日本肺サーファクタント・界面医学会学術研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] The transition of the extracellular matrix and alpha-gal antigens in the rat lung scaffold reseeded using human vascular and adipogenic stromal cells2018

    • Author(s)
      橋本泰匡
    • Organizer
      第20回異種移植研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 3次元構築を保った肺再生の取り組みとヒト肺創出への道2018

    • Author(s)
      土谷智史
    • Organizer
      第17回再生医療学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Potential of Adipose-Derived Stem/Stromal Cells for Lung Transplantation and Lung Tissue Engineering2018

    • Author(s)
      土谷智史
    • Organizer
      American Thoracic Society 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Remodeling the vasculature in the decellularized lung scaffold using adipogenic stromal cells2018

    • Author(s)
      土谷智史
    • Organizer
      第5回国際組織工学・再生医療学会 世界会議2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脱細胞化、再細胞化肺の免疫原性、細胞外マトリックスに対する効果2017

    • Author(s)
      橋本泰匡
    • Organizer
      第19回日本異種移植研究会
    • Place of Presentation
      メルパルク京都 (京都府京都市)
    • Year and Date
      2017-02-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラット肺における脱細胞化、再細胞化のα-gal抗原、細胞外マトリックスに対する影響2017

    • Author(s)
      橋本泰匡
    • Organizer
      第33回日本肺および心肺移植研究会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] 脱細胞組織骨格を利用した肺の再生法とその問題点2017

    • Author(s)
      土谷智史
    • Organizer
      第40回呼吸器内視鏡学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 臓器再生第一段階としての肺-脱細胞化組織骨格内の血管網の構築2016

    • Author(s)
      土谷智史
    • Organizer
      第43回日本臓器保存生物医学会学術集会
    • Place of Presentation
      東京薬科大学 (東京都八王子市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラット脱細胞化肺における細胞外マトリックス及び異種抗原の評価2016

    • Author(s)
      橋本泰匡
    • Organizer
      第4回MatriCellフォーラム
    • Place of Presentation
      東京理科大学 (東京都新宿区)
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] Adipose-derived stromal cells faciliate ex vivo angiogenesis and endothelial cell survival with pericyte differentiation in the decellularized rat lungs2016

    • Author(s)
      土肥良一郎
    • Organizer
      International Society for Stem Cell Research 2016
    • Place of Presentation
      サンフランシスコ (USA)
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] 再生医療の開発戦略と最新研究事例集2019

    • Author(s)
      高簿一弘
    • Total Pages
      419
    • Publisher
      技術情報協会
    • Related Report
      2018 Annual Research Report
  • [Remarks] 肺再生、気管再生

    • URL

      http://www.med.nagasaki-u.ac.jp/surgery1/introduction/research.html#saisei

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 脱細胞化用界面活性剤2018

    • Inventor(s)
      土谷智史ほか
    • Industrial Property Rights Holder
      土谷智史ほか
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 疾患モデル2018

    • Inventor(s)
      土谷智史ほか
    • Industrial Property Rights Holder
      土谷智史ほか
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2022-10-07  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi