• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of an innovative imaging system for clarifying cell-cell interactions linking cancer bone metastasis and bone metabolism

Research Project

Project/Area Number 15H04962
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionEhime University

Principal Investigator

Imamura Takeshi  愛媛大学, 医学系研究科, 教授 (70264421)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsバイオテクノロジー / シグンル伝達 / 遺伝子 / タンパク質 / 細胞・組織
Outline of Final Research Achievements

In order to visualize and analyze the intercellular interaction of cancer cells and microenvironmental cells in breast cancer bone metastases in living mouse, we developed an innovative fluorescence imaging system of multicolor imaging with high spatial resolution or high temporal resolution within the bone marrow. Furthermore, we established bone metastasis models using breast cancer cell lines expressing various fluorescent proteins, and successfully imaged cancer cells and microenvironment including blood vessels, stromal cells, matrix collagen in the bone marrow. Finally, in bone marrow, we identified breast cancer cells that are involved in resistance to anticancer drugs that survive administration of 5 FU of anticancer drugs, and succeeded in imaging the interaction with various microenvironmental cells.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (9 results)

All 2018 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 6 results)

  • [Journal Article] Quantitative Morphometry for Osteochondral Tissues Using Second Harmonic Generation Microscopy and Image Texture Information.2018

    • Author(s)
      Saitou T, Kiyomatsu H, Imamura T.
    • Journal Title

      Sci Rep.

      Volume: 8(1) Issue: 1 Pages: 2826-2826

    • DOI

      10.1038/s41598-018-21005-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM).2016

    • Author(s)
      Yamamoto S, Oshima Y, Saitou T, Watanabe T, Miyake T, Yoshida O, Tokumoto Y, Abe M, Matsuura B, Hiasa Y, Imamura T
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 8 Pages: 277-283

    • DOI

      10.1016/j.bbrep.2016.09.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analyses of bone modeling and remodeling using in vitro reconstitution system with two-photon microscopy.2015

    • Author(s)
      Hikita A, Iimura T, Oshima Y, Saitou T, Yamamoto S, Imamura T
    • Journal Title

      Bone

      Volume: 76 Pages: 5-17

    • DOI

      10.1016/j.bone.2015.02.030

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 革新的蛍光イメージング技術が拓く次世代病理学2017

    • Author(s)
      今村健志
    • Organizer
      第106回日本病理学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 骨・軟骨イメージングのための顕微鏡開発2016

    • Author(s)
      今村健志
    • Organizer
      第31回日本整形外科学会基礎学術集会
    • Place of Presentation
      福岡市、福岡国際会議場
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] In vivo cancer imaging by advanced multi-photon laser excitation microscopy2016

    • Author(s)
      Takeshi Imamura
    • Organizer
      The 41st Naito Conference on Cancer Heterogeneity and Plasticity: Relevance to Therapeutic Resistance
    • Place of Presentation
      札幌市、シャトレーゼガトーキングダムサッポロ
    • Year and Date
      2016-07-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development and application of advanced intravital imaging technique in oncology and lymphology2016

    • Author(s)
      Takeshi Imamura
    • Organizer
      The 40th Annual meeting of the Japanese Society of Lymphology
    • Place of Presentation
      Ito International Research Center, The University of Tokyo
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 脳神経外科手術応用を目指した革新的バイオイメージング技術開発2016

    • Author(s)
      今村健志
    • Organizer
      第16回日本術中画像情報学会
    • Place of Presentation
      愛媛県松山市、松山全日空ホテル
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 先端的バイオイメージング技術の薬理学応用2016

    • Author(s)
      今村健志
    • Organizer
      第69回日本薬理学会西南部会
    • Place of Presentation
      愛媛県松山市、松山大学
    • Related Report
      2016 Annual Research Report
    • Invited

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi