Epidemiological study of bovine tick-borne diseases and its establishment of diagnosis/preventive methods in Philippins

• Project/Area Number: 15H05264
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: TANAKA TETSUYA 鹿児島大学, 農水産獣医学域獣医学系, 教授 (00322842)
• Co-Investigator(Kenkyū-buntansha): 安藤 匡子 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (10466914) ; 正谷 達謄 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (70614072)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: マダニ / マダニ媒介性感染症 / アナプラズマ（リケッチア) / バベシア / タイレリア / フラビウイルス / アナプラズマ（リケッチア） / エーリキア / バベシア・タイレリア / アナプラズマ(リケッチア) / アナプラズマ(リケッチャ) / アナプラズマ(リケッチャ） / マダニ媒介性動物感染症 / ダニ脳炎ウイルス / ランガットウイルス

Outline of Final Research Achievements

Bovine tick-borne diseases including anaplasmosis and babesiosis causes great economic losses to animal industry worldwide and designated as an exotic infectious disease. Objective of the present study was to develop new gene diagnostic test against anaplasmosis and babesiosis to do molecular epidemiological survey on anaplasmosis and babesiosis in Philippines, and to contribute for strength of the quarantine system in Japan. As a result, new gene diagnostics was developed against bovine anaplasmosis and babesiosis. The epidemiological surveillance at the surround area of Manila in Philippines demonstrated high prevalence of tick-borne pathogens including anaplasmosis and babesiosis in these regions.

Academic Significance and Societal Importance of the Research Achievements

[本研究の学術的意義] 牛のマダニ媒介性感染症として代表的なアナプラズマ症ならびにバベシア症に対するPCR法やLAMP法などの遺伝子診断法が開発された。また、これらの診断法を用いたフィリピンマニラ周辺地域における牛のアナプラズマ症ならびにバベシア症の疫学調査を行ったところ、これらの地域でマダニ媒介性病原体の高い感染率が認められた。[本研究の社会的意義] 本研究で開発されたPCR法やLAMP法による診断技術が動物検疫体制の強化に貢献することが今後期待される。

