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Development of comprehensive evaluation of local and systemic condition by skin blotting

Research Project

Project/Area Number 15H05066
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fundamental nursing
Research InstitutionThe University of Tokyo

Principal Investigator

Minematsu Takeo  東京大学, 大学院医学系研究科(医学部), 特任准教授 (00398752)

Co-Investigator(Kenkyū-buntansha) 禰屋 光男  びわこ成蹊スポーツ大学, スポーツ学部, 准教授 (30359640)
山田 実  筑波大学, 人間系, 准教授 (30525572)
吉田 美香子  東京大学, 大学院医学系研究科(医学部), 特任講師 (40382957)
真田 弘美  東京大学, 大学院医学系研究科(医学部), 教授 (50143920)
野口 博史  東京大学, 大学院医学系研究科(医学部), 特任講師 (50431797)
仲上 豪二朗  東京大学, 大学院医学系研究科(医学部), 准教授 (70547827)
玉井 奈緒  東京大学, 大学院医学系研究科(医学部), 特任講師 (80636788)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywordsスキンアセスメント / スキンケア / スキンブロッティング / 掻痒症 / 脱水症 / 内部標準マーカー / 看護技術
Outline of Final Research Achievements

To establish the comprehensive assessment of skin local condition and systemic condition by skin blotting method which noninvasively extracts soluble proteins from skin tissue, we conducted the animal experiments and clinical studies. These results indicated that albumin extraction depends on the skin barrier function, and internal standard markers to normalize the extraction efficiency in skin blotting. Albumin and Annexin A2 are the promising internal standard markers. Furthermore, it is reported that skin blotting detecting NGFb and taurine reflects to itching and dehydration in older people.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (44 results)

All 2018 2017 2016 2015 Other

All Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 2 results) Presentation (24 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 2 results)

  • [Journal Article] Development of a distribution analysis method for metalloproteinase activity by combining wound blotting and zymography techniques: A pilot study in mice.2018

    • Author(s)
      Minematsu T, Nakagami G, Mugita Y, Tamai N, Sanada H.
    • Journal Title

      Journal of Nursing Science and Engineering.

      Volume: 5 Pages: 58-64

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reliability of the skin blotting method when used on the elderly.2018

    • Author(s)
      Koyano Y, Nakagami G, Minematsu T, Sanada H.
    • Journal Title

      International Wound Journal.

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of albumin using skin blotting as a measure of skin barrier function.2017

    • Author(s)
      Tamai N, Minematsu T, Tsunokuni S, Aso K, Higashimura S, Nakagami G, Sanada H.
    • Journal Title

      Journal of Nursing Science and Engineering.

      Volume: 4 Pages: 116-120

    • NAID

      130005875721

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biofilm detection by wound blotting can predict slough development in pressure ulcers: a retrospective observational study.2017

    • Author(s)
      Nakagami G, Schultz G, Gibson DJ, Phillips P, Kitamura A, Minematsu T, Miyagaki T, Hayashi A, Sasaki S, Sugama J, Sanada H.
    • Journal Title

      Wound Repair and Regeneration

      Volume: 25 Issue: 1 Pages: 131-138

    • DOI

      10.1111/wrr.12505

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Relationship of skin hardness of the temporal region to nutritional status.2017

    • Author(s)
      Uehata Y, Yoshida, Yabunaka K, Minematsu T, Tamai N, Sugama J, Sanada H.
    • Journal Title

      Journal of Nursing Science and Engineering

      Volume: 4 Pages: 49-57

    • NAID

      130006406569

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of epithelialization using the wound blotting method based on the distribution of transforming growth factor β: a pilot study.2016

    • Author(s)
      Kitamura A, Nakagami G, Minematsu T, Miyagaki T, Sasaki S, Sanada H.
    • Journal Title

      Journal of Japanese Society Wound, Ostomy, and Continence Management

      Volume: 20 Pages: 341-348

    • NAID

      130008032028

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased level of tumor necrosis factor-alpha (TNF-α) on the skin of Japanese obese males: measured by quantitative skin blotting.2016

    • Author(s)
      Ogai K, Matsumoto M, Aoki M, Minematsu T, Kitamura K, Kobayashi M, Sanada H and Sugama J
    • Journal Title

      Int J Cosmet Sci.

      Volume: in press Issue: 5 Pages: 462-469

    • DOI

      10.1111/ics.12312

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Association between components of exudates and periwound moisture-associated dermatitis in breast cancer patients with malignant fungating wounds.2016

    • Author(s)
      Tamai N, Akase T, Minematsu T, Higashi K, Toida T, Igarashi K, Sanada H.
    • Journal Title

      Biol. Res. Nurs.

      Volume: 18 Issue: 2 Pages: 199-206

    • DOI

      10.1177/1099800415594452

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Compression-induced HIF-1 enhances thrombosis and PAI-1 expression in mouse skin.2015

    • Author(s)
      49)Kaneko M, Minematsu T, Yoshida M, Nishijima Y, Noguchi H, Ohta Y, Nakagami G, Mori T, Sanada H.
    • Journal Title

      Wound Repair and Regeneration

      Volume: 23 Issue: 5 Pages: 657-663

    • DOI

      10.1111/wrr.12312

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of healing progress of pressure ulcers by distribution analysis of protein markers on necrotic tissue: a retrospective cohort study.2015

    • Author(s)
      Kitamura A, Yoshida M, Minematsu T, Nakagami G, Iizaka S, Fujita H, Naito A, Takahashi K, Mori T, Sanada H
    • Journal Title

      Wound Repair and Regeneration

      Volume: 23 Issue: 5 Pages: 772-777

    • DOI

      10.1111/wrr.12316

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression profiles of the antioxidant enzymes gene (SOD1, CAT, GPX, and HMOX1) in the skin of UV-irradiated and obese mice.2015

    • Author(s)
      Nishijima Y, Ibuki A, Minematsu T, Sanada H
    • Journal Title

      Journal of Nursing Science and Engineering.

      Volume: 3 Pages: 13-20

    • NAID

      130006406635

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histopathology of incontinence-associated skin lesions: inner tissue damage due to invasion of proteolytic enzymes and bacteria in macerated rat skin.2015

    • Author(s)
      Mugita Y, Minematsu T, Huang L, Nakagami G, Kishi C, Ichikawa Y, Nagase T, Oe M, Noguchi H, Mori T, Abe M, Sugama J, Sanada H.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 9 Pages: e0138117-e0138117

    • DOI

      10.1371/journal.pone.0138117

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Skin blotting analysis of Interleukin 6 on the buttock of wheelchair basketball players.2018

    • Author(s)
      Minematsu T, Tamai N, Maeda T, Tsunokuni S, Nakagami G, Yabunaka K, Noguchi H. Sanada H.
    • Organizer
      The 20th Annual Meeting of the European Pressure Ulcer Advisory Panel
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Skin blotting as a measure of albumin and nerve growth factor β can predict presence pruritus among elderly population.2018

    • Author(s)
      Dianis WS, Minematsu T, M Yoshida, H Sanada.
    • Organizer
      21st East Asian Forum of Nursing Scholars & 11th International Nursing Conferences
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dermatological study on risk factors for pruritic skin: skin properties of elderly population.2018

    • Author(s)
      Dianis WS, Minematsu T, Yoshida M, Sanada H.
    • Organizer
      20th International Conference on Nursing and Clinical Practices
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A possible biomarker for lymphatic injury detected by skin blotting technique: an experimental study in pressure ulcer model mice.2018

    • Author(s)
      Nakagami G, Kimura N, Minematsu T, Sanada H.
    • Organizer
      8th International Lymphoedema Framework Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of internal standard markers in skin blotting: Identification of candidate genes by mRNA expression analysis in rat skin.2018

    • Author(s)
      Minematsu T, TamaiN, Nakagami G, Sanada H.
    • Organizer
      第27回日本創傷・オストミー・失禁管理学会学術集会.
    • Related Report
      2017 Annual Research Report
  • [Presentation] Prediction of healing in Category I pressure ulcers by skin blotting with a lymphangiogenesis marker, vascular endothelial growth factor C: A pilot study.2018

    • Author(s)
      Nakai A, Minematsu T, Tamai N, Sugama J, Yabunaka K, Urai T, Tabata K, Sanada H.
    • Organizer
      第27回日本創傷・オストミー・失禁管理学会学術集会.
    • Related Report
      2017 Annual Research Report
  • [Presentation] 皮膚タウリンの非侵襲的測定による高張性脱水の同定.2018

    • Author(s)
      東村志保,峰松健夫,玉井奈緒,阿部智子,真田弘美.
    • Organizer
      日本老年看護学会第23回学術集会.
    • Related Report
      2017 Annual Research Report
  • [Presentation] Interdisciplinary approachを用いた足白癬の早期発見方法と予防ケアの開発.2018

    • Author(s)
      21)竹原君江,大江真琴,野口博史,峰松健夫,福田真佑,大橋優美子,門脇孝,真田弘美.
    • Organizer
      第16回日本フットケア学会年次学術集会.
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Properties of pruritus among elderly residents of Panti Werdha, public nursing homes in Indonesia.2017

    • Author(s)
      Dianis WS, Minematsu T, Yoshida M, Abe M, Sanada H.
    • Organizer
      9th World Congress on Itch.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ブロッティング膜貼付によるMDCK単層培養の蛍光標識デキストラン透過性の変化:スキンブロッティング法の原理解明を目指して.2017

    • Author(s)
      峰松健夫,津國早苗,工藤遥,玉井奈緒,真田弘美.
    • Organizer
      第5回看護理工学会学術集会・第11回看護実践学会学術集会・国際リンパ浮腫フレームワークジャパン研究協議会第7回学術集会 合同学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] スキンブロッティング法によるレジスタンストレーニング後のクレアチンキナーゼの動態の検討.2017

    • Author(s)
      禰屋光男,山崎浩司,峰松健夫.
    • Organizer
      第72回日本体力医学会大会.
    • Related Report
      2017 Annual Research Report
  • [Presentation] 陰イオン交換膜を用いたSkin Blotting法による皮膚の有機浸透圧調整物質の抽出.2017

    • Author(s)
      東村志保,峰松健夫,玉井奈緒,真田弘美.
    • Organizer
      第5回看護理工学会学術集会・第11回看護実践学会学術集会・国際リンパ浮腫フレームワークジャパン研究協議会第7回学術集会 合同学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 褥瘡発生予測のための分子マーカーに基づいた局所組織反応の検出による直接的皮膚アセスメント方法の開発.2016

    • Author(s)
      木村奈緒,仲上豪二朗,峰松健夫,真田弘美.
    • Organizer
      第46回日本創傷治癒学会.
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Skin Blotting法にタンパク質のサイズ分離を導入する試み.2016

    • Author(s)
      大貝和裕,青木未来,小林正和,峰松健夫,真田弘美,須釜淳子.
    • Organizer
      第46回日本創傷治癒学会.
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 糖尿病足潰瘍予防に向けてのケラチナーゼの可視化による足白癬スクリーニングツールの開発.2016

    • Author(s)
      竹原君江,峰松健夫,大江真琴,野口博史,常深祐一郎,駒形和典,國江慶子,武村雪絵,真田弘美.
    • Organizer
      第46回日本創傷治癒学会.
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] スキンブロッティング法によるマウス背部皮膚における皮膚バリア機能の評価.2016

    • Author(s)
      峰松健夫,津國早苗,麻生くみ,東村志保,玉井奈緒,仲上豪二朗,真田弘美.
    • Organizer
      第4回看護理工学会.
    • Place of Presentation
      岩手県立大学滝沢キャンパス(岩手県滝沢市)
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Evaluation of skin barrier function by skin blotting.2016

    • Author(s)
      Minematsu T, Nakagami G, Kitamura A, Goto T, Sanada H.
    • Organizer
      World Union of Wound Healing Societies (WUWHS) 2016
    • Place of Presentation
      Florence (Italy)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 超音波検査法を用いて計測した側頭筋厚と側頭部の陥没との関連.2016

    • Author(s)
      上畑陽子,吉田美香子,峰松健夫,藪中幸一,真田弘美.
    • Organizer
      第58回日本老年医学会学術集会.
    • Place of Presentation
      石川県立音楽堂(石川県金沢市)
    • Year and Date
      2016-06-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] 創傷治癒過程におけるペルオキシダーゼ活性の経時的変化2015

    • Author(s)
      北村言,峰松健夫,池田真一,仲上豪二朗,真田弘美.
    • Organizer
      第45回日本創傷治癒学会
    • Place of Presentation
      JPタワーホール&カンファレンス(東京都千代田区)
    • Year and Date
      2015-11-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 早期新生児期における額部の皮膚バリア機能と炎症性サイトカイン発現との関連2015

    • Author(s)
      樋口幸,峰松健夫
    • Organizer
      第3回看護理工学会学術集会
    • Place of Presentation
      立命館大学朱雀キャンパス(京都府京都市)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] ケラチナーゼの活性に着目した足白癬スクリーニングツールの開発.2015

    • Author(s)
      竹原君江,峰松健夫,真田弘美,武村雪絵
    • Organizer
      第3回看護理工学会学術集会.
    • Place of Presentation
      立命館大学朱雀キャンパス(京都府京都市)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 側頭部の皮膚硬度と栄養状態の関連検討.2015

    • Author(s)
      上畑陽子,雨宮歩,吉田美香子,藪中幸一,峰松健夫,真田弘美
    • Organizer
      第3回看護理工学会学術集会
    • Place of Presentation
      立命館大学朱雀キャンパス(京都府京都市)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] スキンブロッティング法を利用した高強度運動時の局所的筋動態の評価.2015

    • Author(s)
      禰屋光男,峰松健夫,松林武生,中村真理子,池田真一,後藤大地,土肥美智子
    • Organizer
      第70回日本体力医学会.
    • Place of Presentation
      和歌山県民文化会館/ホテルアバローム紀の国(和歌山県和歌山市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 静脈性下腿潰瘍の滲出液中の神経成長因子およびS100A8/A9は創傷の痛みと関係する.2015

    • Author(s)
      後藤大地,玉井奈緒,仲上豪二朗,峰松健夫,北村言,内藤亜由美,広川雅之,下川千佐子,真田弘美.
    • Organizer
      第24回日本創傷・オストミー・失禁管理学会学術集会
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
  • [Book] 看護理工学2015

    • Author(s)
      真田弘美,森武俊
    • Total Pages
      234
    • Publisher
      東京大学出版会
    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京大学大学院医学系研究科社会連携講座スキンケアサイエンス

    • URL

      http://skincarescience.m.u-tokyo.ac.jp/index.html

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] 東京大学大学院医学系研究科老年看護学/創傷看護学分野

    • URL

      http://www.rounenkango.m.u-tokyo.ac.jp/

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 対象の皮膚バリア機能を評価するための方法及びキット2017

    • Inventor(s)
      真田弘美,峰松健夫,仲上豪二朗
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 浸透圧調整物質定着用アニオン性イオン交換膜および浸透圧調整物質検出キット2017

    • Inventor(s)
      峰松健夫,磯村武範,真田弘美
    • Industrial Property Rights Holder
      東京大学、株式会社トクヤマ
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-196598
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 対象の皮膚バリア機能を評価するための方法及びキット2016

    • Inventor(s)
      真田弘美,峰松健夫,仲上豪二朗
    • Industrial Property Rights Holder
      真田弘美,峰松健夫,仲上豪二朗
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-186144
    • Filing Date
      2016-09-23
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 4)A marker for predicting pressure ulcer development and a use thereof.2016

    • Inventor(s)
      真田弘美,仲上豪二朗,峰松健夫
    • Industrial Property Rights Holder
      真田弘美,仲上豪二朗,峰松健夫
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-01-14
    • Related Report
      2015 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 白癬菌を検出するための方法及びキット2015

    • Inventor(s)
      竹原君江,峰松健夫,真田弘美
    • Industrial Property Rights Holder
      竹原君江,峰松健夫,真田弘美
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-201258
    • Filing Date
      2015-10-09
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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