Isolation of endophytic fungi from rain forest in Indonesia and its utilization for remediation of post opencast mining land

• Project/Area Number: 15H05246
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Plant nutrition/Soil science
• Research Institution: Yamagata University
• Principal Investigator: Tawaraya Keitaro 山形大学, 農学部, 教授 (70179919)
• Co-Investigator(Kenkyū-buntansha): 塩野 義人 山形大学, 農学部, 教授 (80361278)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: エンドファイト / 菌類 / 熱帯雨林 / インドネシア / 土壌 / 二次代謝産物 / 森林土壌 / エポキシシクロへキセノン / 修復 / 接種 / 天然林 / 生理活性物質 / 培地

Outline of Final Research Achievements

Endophytic fungi (EPF) were isolated from forest soil and their function was clarified in order to remediate post opencast mining land in Indonesia. Thirty-three EFP were isolated by trap culture and those isolates increased growth of Brassica campestris andParaserianthes falcataria. Degree of increase was affected by nutrient concentration in media. More thant 10 new compounds were identified from 6 EPF. These results suggest that EPF can be used for remediation of post opencast mining land.

Academic Significance and Societal Importance of the Research Achievements

エンドファイト菌類を用いることにより露天掘り鉱山跡地に修復することが可能となり、インドネシアの森林破壊、土壌侵食、生物多様性の喪失、土壌や地下水の汚染などの環境問題を解決することができる。

Research Products

• [Int'l Joint Research] 環境林業省(インドネシア)
• [Journal Article] Isolation of endophytic fungi from tropical forest in Indonesia (2018)
  • Author(s): Ahdiar Fikri MAULANA, Maman TURJAMAN, Takumi SATO, Yasushi HASHIMOTO, Weiguo CHENG,Keitaro TAWARAYA
  • Journal Title: Symbiosis Volume: 76 Issue: 2 Pages: 151-162
  • DOI: 10.1007/s13199-018-0542-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] New isocoumarins, naphthoquinones, and a cleistanthane-type diterpene from Nectria pseudotrichia 120-1NP (2018)
  • Author(s): Ariefta, N. R., Kristiana, P., Aboshi, T., Murayama, T., Tawaraya, K., Koseki, T., Kurisawa, N., Kimura, K. & Shiono, Y
  • Journal Title: Fitoterapia Volume: 127 Pages: 356-361
  • DOI: 10.1016/j.fitote.2018.03.012
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Nutrient concentration in growth media affect relationship between host plant and fungal root endohytes (2016)
  • Author(s): Ahdiar Fikri Maulana, 程為国、俵谷圭太郎
  • Organizer: 日本土壌肥料学会２０１６年佐賀大会
  • Place of Presentation: 佐賀大学、佐賀市
  • Year and Date: 2016-09-20
• [Presentation] Secondary Metabolites Produced by Aspergillus stellatus T-5 and Fusarium solani T-13 Isolated from a Dead Branch (2016)
  • Author(s): Nanang Rudianto Ariefta, Tetsuya Murayama, Takuya Koseki, Yoshihito Shiono
  • Organizer: First International Symposium on Health-Care Technology 2016 in Yamagata
  • Place of Presentation: 山形大学、山形市
  • Int'l Joint Research
• [Presentation] New Secondary Metabolites Produced by Fungus Cylindrocarpon sp. SY-39 Isolated from a Driftwood (2016)
  • Author(s): Takuma Suzuki, Seiya Yoshida, Tetsuya Murayama, Takuya Koseki, Yoshihito Shiono
  • Organizer: First International Symposium on Health-Care Technology 2016 in Yamagata
  • Place of Presentation: 山形大学、山形市
  • Int'l Joint Research
• [Presentation] Isolation of endophytic fungi from forest soils in Indonesia and its effect on growth of Paraserianthes falcataria and Brassica campestris (2015)
  • Author(s): Ahdiar Fikri MAULANA, Weiguo CHENG, Keitaro TAWARAYA
  • Organizer: 日本土壌肥料学会２０１５年度京都大会
  • Place of Presentation: 京都市、京都大学
  • Year and Date: 2015-09-09