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Stemness and tumorigenesis of salivary gland myoepithelial cells

Research Project

Project/Area Number 15H05013
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionShowa University

Principal Investigator

Mishima Kenji  昭和大学, 歯学部, 教授 (50275343)

Co-Investigator(Kenkyū-buntansha) 入江 太朗  岩手医科大学, 歯学部, 教授 (00317570)
安原 理佳  昭和大学, 歯学部, 講師 (20453649)
田中 準一  昭和大学, 歯学部, 助教 (40710166)
馬渕 洋  東京医科歯科大学, 大学院保健衛生学研究科, 助教 (50424172)
斎藤 一郎  鶴見大学, 歯学部, 教授 (60147634)
桐田 忠昭  奈良県立医科大学, 医学部, 教授 (70201465)
阪井 丘芳  大阪大学, 歯学研究科, 教授 (90379082)
福島 美和子  昭和大学, 歯学部, 助教 (90548273)
河野 葉子  昭和大学, 歯学部, 准教授 (40195681)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywords唾液腺 / 再生 / 筋上皮細胞 / 幹細胞 / 再生医療 / 多分化能 / 腫瘍原性
Outline of Final Research Achievements

We attempted to identify stem/progenitor cells in salivary gland. Localization of BrdU label-retaining cells were found specifically in myoepthelial cells located between luminal and stroma cells after 30 weeks, suggesting that myoepithelial cells were expected to be one of putative stem cells. The data from RNA-seq showed CD49fhigh subset contained positive cells for both of αSMA and Myh11, a marker of myoepithelial cells, demonstrating that CD49fhigh subset consisted of myoepithelial cell-enriched population. The selection of a CD49fhigh subset showed high ability of sphere formation and was differentiated into ductal lineage in vitro. These data suggest that the enriched myoepithelial cell compartment may contain stem/progenitor cells in salivary glands since the cells enriched in CD49fhigh subset showed multipotent properties by sphere formation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (9 results)

All 2018 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] The Role of the Slc39a Family of Zinc Transporters in Zinc Homeostasis in Skin2018

    • Author(s)
      Bin Bum-Ho、Hojyo Shintaro、Seo Juyeon、Hara Takafumi、Takagishi Teruhisa、Mishima Kenji、Fukada Toshiyuki
    • Journal Title

      Nutrients

      Volume: 10 Issue: 2 Pages: 219-219

    • DOI

      10.3390/nu10020219

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of mouse gingival junctional epithelial cell line using a bioengineered tooth2018

    • Author(s)
      Seki T, Aizawa R, Tanaka J, Yajima-Himuroa S, Kato M, Tanaka K, Mishima K, Yamamoto M.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 047 Issue: 1 Pages: 167-172

    • DOI

      10.1016/j.bbrc.2018.02.047

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Requirement of zinc transporter ZIP10 for epidermal development: Implication of the ZIP10-p63 axis in epithelial homeostasis.2017

    • Author(s)
      1.Bin BH , Bhin J , Takaishia M , Toyoshima K , Kawamata S , Ito K , Hara T , Watanabe T , Irie T , Takagishi T, Lee SH , Jung HS, Rho S , Seo J , Choi DH, Hwang D , Koseki H , Ohara O , Sano S , Tsuji T , Mishima K , Fukada T.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 114 Issue: 46 Pages: 12243-12246

    • DOI

      10.1073/pnas.1710726114

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 8-Nitro-cGMP promotes bone growth through expansion of growth plate cartilage2017

    • Author(s)
      Hoshino Marie、Kaneko Kotaro、Miyamoto Yoichi、Yoshimura Kentaro、Suzuki Dai、Akaike Takaaki、Sawa Tomohiro、Ida Tomoaki、Fujii Shigemoto、Ihara Hideshi、Tanaka Junichi、Tsukuura Risa、Chikazu Daichi、Mishima Kenji、Baba Kazuyoshi、Kamijo Ryutaro
    • Journal Title

      Free Radic Biol Med

      Volume: 110 Pages: 63-71

    • DOI

      10.1016/j.freeradbiomed.2017.05.022

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Resveratrol improves salivary dysfunction in a non-obese diabetic (NOD) mouse model of Sjögren’s syndrome2016

    • Author(s)
      Inoue H, Kishimoto A, Nakayama RU, Hasaka A, Takahashi A, Ryo A, Muramatsu T, Ide F, Mishima K, Saito I
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 59 Issue: 2 Pages: 107-112

    • DOI

      10.3164/jcbn.16-31

    • NAID

      130005262395

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] MOUSE SALIVARY GLAND-DERIVED MYOEPITHELIAL CELLS SHOWS STEM/PROGENITOR CELL PROPERTIES IN VITRO2017

    • Author(s)
      Rika Yasuhara, Junichi Tanaka, Miwako Fukushima, Tarou Irie, Kenji Mishima
    • Organizer
      国際幹細胞学会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 唾液腺由来筋上皮細胞の単離と性質解析2016

    • Author(s)
      安原理佳、田中準一、福島美和子、入江太朗、河野葉子、美島健二
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 唾液腺由来筋上皮細胞の単離と性質解析2016

    • Author(s)
      Yasuhara R, Tanaka J, Fukushima M, IrieT , Kohno Y, Mishima K
    • Organizer
      105回日本病理学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 唾液腺由来筋上皮細胞の性質解析2015

    • Author(s)
      安原 理佳, 田中 準一, 福島 美和子, 入江 太朗, 河野 葉子, 美島 健二
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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