Budget Amount *help |
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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Outline of Final Research Achievements |
At Ministry of Health of Thailand, we analyzed blood sample of dengue infected patients and succeeded to identify (1) the viral serotypes (1-4 type) on the virus infected cultured cells using fluorescent labeled antibodies. Then, (2) EDTA was added to the blood supernatant, and genomic RNA was identified after the viral RNA isolation, double stranded DNA amplification by PCR. (3)dengue virus specific IgM and IgG were detected by anti-IgM and anti-IgG on a plate and detected by ELISA method. Based on the above described method, viral gene and the protein complex was labeled by chemically-modified antibody. The complex was captured by peptide nucleic acid on the test line in the sequence specific manner. It allow us to identify the presence of dengue virus in the specimen.
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