| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
This study has focused on novel communication factor exosomes including microRNA (miRNA) in osteoarthritis (OA). We performed miRNA expression profiling in chondrocytes and exosomes using mesenchymal stem cells (MSC), normal- and OA-derived chondrocytes, and also performed glycan profiling using these samples. Several miRNAs were highly expressed in normal chondrocytes and chondrocytes-derived exosomes. These miRNAs were decreased in OA chondrocytes and OA chondrocytes-derived exosomes. Furthermore, MSC-derived exosome-formed miRNA reduced severity of OA in OA model mice. Thus, we newly generated these miRNAs knockout and cartilage specific transgenic mice. To examine the effects of chondrocytes-derived exosomes, pre-osteoclasts, pre-osteoblasts and pre-adipocytes were treated with chondrocytes-derived exosomes. Chondrocytes-derived exosomes inhibited osteoclastogenesis. These data suggest that exosomes including miRNA may play an important role in the pathogenesis and treatment of OA.
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