| Project/Area Number |
15H04995
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
池田 康博 九州大学, 医学研究院, 准教授 (20380389)
星野 友 九州大学, 工学研究院, 准教授 (40554689)
吉田 茂生 九州大学, 大学病院, 助手 (50363370)
中尾 新太郎 九州大学, 大学病院, 講師 (50583027)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
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| Keywords | 糖尿病網膜症 / ROCK / ペリオスチン / 線維化 / 透過性 / 血管内皮細胞 / テネイシンC / 上皮間葉移行 / SARA / Smad2/3 / バリアー機能 / リパスジル / 網膜血管新生 / 血管透過性 / プラスチック抗体 |
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| Outline of Final Research Achievements |
We aimed to establish a novel molecular targeting therapy based on two important pathological conditions, retinal fibrovascular memmbrane formation and macular edema in diabetic retinopathy. We have clarified the involvement of tenascin-C whose expression is specifically enhanced in retinal fibrovascular proliferation. In addition, RNAi tarageting tenascin-C showed significant inhibitory effect on retinal fibrovascular proliferation. On the other hand, inflammatory cytokine stimulation including MCP-1, IL-6, TNF-a was performed after pre-administration of ROCK-inhibitor, Lipasdir, to the cultured vascular endothelial cells. The inhibiton of ROCK pathway resulted in a decrease in transendothelial electric resistance and claudin-5 expression. These results indicated that Lipasdir may inhibit disruption of barrier function by supressing inflammatory cytokines other than VEGF.
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