| Project/Area Number |
15H05049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
仲 周平 岡山大学, 大学病院, 講師 (10589774)
大川 玲奈 大阪大学, 歯学部附属病院, 講師 (80437384)
野村 良太 大阪大学, 歯学研究科, 准教授 (90437385)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Yoshiyuki 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00191809)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | ヘリコバクター・ピロリ菌 / ゲノム / PCR 法 / nested PCR 法 / ureA / 歯髄サンプル / 唾液サンプル / 歯髄線維芽細胞 / Nested PCR法 / 検出プライマー / 口腔サンプル / 感染歯髄 / デンタルプラーク / 除菌療法 / 十二指腸潰瘍 / ピロリ菌 / 感染経路 / 胃疾患 / 口腔 / 小児 / 乳歯 / 感染根管 / 分子生物学的手法 / 歯髄組織 / 定着 / 胃炎 / 胃潰瘍 / 感染 / 伝播 / う蝕 / 歯髄 / 成人 |
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| Outline of Final Research Achievements |
Helicobacter pylori, a Gram-negative microaerophilic bacteria, is known to be a major pathogen related to gastric diseases. The oral cavity is considered to be the source of H. pylori infection, which is mainly acquired in early childhood. In the present study, a novel PCR system for H. pylori detection was constructed using primer sets designed from the complete genome information of 48 H. pylori strains. The bacteria were identified in 38.9% of 131 inflamed pulp specimens obtained from children using the PCR system. In addition, H. pylori strains showed adhesion to human dental fibroblast cells.
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