Diversity and evolution of anaerobic/microaerophilic eukaryotic microorganisms

• Project/Area Number: 15H05231
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Biodiversity/Systematics
• Research Institution: University of Tsukuba
• Principal Investigator: HASHIMOTO Tetsuo 筑波大学, 生命環境系, 教授 (50208451)
• Co-Investigator(Kenkyū-buntansha): 石田 健一郎 筑波大学, 生命環境系, 教授 (30282198) ; 稲垣 祐司 筑波大学, 計算科学研究センター, 教授 (50387958)
• Co-Investigator(Renkei-kenkyūsha): HANZAWA Naoto 山形大学, 理学部, 教授 (40292411)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2016: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 嫌気環境 / 真核微生物 / ミトコンドリア関連オルガネラ / 縮退進化 / 代謝多様性

Outline of Final Research Achievements

Various eukaryotic organisms that lack canonical mitochondria with oxygen respiration but instead possess mitochondrion related organelles (MROs) are present in anaerobic/microaerophilic environments. MROs are considered to be reduced mitochondria resulted from an adaptation to such environments. However, very little is known about diversity and evolution of these organisms and their MROs. In this study in order to further understand the diversity of MRO-containing organisms, we isolate various novel eukaryotic microorganisms from anaerobic/microaerophilic environments in Palau, Malaysia, and Japan and characterized these organisms by morphological, molecular phylogenetic, and comparative transcriptome analyses.

