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Inflammasome mediated neuroinflammation as a novel target of chronic pain

Research Project

Project/Area Number 15H04969
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Fumimasa Amaya  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60347466)

Co-Investigator(Kenkyū-buntansha) 岡林 志帆子  京都府立医科大学, 医学部附属病院, 研究員 (00744898)
佐和 貞治  京都府立医科大学, 医学(系)研究科(研究院), 教授 (10206013)
大橋 憲太郎  岐阜大学, 工学部, 准教授 (50332953)
澤田 麻衣子  京都府立医科大学, 医学(系)研究科(研究院), 助教 (90330860)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsインフラマソーム / 神経炎症 / 痛覚過敏 / 後根神経節
Outline of Final Research Achievements

The aim of this study was to investigate the hypothesis that activation of inflammasome in the DRG contributes to the development of chronic pain hypersensitivity.
NLRP2 expression and caspase1 activity was measured in DRG of chronic inflammation model mouse. Both NLRP2 expression and caspase1 activity was increased by chronic inflammation. Caspase1 inhibitor and NLRP2 siRNA reduced pain hypersensitivity during chronic inflammation.
These results demonstrated that NLRP2 inflammasome in the DRG contributes to the development of chronic inflammatory pain hypersensitivity.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (18 results)

All 2018 2017 2016 2015

All Journal Article (13 results) (of which Peer Reviewed: 13 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (5 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] Synergistic activation of ERK1/2 between A-fiber neurons and glial cells in the DRG contributes to pain hypersensitivity after tissue injury.2018

    • Author(s)
      Yamakita S, Horii Y, Takemura H, Matsuoka Y, Yamashita A, Yamaguchi Y, Matsuda M, Sawa T, Amaya F
    • Journal Title

      Mol Pain

      Volume: 14 Pages: 1-10

    • DOI

      10.1177/1744806918767508

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional validation of ATF4 and GADD34 in Neuro2a cells by CRISPR/Cas9-mediated genome editing.2018

    • Author(s)
      Oh-hashi K, Sugiura N, Amaya F, Isobe KI, Hirata Y.
    • Journal Title

      Mol Cell Biochem.

      Volume: 440 Issue: 1-2 Pages: 65-75

    • DOI

      10.1007/s11010-017-3156-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dexmedetomidine prolongs levobupivacaine analgesia via inhibition of inflammation and p38 MAPK phosphorylation in rat dorsal root ganglion2017

    • Author(s)
      Yamakita Shunsuke、Matsuda Megumi、Yamaguchi Yosuke、Sawa Teiji、Amaya Fumimasa
    • Journal Title

      Neuroscience

      Volume: 361 Pages: 58-68

    • DOI

      10.1016/j.neuroscience.2017.08.011

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acquired Exchange Protein Directly Activated by Cyclic Adenosine Monophosphate Activity Induced by p38 Mitogen-activated Protein Kinase in Primary Afferent Neurons Contributes to Sustaining Postincisional Nociception2017

    • Author(s)
      Matsuda Megumi、Oh-hashi Kentaro、Yokota Isao、Sawa Teiji、Amaya Fumimasa
    • Journal Title

      Anesthesiology

      Volume: 126 Issue: 1 Pages: 150-162

    • DOI

      10.1097/aln.0000000000001401

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Risk factors and prognosis of pain events during mechanical ventilation: a retrospective study2017

    • Author(s)
      Yamashita A, Yamasaki M, Matsuyama H, Amaya F
    • Journal Title

      J Intensive Care

      Volume: 5 Issue: 1 Pages: 17-17

    • DOI

      10.1186/s40560-017-0212-5

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Application of NanoLuc to monitor the intrinsic promoter activity of GRP78 using the CRISPR/Cas9 system2016

    • Author(s)
      Oh-Hashi K, Furuta E, Norisada J, Amaya F, Hirata Y, Kiuchi K.
    • Journal Title

      Genes Cells

      Volume: 10 Issue: 10 Pages: 1137-1143

    • DOI

      10.1111/gtc.12401

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative Effects of Periarticular Multimodal Drug Injection and Single-Shot Femoral Nerve Block on Pain Following Total Knee Arthroplasty and Factors Influencing Their Effectiveness2016

    • Author(s)
      Nakagawa S, Arai Y, Inoue H, Kan H, Hino M, Ichimaru S, Ikoma K, Fujiwara H, Amaya F, Sawa T, Kubo T
    • Journal Title

      Knee Surg Relat Res.

