Establishment of tooth regeneration strategy by neural crest cells induced from human iPS cells
| Project/Area Number |
15H05037
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
Honda Masaki 愛知学院大学, 歯学部, 教授 (70361623)
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| Co-Investigator(Kenkyū-buntansha) |
鳥海 拓 愛知学院大学, 歯学部, 講師 (40610308)
磯川 桂太郎 日本大学, 歯学部, 教授 (50168283)
湯口 眞紀 日本大学, 歯学部, 助手 (00256885)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | tooth regeneration / neural crest cells / iPS cells / tissue engineering / dentin / cementum / 歯の再生 / iPS細胞 / エナメル質 / 象牙質 / セメント質 / 神経堤細胞 / 再生 / 歯 / 上皮幹細胞 / 歯髄幹細胞 / シュワン細胞 / 骨芽細胞 / 軟骨細胞 / エナメル芽細胞 / 再生医学 / 細胞，組織学 / 歯学 / 発生・分化 / 解剖学 / ヒトiPS細胞 / ヒト歯髄細胞 / 象牙芽細胞 / セメント芽細胞 / 移植 / ブタ歯胚 |
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| Outline of Final Research Achievements |
Tooth development is started from the interaction between epithelial and neural crest derived mesenchymal cells. We try to induce human iPS cells into neural crest cells to develop dental tissues. First, we are successfully induced the human iPS cells into neural crest cells. Then, the dentin and cementum formation are successfully generated from transplanted the combination both neural crest cells and dental epithelial cells in tissue engineering methods. These results indicated that the object in this study are almost achieved during three years.
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Report
(4 results)
Research Products
(59 results)
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[Journal Article] Effect of collagenase concentration on the isolation of small adipocytes from human buccal fat pad2018
Author(s)
Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T Kazama T, Oki Y, Saito-Tamura Y, Tonogi M, Shimizu N, Honda M
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Journal Title
Journal of Oral Science
Volume: 60
Issue: 1
Pages: 14-23
DOI
NAID
ISSN
1343-4934, 1880-4926
Related Report
Peer Reviewed / Open Access
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[Journal Article] Transplantation of mature adipocyte-derived dedifferentiated fat cells into three-wall defects in the rat periodontium induces tissue regeneration2017
Author(s)
Suzuki D, Akita D, Tsurumachi N, Kano K, Yamanaka K, Kaneko T, Kawano E, Iguchi S, Toriumi T, Arai Y, Matsumoto T, Sato S, Honda M
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Journal Title
Journal of Oral Science
Volume: 59
Issue: 4
Pages: 611-620
DOI
NAID
ISSN
1343-4934, 1880-4926
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effect of local bone marrow stromal cell administration on ligature-induced periodontitis in mice2017
Author(s)
Iguchi S, Suzuki D, Kawano E, Mashimo T, Kajiya M, Toriumi T, Kawai T, Kurihara H, Isokawa K, Sato S, Honda M
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Journal Title
Journal of Oral Science
Volume: 59
Issue: 4
Pages: 629-637
DOI
NAID
ISSN
1343-4934, 1880-4926
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effect of local bone marrow stromal cell administration on ligature-induced periodontitis in mice2017
Author(s)
Shinya Iguchi, Daigo Suzuki, Eisuke Kawano, Takayuki Mashimo, Mikihito Kajiya, Taku Toriumi, Toshihisa Kawai, Hidemi Kurihara, Keitaro Isokawa, Shuichi Sato, Masaki Honda
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Journal Title
Journal of Oral Sciences
Volume: in press
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Use of rat mature adipocyte-derived dedifferentiated fat cells as a cell source for periodontal tissue regeneration.2016
Author(s)
Akita D, Kano K, Saito-Tamura Y, Mashimo T, Sato-Shionome M, Tsurumachi N, Yamanaka K, Kaneko T, Toriumi T, Arai Y, Tsukimura N, Matsumoto T, Ishigami T, Isokawa K, Honda M
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Journal Title
Frontier Physiology
Volume: 7
Pages: 50-50
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Small buccal fat pad cells have high osteogenic differentiateon potential.2016
Author(s)
Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama T, Oki Y, Tamura Y,Tonogi M, Isokawa K, Shimizu N, Honda M
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Journal Title
Tissue Engineering Part C: Methods
Volume: 22
Issue: 3
Pages: 250-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Use of Rat Mature Adipocyte-Derived Deffirentiated Fat Cells as a Cell Source for Periodontal Tissue Regeneration.2016
Author(s)
Akita D, Kano K, Saito-Tamura Y, Mashimo T, Sato-Shionome M, Tsurumachi N, Yamanaka K, Kaneko T Toriumi T, Arai Y, Tsukimura N, Matsumoto T, Ishigami T, Iisokawa K, Honda M.
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Journal Title
Front Physiol
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Small Buccal Fat Pad Cells Have High Osteogenic Differentiation potential.2016
Author(s)
Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama T, oki Y, Tamura Y, Tonoji M, Iisokawa K, Shimizu N, Honda M.
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Journal Title
Tissue Eng Part C Methods
Volume: 22(3)
Pages: 250-9
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Characterization of mesenchymal progenitor cells in the crown and root pulp of primary teeth2015
Author(s)
Toriumi T, Takayama N, Murakami M, Sato M, Yuguchi M, Yamazaki Y, Eto K, Otsu M, Nakauchi H, Shirakawa T, Isokawa K, Honda MJ.
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Journal Title
Biomed Res
Volume: 36(1)
Pages: 31-45
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Use of BMSCs prevents alveolar bone resorption on experimental periodontitis2016
Author(s)
Iguchi S, Kawano E, Suzuki D, Tsurumachi N, Kajiya M, Toriumi T, Isokawa K, Arai Y, Fukuda T, Fujisaki Y, Sugano N, Sato S, Honda M
Organizer
The 102nd Annual Meeting of the American Academy of Periodontology in collaboration with the Japanese Society of Periodontology and Japanese academy of Clinical Periodontology
Place of Presentation
San Diego Convention Center (San Diego, CA, USA)
Year and Date
2016-09-10
Related Report
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[Presentation] Dedifferentiated fat cells for tissue engineering in rat periodontal defects2016
Author(s)
Suzuki D, Iguchi S, Kawano E, Tsurumachi N, Toriumi T, Isokawa K, Arai Y, Sakai A, Ooshio K, Sugano N, Sato S, Honda M
Organizer
The 102nd Annual Meeting of the American Academy of Periodontology in collaboration with the Japanese Society of Periodontology and Japanese academy of Clinical Periodontology
Place of Presentation
San Diego Convention Center (San Diego, CA, USA)
Year and Date
2016-09-10
Related Report
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