Analysis of molecular mechanisms of sensitization phase to develop effective treatments against metal allergy

• Project/Area Number: 15H05028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: The University of Tokushima
• Principal Investigator: ICHIKAWA Tetsuo 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (90193432)
• Co-Investigator(Kenkyū-buntansha): 渡邉 恵 徳島大学, 病院, 講師 (40380050) ; 石丸 直澄 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (60314879) ; 南 憲一 徳島大学, 病院, 医員 (60732914) ; 水頭 英樹 徳島大学, 病院, 助教 (70732915)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 歯科用金属アレルギー / 樹状細胞 / 上皮角化細胞 / 金属アレルギー / ケラチノサイト / 金属溶出

Outline of Final Research Achievements

The aim of this study is to clarify the mechanisms of sensitization phase of metal allergy development by analyzing the effect of dental metals upon immune cells. We showed dendritic cells (DC) can uptake nickel directly by using fluorescent particles that recognized nickel in vitro and same phenomenon was observed in skin. Expressions of CD100, MAPkinase, and RhoA in DC stimulated with nickel were increased subsequently. This suggested that these cascades might regulate DC’s migration after the uptake of Ni. In addition, expression of plexin B1 and plexin B2 were increased in keratinocytes stimulated with nickel. Therefore, interactions between plexin Bs and CD100 may play an important role in development of sensitization and these interactions have a possibility of using for diagnosis or treatment for metal allergy.

Research Products

• [Presentation] 義歯材料とアレルギー (2018)
  • Author(s): 渡邉 恵
  • Organizer: 日本義歯ケア学会 第10回学術大会
  • Invited
• [Presentation] ニッケルで刺激したケラチノサイトと樹状細胞におけるPlexin-Semaphorinの動態解析 (2017)
  • Author(s): 南憲一，渡邉 恵，市川哲雄
  • Organizer: （公社）日本補綴歯科学会 第126回学術大会
• [Presentation] 基礎研究からみた金属アレルギー (2016)
  • Author(s): 渡邉 恵
  • Organizer: 第8回 一般社団法人 日本メタルフリー歯科学会学術大会
  • Place of Presentation: 愛知学院大学（愛知県名古屋市）
  • Invited
• [Presentation] 金属アレルギーの発症と免疫 (2015)
  • Author(s): 渡邉 恵
  • Organizer: （公社）日本補綴歯科学会 関西支部総会・学術大会
  • Place of Presentation: 兵庫医療大学（兵庫県神戸市）
  • Year and Date: 2015-11-08
  • Invited
• [Presentation] 金属アレルギーの病態 (2015)
  • Author(s): 渡邉 恵
  • Organizer: （公社）日本補綴歯科学会第124回学術大会
  • Place of Presentation: 大宮ソニックシティ（埼玉県さいたま市）
  • Year and Date: 2015-05-30