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Devlopment of novel oral cancer treatment with non-coding RNA by targetting metastatic organ and cancer stem cells.

Research Project

Project/Area Number 15H05045
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKagoshima University

Principal Investigator

Sugiura Tsuyoshi  鹿児島大学, 医歯学域歯学系, 教授 (40322292)

Co-Investigator(Kenkyū-buntansha) 浜田 倫史  鹿児島大学, 医歯学域附属病院, 講師 (00444894)
熊丸 渉  九州大学, 大学病院, 講師 (90432947)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Keywords口腔癌 / microRNA / 血清マーカー / 癌幹細胞 / 上皮間葉移行 / BRACHYURY / SOX2 / 癌 / マイクロRNA / Brachyury / 機能性RNA / 腫瘍マーカー / 口腔癌幹細胞
Outline of Final Research Achievements

Cancer cells produce short non-coding RNA and release to the body fluid. We discovered oral cancer specific microRNAs by comparing serum from normal and oral cancer indibiduals. These oral cancer specific microRNAs are the candidates for oral cancer biomarkers and we next analyzed quantitatively these microRNAs for each 40 normal and oral cancer serum samples. The oral cancer specific microRNA could diagnose oral cancer (sensitivity 67.5%) and normal individual (specificity 87.5%). This fact suggests the microRNA could be the serum marker for oral cancer.

Academic Significance and Societal Importance of the Research Achievements

本研究結果より血清microRNAにより口腔癌が診断できることが明らかとなった。口腔癌には腫瘍マーカーがなく、がん検診のような多数の住民を対象とした場合の第一段検査が無かったが、血液検査陽性個人にのみ第2段の精密検査を行うことを可能とする技術となる。さらに、口腔癌を治療した後の再発や転移についても血液検査により検出が可能である。口腔癌特異的microRNAは遺伝子発現を阻害する機能を持つため、将来的に治療に応用することも可能と考えられる。

Report

(4 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (28 results)

All 2018 2017 2016 2015

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 8 results,  Acknowledgement Compliant: 4 results) Presentation (17 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Journal Article] Transfection of T-Box Transcription Factor BRACHYURY and SOX2 Synergistically Promote Self-Renewal and Invasive Phenotype in Oral Cancer Cells2018

    • Author(s)
      Akimoto Naonari、Nakamura Kodai、Hijioka Hiroshi、Kume Kenichi、Matsumura Yoshiaki、Sugiura Tsuyoshi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 19 Issue: 11 Pages: 3620-3620

    • DOI

      10.3390/ijms19113620

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Deregulation of Nicotinamide N-Methyltransferase and Gap Junction Protein Alpha-1 Causes Metastasis in Adenoid Cystic Carcinoma2018

    • Author(s)
      Ishibashi, K., Ishii, K., Sugiura, T., Mori, Y.
    • Journal Title

      Anticancer Research

      Volume: 38 Issue: 1 Pages: 187-197

    • DOI

      10.21873/anticanres.12207

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland.2016

    • Author(s)
      Hayashi K, Ikari T, Sugiyama G, Sugiura T, Ohyama Y,Kumamaru W, Shirasuna K,Mori Y.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 477 Issue: 4 Pages: 814-819

    • DOI

      10.1016/j.bbrc.2016.06.140

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient regulation of branching morphogenesis via fibroblast growth factor receptor 2c in early-stage embryonic mouse salivary glands.2016

    • Author(s)
      Shibuya M., Ikari T., Sugiyama G., Ohyama Y., Kumamaru W., Nagano K., Sugiura T., Shirasuna K. and Mori Y.
    • Journal Title

      Differentiation

      Volume: 92(4) Issue: 4 Pages: 216-224

    • DOI

      10.1016/j.diff.2016.05.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expression levels of SOX2, KLF4 and brachyury transcription factors are associated with metastasis and poor prognosis in oral squamous cell carcinoma2016

    • Author(s)
      Yoshihama R, Yamaguchi K, Imajyo I, Mine M, Hiyake N, Akimoto N, Kobayashi Y, Chigita S, Kumamaru W, Kiyoshima T, Mori Y, Sugiura T
    • Journal Title

      Oncology letters

      Volume: 11 Issue: 2 Pages: 1435-1446

    • DOI

      10.3892/ol.2015.4047

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aberrant methylation of MUC1 and MUC4 promoters are potential prognostic biomarkers for pancreatic ductal adenocarcinomas2016

    • Author(s)
      Yokoyama, S. Higashi, M. Kitamoto, S. Oeldorf, M. Knippschild, U. Kornmann, M. Maemura, K. Kurahara, H. Wiest, E. Hamada, T. Kitazono, I. Goto, Y. Tasaki, T. Hiraki, T. Hatanaka, K. Mataki, Y. Taguchi, H. Hashimoto, S. Batra, S. K. Tanimoto, A. Yonezawa, S. Hollingsworth, M. A.
    • Journal Title

      Oncotarget

      Volume: - Issue: 27 Pages: 42553-42565

    • DOI

      10.18632/oncotarget.9924

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A Novel Function of CD82/KAI1 in Sialyl Lewis Antigen-Mediated Adhesion of Cancer Cells: Evidence for an Anti-Metastasis Effect by Down-Regulation of Sialyl Lewis Antigens.2015

    • Author(s)
      Yoshihama N, Yamaguchi K, Chigita S, Mine M, Abe M, Ishii K, Kobayashi Y, Akimoto N, Mori Y, Sugiura T
    • Journal Title