Research Products

• [Int'l Joint Research] Dalhousie University(Canada)
• [Int'l Joint Research] Universiti Teknologi Malaysia(Malaysia)
• [Journal Article] Fates of Evolutionarily Distinct, Plastid- type Glyceraldehyde 3-phosphate Dehydrogenase Genes in Kareniacean Dinoflagellates (2018)
  • Author(s): Kamikawa, R., Yazaki, E., Tahara, M., Sakura, T., Matsuo, E., Nagamune, K., Hashimoto, T., Inagaki, Y.
  • Journal Title: J. Eukaryot. Microbiol. Volume: 印刷中 Issue: 5 Pages: 669-678
  • DOI: 10.1111/jeu.12512
  • Peer Reviewed / Open Access
• [Journal Article] The draft genome of Kipferlia bialata reveals reductive genome evolution in fornicate parasites (2018)
  • Author(s): Tanifuji G, Takabayashi S, Kume K, Takagi M, Nakayama T, Kamikawa R, Inagaki Y, Hashimoto T
  • Journal Title: PLOS ONE Volume: 13 Issue: 3 Pages: e0194487-e0194487
  • DOI: 10.1371/journal.pone.0194487
  • NAID: 120007134280
  • Peer Reviewed / Open Access
• [Journal Article] Organelles that illuminate the origins of Trichomonas hydrogenosomes and Giardia mitosomes (2017)
  • Author(s): Leger M, Kolisko M, Ryoma Kamikawa, Stairs CW, Kume K, Cepicka I, Silberman JD, Andersson JO, Xu F, Yabuki A, Zhang Q, Takishita K, Inagaki Y, Simpson AGB, Hashimoto T, Roger AJ
  • Journal Title: Nature Ecology and Evolution Volume: 1 Issue: 4 Pages: 0092-0092
  • DOI: 10.1038/s41559-017-0092
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A Non-photosynthetic Diatom Reveals Early Steps of Reductive Evolution in Plastids (2017)
  • Author(s): Kamikawa R, Moog D, Zauner S, Tanifuji G, Ishida K, Miyashita H, Mayama S, Hashimoto T, Maier UG, Archibald JM, Inagaki Y
  • Journal Title: Molecular Biology and Evolution Volume: 34 Issue: 9 Pages: 2355-2366
  • DOI: 10.1093/molbev/msx172
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Microbial Eukaryotes that Lack Sterols (2017)
  • Author(s): Takishita K, Chikaraishi Y, Tanifuji G, Ohkouchi N, Hashimoto T, Fujikura K, Roger AJ
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 64 Issue: 6 Pages: 897-900
  • DOI: 10.1111/jeu.12426
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Global Kinetoplastea phylogeny inferred from a large-scale multigene alignment including prarasitic species for better understanding transitions from a free-living to a parasitic lifestyle (2017)
  • Author(s): Yazaki E, Ishikawa S, Kume K, Kumagai A, Kamaishi T, Tanifuji G, Hashimoto T, Inagaki Y
  • Journal Title: Gene & Genetic Systems Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mitochondrial genome of Palpitomonas bilix: derived genome structure and ancestral system for cytochrome c maturation (2016)
  • Author(s): Nishimura Y, Tanifuji G, Kamikawa R, Yabuki A, Hashimoto T, Inagaki Y
  • Journal Title: Genome Biology and Evolution Volume: 8 Issue: 10 Pages: 3090-3098
  • DOI: 10.1093/gbe/evw217
  • NAID: 120007129285
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 有中心粒太陽虫類におけるミトコンドリアゲノムの解読 (2018)
  • Author(s): 西村祐貴, 白鳥峻志, 石田健一郎, 橋本哲男, 稲垣 祐司, 大熊盛也
  • Organizer: 第12回ゲノム微生物学会
• [Presentation] Giardiaに近縁な自由生活性鞭毛虫Dysnectes brevisのMRO機能の推測 (2017)
  • Author(s): 井上 貴史, 谷藤 吾朗, 神川 龍馬, 久米 慶太郎, 稲垣 祐司, 橋本 哲男
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] A heterolobosean strain SRT213 and its mitochondrion-related organelle (2017)
  • Author(s): Yazaki Y, Shiratori T, Kume K, Hashimoto T, Ishida K, Inagaki Y
  • Organizer: ICOP 2017
  • Int'l Joint Research
• [Presentation] 新奇ヘテロロボサ生物SRT213における縮退的ミトコンドリア機能の推測 (2017)
  • Author(s): 矢崎裕規, 白鳥峻志, 久米慶太郎, 橋本哲男, 石田健一郎, 稲垣 祐司
  • Organizer: 日本進化学会第19回大会
• [Presentation] セスジアカムカデ消化管内寄生虫の網羅的mRNAデータに基づくアピコンプレクサの初期進化に関する一考察 (2017)
  • Author(s): 宮田 凌佑, 松尾 恵梨子, 中山 卓郎, 谷藤 吾朗, 千頭 康彦, 八畑 謙介, 橋本 哲男, 稲垣 祐司
  • Organizer: 日本進化学会第19回大会
• [Presentation] Mitochondrial genome of a protist branched at the base of Euglenozoa (2017)
  • Author(s): Yazaki Y, Shiratori T, Kume K, Hashimoto T, Ishida K, Inagaki Y
  • Organizer: Mitochondrial Genomics and Evolution, an SMBE Satellite Meeting
  • Int'l Joint Research
• [Presentation] ユーグレノゾア基部から分岐する新奇原生生物のミトコンドリアゲノム (2017)
  • Author(s): 矢崎裕規, 白鳥峻志, 久米慶太郎, 橋本哲男, 石田健一郎, 稲垣 祐司
  • Organizer: 第50回日本原生生物学会・第1回日本共生生物学会合同大会
• [Presentation] 陸生節足動物消化管から単離されたグレガリナ様寄生虫の系統学的位置と非光合成性色素体の探索 (2017)
  • Author(s): 宮田 凌佑, 松尾 恵梨子, 中山 卓郎, 谷藤 吾朗, 千頭 康彦, 八畑 謙介, 橋本 哲男, 稲垣 祐司
  • Organizer: 第50回日本原生生物学会・第1回日本共生生物学会合同大会
• [Presentation] A phylogenomic study placed a previously undescribed eukaryote, strain SRT308, at the base of the Euglenozoa clade (2016)
  • Author(s): Yazaki E, Shiratori T, Hashimoto T, Ishida K, Inagaki Y
  • Organizer: ICES 2016
  • Place of Presentation: Kyoto Prefectural University, Kyoto, Japan
  • Year and Date: 2016-09-10
  • Int'l Joint Research
• [Presentation] 真核生物進化の空白を埋める！分子系統解析が解き明かすプロティストの系統関係 (2016)
  • Author(s): 矢崎裕規, 白鳥峻志, 久米慶太郎, 石田健一郎, 橋本哲男, 稲垣祐司
  • Organizer: 日本進化学会第18回大会
  • Place of Presentation: 東京工業大学, 東京都・目黒区
  • Year and Date: 2016-08-25
  • Invited
• [Presentation] Characterization of strain SRT308; a new heterotrophic flagellate basal to Euglenozoa (2016)
  • Author(s): Shiratori T, Yazaki E, Inagaki Y, Hashimoto T, Ishida K
  • Organizer: Protis-2016 Moscow Forum
  • Place of Presentation: Moscow, Russia
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] 嫌気性生物PAP020株のミトコンドリア様オルガネラの代謝機能の推測 (2016)
  • Author(s): 矢崎裕規, 白鳥峻志, 久米慶太郎, 橋本哲男, 石田健一郎, 稲垣祐司
  • Organizer: 第85回日本寄生虫学会
  • Place of Presentation: 宮崎市民プラザ, 宮崎県・宮崎市
  • Year and Date: 2016-03-19
• [Presentation] The draft genome of Kipferlia bialata reveals that the gain of function contributes the massive reductive evolution in Metamonada (2016)
  • Author(s): Tanifuji G, Takabayashi S, Kume k, Takagi M, Inagaki Y, Hashimoto T
  • Organizer: Protis-2016 Moscow Forum
  • Place of Presentation: Moscow, Russia
  • Int'l Joint Research
• [Presentation] Prediction and comparison of the metabolism of mitochondrion-related organelles in free-living fornicate organisms (2015)
  • Author(s): Kume K, Takabayashi S, Kamikawa R, Tanifuji G, Inagaki Y, Hashimoto T
  • Organizer: 2nd International Symposium on Matryoshka-type Evolution of Eukaryotic Cells
  • Place of Presentation: University of Tsukuba, Tsukuba, Ibaraki, Japan
  • Year and Date: 2015-09-30
  • Int'l Joint Research
• [Presentation] Strain PAP020, a novel anaerobic microeukaryote branching at the base of Fornicata (2015)
  • Author(s): Yazaki Y, Shiratori, T, Kume K, Ishikawa SA, Ishida K, Hashimoto T, Inagaki Y
  • Organizer: VII ECOP-ISOP Joint Meeting 2015
  • Place of Presentation: Sevilla, Spain
  • Year and Date: 2015-09-05
  • Int'l Joint Research
• [Presentation] トランスクリプトームデータを基盤とした大規模分子系統解析による新奇嫌気性真核微生物PAP020株の系統的位置の解明 (2015)
  • Author(s): 矢崎裕規, 白鳥隆志, 久米慶太郎, 橋本哲男, 石田健一郎, 稲垣祐司
  • Organizer: 日本進化学会第17回大会
  • Place of Presentation: 中央大学, 東京都・文京区
  • Year and Date: 2015-08-20
• [Presentation] フォルニカータ＋パラバサリア生物群内でのミトコンドリア縮退進化 (2015)
  • Author(s): 髙林舜, 久米慶太郎, 神川龍馬, 谷藤吾朗, 稲垣祐司, 橋本哲男
  • Organizer: 第75回日本寄生虫学会東日本支部大会
  • Place of Presentation: 順天堂大学, 東京都・文京区