      Volume: 3 Issue: 3 Pages: 233-8

    • DOI

      10.5792/ksrr.2016.28.3.233

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effective evaluation of arterial pulse waveform analysis by two-dimensional stroke volume variation-stroke volume index plots2016

    • Author(s)
      Sawa T, Kinoshita M, Kainuma A, Akiyama K, Naito Y, Kato H, Amaya F, Shigemi K
    • Journal Title

      Journal of Clinical Monitoring and Computing

      Volume: 30 Issue: 5 Pages: 1-15

    • DOI

      10.1007/s10877-016-9916-7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cellular Stress Responses and Monitored Cellular Activities.2016

    • Author(s)
      Sawa T, Naito Y, Kato H, Amaya F.
    • Journal Title

      Shock

      Volume: 46 Issue: 2 Pages: 113-121

    • DOI

      10.1097/shk.0000000000000603

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Comparative Analysis of the Molecular Features of MANF and CDNF2016

    • Author(s)
      Norisada J, Hirata Y, Amaya F, Kiuchi K, Oh-Hashi K
    • Journal Title

      PLoS One

      Volume: 11 Issue: 1 Pages: e0146923-e0146923

    • DOI

      10.1371/journal.pone.0146923

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cellular Stress Responses and Monitored Cellular Activities.2016

    • Author(s)
      Sawa T, Naito Y, Katoh H, Amaya F.
    • Journal Title

      Shock

      Volume: -

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Can acute pain treatment reduce postsurgical comorbidity after breast cancer surgery? A literature review.2015

    • Author(s)
      Amaya F, Hosokawa T, Okamoto A, Matsuda M, Yamaguchi Y, Yamakita S, Taguchi T, Sawa T.
    • Journal Title

      Biomed Res Int.

      Volume: 2015 Pages: 641508-641508

    • DOI

      10.1155/2015/641508

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] mTOR signaling controls VGLUT2 expression to maintain pain hypersensitivity after tissue injury.2015

    • Author(s)
      Izumi Y, Sasaki M, Hashimoto S, Sawa T, Amaya F.
    • Journal Title

      Neuroscience.

      Volume: 308 Pages: 169-179

    • DOI

      10.1016/j.neuroscience.2015.09.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Endogenous cannabinoids contribute to the resolution of incisional pain2017

    • Author(s)
      H. TAKEMURA, M. MATSUDA, F. AMAYA
    • Organizer
      Society for Neuroscience annual meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression of NLRP2 in the DRG and its regulation during inflammatory hyperalgesia2017

    • Author(s)
      Y. MATSUOKA, F. AMAYA
    • Organizer
      Society for Neuroscience annual meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Increased caspase1 activity in the DRG during inflammatory hyperalgesia2016

    • Author(s)
      Y. MATSUOKA, K. OH-HASHI, F. AMAYA
    • Organizer
      Society for Neuroscience annual meeting 2016
    • Place of Presentation
      米国サンディエゴ
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EPAC is responsive for the exacerbation of the injury-induced pain hypersensitivity after the second surgery2016

    • Author(s)
      M. MATSUDA, F. AMAYA
    • Organizer
      Society for Neuroscience annual meeting 2016
    • Place of Presentation
      米国サンディエゴ
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bench to Bedsideレビュー:手術後に生じる知覚神経の機能変化2015

    • Author(s)
      天谷文昌
    • Organizer
      第2回日本区域麻酔学会学術集会
    • Place of Presentation
      高崎
    • Year and Date
      2015-04-25
    • Related Report
      2015 Annual Research Report
    • Invited

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Published: 2015-04-16   Modified: 2019-03-29  

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