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Applied research for new preventive strategy for congenital retinal degenerative diseases using differentiation / regeneration technology

Research Project

Project/Area Number 15H04998
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionNational Rehabilitation Center for Persons with Disabilities

Principal Investigator

SEKO YUKO  国立障害者リハビリテーションセンター(研究所), 研究所 感覚機能系障害研究部, 研究部長 (60301157)

Co-Investigator(Kenkyū-buntansha) 東 範行  国立研究開発法人国立成育医療研究センター, 感覚器・形態外科部, 診療部長 (10159395)
梅澤 明弘  国立研究開発法人国立成育医療研究センター, 再生医療センター, 副所長/再生医療センター長 (70213486)
Project Period (FY) 2015-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords網膜視細胞 / ダイレクト・リプログラミング / ヒト体細胞 / 網膜変性疾患 / 網膜再生 / 網膜色素変性 / 眼発生 / 再生医療 / 細胞モデル / ゼブラフィッシュモデル / EYS / 再生医学 / 直接的分化誘導 / EYS遺伝子 / 網羅的遺伝子発現解析 / 変性視細胞モデル / ウィルスベクター / ゼブラフィッシュ / 眼発生・再生医学 / ダイレクト・リプログラミンング
Outline of Final Research Achievements

Direct reprogramming or redirect differentiation of somatic cells is a promising, simple, low-cost approach to generate target cells from somatic cells. We previously demonstrated that transduction of the combination, CRX, RAX, NeuroD and OTX2 genes, up-regulated expression of the photoreceptor-specific genes in human fibroblasts, where a photoresponse was detected. We here revealed that human peripheral blood mononuclear cells (PBMCs) can also be converted into photosensitive photoreceptor-like cells. We next produced photoreceptor-directed fibroblasts from retinitis pigmentosa patients as a replacement for the degenerative retinas using this differentiation technique. We analyzed defective transcripts of the causative gene in these cells. The results suggested that the redirect differentiation method could be a valuable tool for disease modeling despite some limitations. Major limitation of this method may be relatively higher heterogeneity in the induced photoreceptor-like cells.

Academic Significance and Societal Importance of the Research Achievements

人工多能性幹細胞(iPSc)作製(Takahashi and Yamanaka, 2006)と同様の方法で、転写因子遺伝子ミックス(CRX,NeuroD1,RAX)をレトロウィルスベクターによってヒト虹彩細胞に導入し光応答のある視細胞様細胞へ分化誘導することができた。OTX2の追加、あるいは細胞質型RNAウィルスベクターを用いることで、汎用性の高いヒト線維芽細胞やヒト末梢血単核細胞からも視細胞様細胞を作製した。また、網膜色素変性患者由来の皮膚線維芽細胞から変性視細胞モデルを作製し、病態解析の一助になること、臨床応用への可能性を示した。しかし、誘導細胞の細胞ごとの性質の違いは課題として残された。

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (15 results)

All 2019 2018 2016 2015

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Journal Article] X-linked Retinitis Pigmentosa in Japan: Clinical and Genetic Findings in Male Patients and Female Carriers2019

    • Author(s)
      Kurata Kentaro、Hosono Katsuhiro、Hayashi Takaaki、Mizobuchi Kei、Katagiri Satoshi、Miyamichi Daisuke、Nishina Sachiko、Sato Miho、Azuma Noriyuki、Nakano Tadashi、Hotta Yoshihiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 6 Pages: 1518-1518

    • DOI

      10.3390/ijms20061518

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis.2019

    • Author(s)
      Iwanami M, Oishi A, Ogino K, Seko Y, Nishida-Shimizu T, Yoshimura N, Kato S
    • Journal Title

      Mol Vis

      Volume: 25 Pages: 766-779

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intra- and interspecies comparison of EYS transcripts highlights its characteristics in the eye2019

    • Author(s)
      Shimpei Takita, Kiyoko Miyamoto-Matsui, Yuko Seko
    • Journal Title

      The FASEB Journal

      Volume: 印刷中 Issue: 8 Pages: 9422-9433

    • DOI

      10.1096/fj.201900056rr

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The manner of decay of genetically defective EYS gene transcripts in photoreceptor-directed fibroblasts derived from retinitis pigmentosa patients depends on the type of mutation.2018

    • Author(s)
      Seko Y, Iwanami M, Miyamoto-Matsui K, Takita S, Aoi N, Umezawa A, Kato S
    • Journal Title

      Stem Cell Res Ther

      Volume: 9 (1) : 279

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Variation in the phenotype of photosensitive cells produced from human fibroblast cell lines2018

    • Author(s)
      Ishii T, Yin C, Seko Y, Umezawa A, Kaneda M
    • Journal Title

      J Nippon Med Sch

      Volume: 85 (2) Pages: 110-116

    • NAID

      130006731160

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro transdifferentiation of human peripheral blood mononuclear cells to photoreceptor-like cells.2016

    • Author(s)
      Komuta Y, Ishii T, Kaneda M, Ueda Y, Miyamoto K, Toyoda M, Umezawa A, Seko Y,
    • Journal Title

      Biology Open

      Volume: 5(6) Pages: 709-719

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anteroposterior Patterning of Gene Expression in the Human Infant Sclera: Chondrogenic Potential and Wnt Signaling.2016

    • Author(s)
      Seko Y, Azuma N, Yokoi T, Kami D, Ishii R, Nishina S, Toyoda M, Shimokawa H, Umezawa A.
    • Journal Title

      Cur Eye Res

      Volume: 0000 Pages: 0000-0000

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro transdifferentiation of human peripheral blood mononuclear cells to photoreceptor-like cells.2016

    • Author(s)
      Komuta Y, Ishii T, Kaneda M, Ueda Y, Miyamoto K, Toyoda M, Umezawa A, Seko Y
    • Journal Title

      Biology Open

      Volume: May 11 Issue: 6 Pages: 709-719

    • DOI

      10.1242/bio.016477

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Remarkable decrease of rbp1 expression as hallmark of zebrafish eye with digenic eye+/-;;lrp5+/ -; retinitis pigmentosa ; candidate mutations2019

    • Author(s)
      Takita S and Seko Y
    • Organizer
      ARVO
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Global gene expression analysis of zebrafish eye with digenic eys+/-; lrp5+/- retinitis pigmentosa-candidate mutations2018

    • Author(s)
      Takita S and Seko Y
    • Organizer
      XXIII Biennial Meeting of the International Society for Eye Research – ISER
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Insights into EYS protein function(s) by deciphering the novel transcripts expressed in human dermal fibroblasts and zebrafish eye2018

    • Author(s)
      Takita S, Miyamoto-Matsui K and Seko Y
    • Organizer
      ARVO
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 網膜リプログラミング研究の進展2016

    • Author(s)
      世古裕子
    • Organizer
      第15回再生医療学会総会 シンポジウム10「幹細胞・リプログラミング研究の新展開」
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Generation and analysis of induced photoreceptor-like cells from fibroblasts of patients with retinitis pigmentosa2016

    • Author(s)
      Seko Y
    • Organizer
      Biennial Meeting of the International Society for Eye Research – ISER
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒト体細胞から網膜視細胞へのダイレクトリプログラミング2015

    • Author(s)
      世古裕子
    • Organizer
      感覚器セミナー
    • Place of Presentation
      東京
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 末梢血単核細胞から網膜視細胞様細胞への直接的分化誘導2015

    • Author(s)
      小牟田 縁、石井 俊行、金田 誠、上田 泰次、豊田 雅士、梅澤 明弘、世古 裕子
    • Organizer
      第38回 日本分子生物学会・第88回 日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2022-11-04  

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