| Project/Area Number |
15H05027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Hiroshima University |
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Principal Investigator |
Hiroki NIKAWA 広島大学, 医歯薬保健学研究科(歯), 教授 (10228140)
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| Co-Investigator(Kenkyū-buntansha) |
田地 豪 広島大学, 医歯薬保健学研究科(歯), 准教授 (80284214)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | 口腔インプラント / DLC / 骨芽細胞 / 破骨細胞 / オッセオインテグレーション / Diamond-Like Carbon / インプラント |
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| Outline of Final Research Achievements |
To examine the effects of diamond like carbon coating containing different level of hydrogen (ta-C(H) DLC), on the differentiation of osteoblastic cells and osteoclastic cells in vitro. In addition, antimicrobial activity of ta-C(H) DLC was also examined.
Real-time quantitative reverse transcriptional polymerase chain reaction analysis revealed that ta-C(H) DLC promoted the expression of Runx 2, Osterix and ALP of MC3T3-E1 cells, as compared with those of untreated titanium. On the other hand, ta-C(H) DLC suppressed the expression of TRAP and cathepsin K mRNA in RAW264.7 cells, as compared with those of untreated titanium. Further, ta-C(H) DLC exhibited the inhibitory effect against P. gigivalis biofilm formation, as compared with that on untreated titanium. These results, taken together, suggested that ta-C(H) DLC is one of the most preferable avenue to obtain the acquisition of osseo-integration.
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