Specification of route of Helicobacter pylori infection and construction of preventive approaches for gastric diseases in pediatric dentistry field
| Project/Area Number |
15H05049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
仲 周平 岡山大学, 大学病院, 講師 (10589774)
大川 玲奈 大阪大学, 歯学部附属病院, 講師 (80437384)
野村 良太 大阪大学, 歯学研究科, 准教授 (90437385)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Yoshiyuki 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00191809)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | ヘリコバクター・ピロリ菌 / ゲノム / PCR 法 / nested PCR 法 / ureA / 歯髄サンプル / 唾液サンプル / 歯髄線維芽細胞 / Nested PCR法 / 検出プライマー / 口腔サンプル / 感染歯髄 / デンタルプラーク / 除菌療法 / 十二指腸潰瘍 / ピロリ菌 / 感染経路 / 胃疾患 / 口腔 / 小児 / 乳歯 / 感染根管 / 分子生物学的手法 / 歯髄組織 / 定着 / 胃炎 / 胃潰瘍 / 感染 / 伝播 / う蝕 / 歯髄 / 成人 |
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| Outline of Final Research Achievements |
Helicobacter pylori, a Gram-negative microaerophilic bacteria, is known to be a major pathogen related to gastric diseases. The oral cavity is considered to be the source of H. pylori infection, which is mainly acquired in early childhood. In the present study, a novel PCR system for H. pylori detection was constructed using primer sets designed from the complete genome information of 48 H. pylori strains. The bacteria were identified in 38.9% of 131 inflamed pulp specimens obtained from children using the PCR system. In addition, H. pylori strains showed adhesion to human dental fibroblast cells.
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Report
(4 results)
Research Products
(10 results)
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[Presentation] Comparison of the detection of Helicobacter pylori in dental plaque from Japanese children and adults.2016
Author(s)
Ogaya Y, Nomura R, Matsui D, Watanabe I, Miyatani F, Iwai K, Ozaki E, Kuriyama N, Watanabe Y, Nakano K.
Organizer
94th General Session & Exhibition of the International Association for Dental Research
Place of Presentation
Seoul, Korea
Year and Date
2016-06-22
Related Report
Int'l Joint Research
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[Presentation] Distribution of Helicobacter pylori in dental plaque from Japanese children and adults.2016
Author(s)
Ogaya Y, Nomura R, Matsui D, Watanabe I, Koyama T, Miyatani F, Iwai K, Ozaki E, Kuriyama N, Watanabe Y, Nakano K.
Organizer
The 93rd General Session of the International Association for Dental Research
Place of Presentation
Seoul, Korea
Year and Date
2016-06-22
Related Report
Int'l Joint Research
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