• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of novel communication factor exosomes for the treatment and diagnosis of osteoarthritis

Research Project

Project/Area Number 15H04959
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionHiroshima University

Principal Investigator

MIYAKI Shigeru  広島大学, 病院(医), 講師 (10392490)

Co-Investigator(Kenkyū-buntansha) 加藤 義雄  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (20415657)
石川 正和  広島大学, 医歯薬保健学研究科(医), 助教 (60372158)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords変形性関節症 / エクソソーム / マイクロRNA / 軟骨細胞 / 糖鎖 / マイクロENA
Outline of Final Research Achievements

This study has focused on novel communication factor exosomes including microRNA (miRNA) in osteoarthritis (OA). We performed miRNA expression profiling in chondrocytes and exosomes using mesenchymal stem cells (MSC), normal- and OA-derived chondrocytes, and also performed glycan profiling using these samples. Several miRNAs were highly expressed in normal chondrocytes and chondrocytes-derived exosomes. These miRNAs were decreased in OA chondrocytes and OA chondrocytes-derived exosomes. Furthermore, MSC-derived exosome-formed miRNA reduced severity of OA in OA model mice. Thus, we newly generated these miRNAs knockout and cartilage specific transgenic mice. To examine the effects of chondrocytes-derived exosomes, pre-osteoclasts, pre-osteoblasts and pre-adipocytes were treated with chondrocytes-derived exosomes. Chondrocytes-derived exosomes inhibited osteoclastogenesis. These data suggest that exosomes including miRNA may play an important role in the pathogenesis and treatment of OA.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (13 results)

All 2018 2016 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Int'l Joint Research] スクリプス研究所(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] スクリプス研究所(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Carnosic acid attenuates cartilage degeneration through induction of heme oxygenase-1 in human articular chondrocytes.2018

    • Author(s)
      Ishitobi H, Sanada Y, Kato Y, Ikuta Y, Shibata S, Yamasaki S, Lotz MK, Matsubara K, Miyaki S, Adachi N.
    • Journal Title

      Eur J Pharmacol.

      Volume: 830 Pages: 1-8

    • DOI

      10.1016/j.ejphar.2018.04.018

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Extracellular vesicles in cartilage homeostasis and osteoarthritis.2018

    • Author(s)
      Miyaki S, Lotz MK.
    • Journal Title

      Curr Opin Rheumatol.

      Volume: 1 Issue: 1 Pages: 129-135

    • DOI

      10.1097/bor.0000000000000454

    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Journal Article] <b>The role of tetraspanin CD9 in osteoarthritis using three different mouse </b><b>models </b>2016

    • Author(s)
      Sumiyoshi N, Ishitobi H, Miyaki S, Miyado K, Adachi N, Ochi M.
    • Journal Title

      Biomedical Research

      Volume: 37 Issue: 5 Pages: 283-291

    • DOI

      10.2220/biomedres.37.283

    • NAID

      130005432956

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mesenchymal Stem Cell-Derived Exosomes Promote Fracture Healing in a Mouse Model2016

    • Author(s)
      TAISUKE FURUTA, SHIGERU MIYAKI, HIROYUKI ISHITOBI, TOSHIHIKO OGURA, YOSHIO KATO, NAOSUKE KAMEI, KENJI MIYADO, YUKIHITO HIGASHI, MITSUO OCHI
    • Journal Title

      STEM CELLS TRANSLATIONALMEDICINE

      Volume: 5 Issue: 12 Pages: 1620-1630

    • DOI

      10.5966/sctm.2015-0285

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Articular chondrocytes-derived EVs regulate osteoclastogenesis, but not osteogenesis2018

    • Author(s)
      Sanada Y, Miyaki S, Adachi N.
    • Organizer
      International Society for Extracellular Vesicles
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Senescence accelerated mice as a new mouse model for spontaneous osteoarthritis2018

    • Author(s)
      Sanada Y, Miyaki S, Ikuta Y, Ishitobi H, Shinohara M, Nagira K, Ishikawa M, Nakasa T, Matsubara K, Lotz MK, Adachi N.
    • Organizer
      Osteoarthritis Research Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Histological scoring system for periarticular bone changes in mouse models of osteoarthritis2018

    • Author(s)
      Nagira K, Miyaki S, Ikuta Y, Lotz MK.
    • Organizer
      Osteoarthritis Research Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シンポジウム「OA治療標的分子としてのHO-1とmicroRNAの可能性」2016

    • Author(s)
      味八木 茂
    • Organizer
      第31回日本整形外科学会基礎学術集会
    • Place of Presentation
      福岡国際会議場(福岡市博多区)
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] OA治療標的分子としてのHO-1とmicroRNAの可能性2016

    • Author(s)
      味八木茂、安達伸生
    • Organizer
      第31回日本整形外科学会基礎学術集会
    • Place of Presentation
      福岡国際会議場(福岡市博多区)
    • Year and Date
      2016-10-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 間葉系幹細胞由来エクソソームは新たな組織再生因子である2016

    • Author(s)
      味八木茂、古田太輔、中邑祥博、石飛博之、越智光夫
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪国際会議場(大阪市北区)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 変形性関節症におけるmicroRNAを含むエクソソーム2016

    • Author(s)
      味八木 茂
    • Organizer
      第29回日本軟骨代謝学会
    • Place of Presentation
      広島大学広仁会館(広島市南区)
    • Year and Date
      2016-02-19
    • Related Report
      2015 Annual Research Report
    • Invited

URL: 

Published: 2015-04-16   Modified: 2022-10-07  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi