| Project/Area Number |
15H05223
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
本多 了 金沢大学, 環境デザイン学系, 准教授 (40422456)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIYAMA Masateru 山形大学, 農学部, 助教 (10802928)
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| Research Collaborator |
CHIEMCHAISRI Chart カセサート大学, 工学部, 准教授
CHIEMCHAISRI Wilai カセサート大学, 工学部, 准教授
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 薬剤耐性菌 / 下廃水処理 / 東南アジア / 遺伝子解析 / 耐性遺伝子 / 活性汚泥 / 次世代シーケンス解析 / 細菌群集解析 / 下排水処理 / 熱帯アジア / タイ王国 / 次世代シーケンス技術 / 次世代シークエンス / タイ |
|---|
| Outline of Final Research Achievements |
Bacteria, which play an important role in carrying and transferring antibiotic-resistance genes (ARGs) in activated sludge process, were separated by incubation of sludge samples from wastewater treatment plants in Bangkok, Thailand, in the presence of antibiotics at a high concentration, followed by identification using next-generation sequencing technique. Percentage of Enterobacteriaceae clearly increased when sludge samples from 3 plants treating municipal wastewater were incubated with amoxicillin, ampicillin or ciprofloxacin, which demonstrates the presence of resistant bacteria belonging to this family. Similar results were obtained for sludge samples from hospital and university wastewater treatment plants. Expression level of ARGs (qnrS and sul1) relative to the total bacteriological activity was higher in the treatment process than in the influent, although it was not significantly correlated with response to stresses such as increased concentration of oxygen or antibiotics.
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