      PLoS One

      Volume: 10 Issue: 4 Pages: e0124743-e0124743

    • DOI

      10.1371/journal.pone.0124743

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] miR-203 Inhibits Frizzled-2 Expression via CD82/KAI1 Expression in Human Lung Carcinoma Cells.2015

    • Author(s)
      Mine M, Yamaguchi K, Sugiura T, Chigita S, Yoshihama N, Yoshihama R, Hiyake N, Kobayashi Y, Mori Y:
    • Journal Title

      PLoS One

      Volume: 10 Issue: 7 Pages: e0131350-e0131350

    • DOI

      10.1371/journal.pone.0131350

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Detecting oral squamous cell carcinoma by novel serum microRNA panel2018

    • Author(s)
      中村康大、杉浦剛
    • Organizer
      第77 回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規血清microRNA による口腔扁平上皮癌の検出2018

    • Author(s)
      中村康大、杉浦剛
    • Organizer
      第63 回公益社団法人口腔外科学会総会・学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] DNA methylation of tumor relatedgenes in gargled fluid: A noninvasive method for detecting oral precancerous lesion2018

    • Author(s)
      浜田倫史、杉浦剛
    • Organizer
      第77 回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 非侵襲的口腔検体検査のススメ 口腔がんから全身疾患まで、その有用性と展望2017

    • Author(s)
      杉浦 剛
    • Organizer
      第28 回近畿 中国 四国 口腔衛生学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Novel serum microRNAs specific for oral squamous cell carcinoma: its usefulness for early detection2017

    • Author(s)
      中村康大、杉浦剛
    • Organizer
      第76回 日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 口腔扁平上皮癌における血清microRNAの検出とその診断的意義2016

    • Author(s)
      中村康大、日焼菜央美、浜田倫史、杉浦剛
    • Organizer
      第34回日本口腔腫瘍学会総会・学術大会
    • Place of Presentation
      横浜開港記念会館(神奈川県横浜市)
    • Year and Date
      2016-01-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 口腔扁平上皮癌におけるMUC1/MUC4 ムチン共発現の予後規定因子としての有用性の検討2016

    • Author(s)
      浜田倫史, 上村祐二, 中村康大, 上川善昭, 杉浦剛
    • Organizer
      第70回NPO法人日本口腔科学会学術集会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 口腔癌早期発見に向けた当科の取り組み2016

    • Author(s)
      浜田倫史、中村康大、大河原綾子、比地岡浩志、石田喬之、久米健一、中村典史、杉浦剛
    • Organizer
      九州地区口腔癌研究会 第20回学術講演会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 口腔扁平上皮癌における血清microRNAの検出とその診断的意義2016

    • Author(s)
      中村康大、浜田倫史、杉浦剛
    • Organizer
      第34回日本口腔腫瘍学会総会・学術大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 口腔扁平上皮癌診断マーカーとしての血清microRNAの探索2016

    • Author(s)
      中村康大、浜田倫史、杉浦剛
    • Organizer
      第70回NPO法人日本口腔科学会学術集会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Combination of serum microRNAs for biomarker in oral squamous cell carcinoma2016

    • Author(s)
      Koudai Nakamura , Naomi Hiyake , Tomofumi Hamada , Tsuyoshi Sugiura
    • Organizer
      第75回日本癌学会学術総会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 口腔扁平上皮癌の検出に有用な血清microRNAの検討2016

    • Author(s)
      中村康大、日焼菜央美、松村吉晃、後藤雄一、浜田倫史、杉浦剛
    • Organizer
      第61回日本口腔外科学会総会・学術大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification of serum microRNAs as biomarker for oral squamous cell carcinoma2015

    • Author(s)
      日焼菜央美、杉浦剛、吉濱直哉、秋本直柔、峯真理子、吉濱るみ、森悦秀
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Downregulation of delta-Np63 induces a mesenchymal cell phenotype, possibly involoving invasion and metastasis of OSCC2015

    • Author(s)
      後藤雄一、河野新太郎、松原良太、橋口有真、坂元泰基、金子直樹、丸瀬靖之、神野哲平、中村康大、浜田倫史、大部一成、杉浦剛、中村誠司
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] MUC$ expression is correlated to cisplatin chemoresistance in oral squamouse cell carcinoma2015

    • Author(s)
      浜田倫史、中村康大、後藤雄一、杉浦剛
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 口腔扁平上皮癌患者における血清腫瘍マーカーmicroRNAの探索2015

    • Author(s)
      日焼菜央美、杉浦剛、峯真理子、吉濱るみ、秋本直柔、吉濱直哉、千北さとみ、小林洋輔、熊丸渉、森悦秀
    • Organizer
      第69回日本口腔科学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2015-05-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 含嗽液を用いた癌抑制遺伝子DNAメチル化異常の検出2015

    • Author(s)
      浜田倫史、熊谷直之、後藤雄一、上川善昭、杉浦剛
    • Organizer
      第69回日本口腔科学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2015-05-13
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 口腔癌判定装置、口腔癌判定方法、プログラム及び口腔癌判定キット2018

    • Inventor(s)
      中村康大、杉浦剛、浜田倫史
    • Industrial Property Rights Holder
      中村康大、杉浦剛、浜田倫史
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] METHOD FOR DETECTING PRECANCEROUS ORAL LESION2017

    • Inventor(s)
      HAMADA Tomofumi
    • Industrial Property Rights Holder
      HAMADA Tomofumi
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 口腔前癌病変の検出方法2017

    • Inventor(s)
      浜田倫史
    • Industrial Property Rights Holder
      浜田倫史
    • Industrial Property Rights Type
      特許
    • Patent Publication Number
      2017-046628
    • Filing Date
      2017
    • Acquisition Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2020-03-30  

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