Studies on root associated microbes in ectomycorrhizal tree forests in NZ and Japan.

• Project/Area Number: 15H05249
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Forest science
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Chihiro 京都大学, 農学研究科, 教授 (60263133)
• Co-Investigator(Kenkyū-buntansha): 大澤 直哉 京都大学, 農学研究科, 准教授 (10221821) ; 門脇 浩明 京都大学, 森里海連環学教育研究ユニット, 特定助教 (30643548)
• Research Collaborator: Buchanan Peter K ; Johnston Peter R
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 菌根菌 / ナンキョクブナ / ブナ科 / Glutinomyces / 外生菌根 / 寄生 / Heterokryosis / 菌類 / きのこ / 根圏 / ラジアータマツ / アカマツ / シイ / 森林

Outline of Final Research Achievements

In this study, we identified ectomycorrhiza of black beech with exotic fungus amanita muscaria in New Zealand. We also investigated root-associated fungal communities in fagaceous roots in Japan. Hyaloscyphaceous fungi (Helotiales , Ascomycota) were dominantly detected at all four sites. This group of fungi was morphologically and phylogenetically investigated and a new genus Glutinomyces and four new species were proposed. We also elucidated their habitat in a mycorrhiza and parasexuality using molecular techniques.

Academic Significance and Societal Importance of the Research Achievements

ブナ科あるいはナンキョクブナ科は典型的な外生菌根性樹種であるが，植物根内ならびに菌根を含む根圏の微生物についてあまり知見が得られていない．本研究では，ニュジーランドで問題となっているナンキョクブナ林に侵入した外来菌根菌種（ベニテングタケなど）の菌根を調査するとともに，わが国の我が国ブナ科樹木の根圏ならびにマツタケシロの微生物の調査を行った．従来，樹木根圏に優占している可能性が指摘されていたHelotiales目菌について菌学的研究を行いそれらの分類，生態に新知見を加えた．

Research Products

• [Int'l Joint Research] Landcare Research CRI(New Zealand)
• [Int'l Joint Research] Landcare Research Institute(New Zealand)
• [Journal Article] Localization of helotialean fungi on ectomycorrhizae of Castanopsis cuspidata visualized by in situ hybridization. (2018)
  • Author(s): Nakamura N, Tanaka E, Tanaka C, Takeuchi-Kaneko Y
  • Journal Title: Mycorrhiza Volume: 28 Issue: 1 Pages: 17-18
  • DOI: 10.1007/s00572-017-0803-y
  • Peer Reviewed
• [Journal Article] Fungal Planet description sheets: 625-715 (2018)
  • Author(s): Crous PW et al (他113名）
  • Journal Title: Persoonia-Molecular Phylogeny and Evolution of Fungi Volume: 39 Pages: 270-467
  • DOI: 10.3767/persoonia.2017.39.11
  • Peer Reviewed
• [Journal Article] Seasonal changes in the content of the an (oxalato)aluminate complex, an antimicrobial substance of the structure in the shiro area “shiro” of Tricholoma matsutake, and the bacterial community (2017)
  • Author(s): Nishino K, Matsubara K, Tanaka C, Yaqmaguchi M, Fujita T, Yamada A, Hirai N
  • Journal Title: Mushroom Science and Biotechnology Volume: 25 Pages: 9-16
  • Peer Reviewed
• [Journal Article] The (oxalato)aluminate complex as an antimicrobial substance protecting the “shiro” of Tricholoma matsutake from soil micro-organisms (2017)
  • Author(s): Nishino, K.; Shiro, M.; Okura, R.; Oizumi, K.; Fujita, T.; Sasamori, T.; Tokitoh, N.; Yamada, A.; Tanaka, C.; Yamaguchi, M.; Hiradate, S.; Hirai, N.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 81 Issue: 1 Pages: 101-111
  • DOI: 10.1080/09168451.2016.1238298
  • Peer Reviewed / Acknowledgement Compliant