A basic study for the development of stem cell-cytokine therapy aimed at the enhancing periodontal tissue regeneration effect.
| Project/Area Number |
15H05034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
村上 伸也 大阪大学, 歯学研究科, 教授 (70239490)
柳田 学 大阪大学, 歯学研究科, 招へい教員 (80379081)
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| Research Collaborator |
YAMADA Satoru
TAKEDACHI Masahide
SAWADA Keigo
ANZAI Jun
NAGAYASU Toshie
SAKASHITA Hiromi
YAMAMOTO Tomomi
TSUSHIMA Kenichiro
MORIMOTO Chiaki
HASEGAWA Shiori
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | 歯周病 / 再生 / サイトカイン / 幹細胞 |
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| Outline of Final Research Achievements |
In this study, we investigated the possibility of enhancing periodontal regeneration effect in the cell therapy using mesenchymal stem cells and the cytokine therapy. As a result, it was revealed that fibrin gel and β-3 calcium phosphate (β-TCP) whose granule size was 500-1000 mm were useful as a scaffold for adipose tissue-derived stem cells (ADSC). The proteomic analysis of culture supernatants of ADSC and bone marrow- derived mesenchymal stem cells suggested that Periostin might be involved in periodontal tissue regeneration. In addition, the bone regeneration was observed by application of FGF-2 in the fenestration model of beagle dogs. These results suggested that the combination of cell therapy using stem cells and cytokine therapy might enhance the periodontal tissue regeneration effect.
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Report
(4 results)
Research Products
(24 results)
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[Presentation] The mechanism of periodontal tissue regeneration by transplantation of adipose tissue-derived multi-lineage progenitor cells.2016
Author(s)
Keigo Sawada, Masahide Takedachi, Tomoaki Iwayama, Satomi Yamamoto, Chiaki Morimoto, Asae Hirai, Chun Man Lee, Hanayuki Okura, Akifumi Matsuyama, Yuko Sano, Masahiro Kitamura, Shinya Murakami
Organizer
International Society for Stem Cell Research 2016
Place of Presentation
San Francisco, USA
Year and Date
2016-06-22
Related Report
Int'l Joint Research
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[Presentation] Periodontal tissue regeneration by transplantation of adipose tissue-derived multi-lineage progenitor cells.2015
Author(s)
Keigo SAWADA, Masahide TAKEDACHI, Satomi YAMAMOTO, Chiaki MORIMOTO, Asae HIRAI, Masao OZASA, Tomoaki IWAYAMA, Yuko SANO, Masahiro KITAMURA, Shinya MURAKAMI
Organizer
nternational Symposium 2015, Oral and Craniofacial Development and Diseases
Place of Presentation
弓倉記念ホール（大阪府吹田市）
Year and Date
2015-12-10
Related Report
Int'l Joint Research
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[Presentation] Periodontal tissue regeneration by transplantation of ADMPC2015
Author(s)
Masahide TAKEDACHI, Keigo SAWADA, Satomi YAMAMOTO, Chiaki MORIMOTO, Asae HIRAI, Masao OZASA, Tomoaki IWAYAMA, Yuko SANO, Chun Man LEE, Akihumi MATSUYAMA, Hanayuki OKURA, Masahiro KiITAMURA, Shinya MURAKAMI
Organizer
第63回国際歯科研究学会学術大会
Place of Presentation
福岡国際会議場（福岡）
Year and Date
2015-10-30
Related Report
Int'l Joint Research
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