Research Products

• [Int'l Joint Research] フィリピン大学・獣医学部/カビテ州立大学・獣医生命科学(フィリピン)
• [Int'l Joint Research] フィリピン大学・獣医学部/カビテ州立大学・獣医生命科学(フィリピン)
• [Int'l Joint Research] フィリピン大学・獣医学部/カビテ州立大学・獣医生命科学(フィリピン)
• [Int'l Joint Research] フィリピン大学・獣医学部/カビテ州立大学・獣医生命科学(フィリピン)
• [Journal Article] Molecular detection of tick-borne pathogens in canine population and Rhipicephalus sanguineus (sensu lato) ticks from southern Metro Manila and Laguna, Philippines (2018)
  • Author(s): Galay, R. L., Manalo, A. A., Dolores, S. L., Aguilar, I. P., Sandalo, K. A., Cruz, K., Divina, B., Andoh, M., Masatani, T. Tanaka, T.
  • Journal Title: Parasit. Vectors Volume: 11（1） Issue: 1 Pages: 643-643
  • DOI: 10.1186/s13071-018-3192-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] マダニ制圧へ向けた抗マダニワクチンの展望 (2018)
  • Author(s): 田仲哲也
  • Journal Title: 鹿児島県獣医師会会報 Volume: 61 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis (2017)
  • Author(s): Talactac, M. R., Yada, Y., Yoshii, K., Hernandez, E. P., Kusakisako, K., Maeda, H., Galay, R. L., Fujisaki, K., Mochizuki, M. and Tanaka, T.
  • Journal Title: Dev. Comp. Immunol. Volume: 68 Pages: 98-107
  • DOI: 10.1016/j.dci.2016.11.013
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Initial development of Babesia ovata in the tick midgut (2017)
  • Author(s): Maeda, H., Hatta, T., Alim, M. A., Tsubokawa, D., Mikami, F., Kusakisako, K., Matsubayashi, M., Umemiya-Shirafuji, R., Tsuji, N. and Tanaka, T.
  • Journal Title: Vet. Prasitol. Volume: 233 Pages: 39-42
  • DOI: 10.1016/j.vetpar.2016.11.020
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Detection and molecular characterization 1 of Babesia, Theileria, and Hepatozoon 2 species in hard ticks collected from Kagoshima, the southern region in Japan (2017)
  • Author(s): Masatani, T., Hayashi, K., Andoh, M., Tateno, M., Endo, Y., Asada, M., Kusakisako, K., Tanaka, T., Gokuden, M., Hozumi, N., Nakadohzono, F. and Matsuo, T.
  • Journal Title: Ticks Tick Borne Dis. Volume: In press
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Establishment of a novel tick-Babesia experimental infection mode (2016)
  • Author(s): Maeda, H., Hatta, T., Alim, M. A., Tsubokawa, D., Mikami, F., Matsubayashi, M., Miyoshi, T., Umemiya-Shirafuji, R., Kawazu, S., Igarashi, I., Mochizuki, M., Tsuji, N. and Tanaka, T.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 37039-37039
  • DOI: 10.1038/srep37039
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus. (2016)
  • Author(s): Talactac MR, Yoshii K, Maeda H, Kusakisako K, Hernandez EP, Tsuji N, Fujisaki K, Galay RL, Tanaka T, Mochizuki M.
  • Journal Title: Parasit Vectors Volume: 9 Issue: 1 Pages: 59-59
  • DOI: 10.1186/s13071-016-1344-5
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system. (2016)
  • Author(s): Masatani, T., Yoshihara, S., Matsubara, A., Gotoh, T., Takahashi, H., Tanaka, T., Andoh, M., Endo, Y. and Matsuo, T.
  • Journal Title: Acta Parasitol. Volume: 61 (2) Issue: 2 Pages: 419-424
  • DOI: 10.1515/ap-2016-0056
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media (2016)
  • Author(s): Kusakisako, K., Masatani, T., Yada Y., Talactac, M. R., Hernandez, E. P., Maeda, H., Mochizuki, M. and Tanaka, T.
  • Journal Title: Parasitol. Int. Volume: In press Issue: 5 Pages: 532-535
  • DOI: 10.1016/j.parint.2016.02.012
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Induction of gene silencing in Haemaphysalis longicornis ticks through immersion in double-stranded RNA (2016)
  • Author(s): Galay, R. L., Hernandez, E. P., Talactac, M. R., Maeda, H., Kusakisako, K., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
  • Journal Title: Ticks Tick Borne Dis. Volume: In press Issue: 5 Pages: 813-816
  • DOI: 10.1016/j.ttbdis.2016.03.018
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Molecular studies on tick-borne pathogens in large ruminants from selected areas of Calabarzon-neglected veterinary diseases in the Philippines (2018)
  • Author(s): Remil Linggatong Galay, Bea V. Ambita, Larry Xerxes B. Capuno Jr., DawnMaureen M. Chu, Carina R. Llaneta, Maria Karla Faye B. Monreal, Antero L. Armero III, Arianne Bel D. Baluyut, Princess DLP. Collera, Kristina Andrea C. Sandalo, Billy P. Divina, Tetsuya Tanaka
  • Organizer: 10th PSP Scientific Meeting
  • Int'l Joint Research
• [Presentation] Molecular detection of Rickettsia spp. and Coxiella burnetii in ruminantsand dogs from selected areas of Luzon Island, Philippines (2018)
  • Author(s): Remil Linggatong Galay, Bea V. Ambita, DawnMaureen M. Chu, Amdrew John M. Mariño, Irene Pearl M. Aguilar, Kristina Andrea C. Sandalo, Billy P. Divina, Masako Andoh, Tetsuya Tanaka
  • Organizer: 29th Meeting of the American Society for Rickettsiology,
  • Int'l Joint Research
• [Presentation] Molecular evidence of concurrent infections with tick-borne pathogens and Rickettsia in dogs from Luzon Island of the Philippine (2018)
  • Author(s): Remil Linggatong Galay, Anna Angelica L. Manalo, Sidney Lyndon D. Dolores, Kathlyn B. Cruz, Irene Pearl M. Aguilar, Kristina Andrea C. Sandalo, Billy P. Divina, Masako Andoh, Tatsunori Masatani, Tetsuya Tanaka
  • Organizer: 29th Internatipnal Congress of Parasitology (ICOPA 2018)
  • Int'l Joint Research
• [Presentation] Molecular detection of tick-borne pathogens in blood and ticks collected from cattle in Southern Luzon Island of the Philippines (2017)
  • Author(s): Remil Linggatong Galay, Larry Xerxes Capuno, Carina Lianeta, Kristina Andrea Sandalo, Melbourne Rio Talactac, Billy Divina, Masako Andoh, Tatsunori Masatani, Tetsuya Tanaka
  • Organizer: One Health 9th Tick and Tick-borne Pathogen Conference and 1st Asia Pacific Rikettsia Conference(TTP9-APRC1)
  • Int'l Joint Research
• [Presentation] The use of laboratory animals in tick rearing and research (2017)
  • Author(s): Remil Linggatong Galay, Hiroki Maeda, Tetsuya Tanaka
  • Organizer: 29th Philippine Association for Laboratory Animal Science (PALAS) Annual Scientific Conferenace and Workshop
  • Int'l Joint Research
• [Presentation] Experimental transmission of tick-borne encephalitis virus surrogate Langat virus by Haemaphysalis longicornis (2016)
  • Author(s): Melbourne Rio Talactac, Kentaro Yoshii, Emmauel Pacia Hernandez, Kodai Kusakisako, Hiroki Maeda, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka
  • Organizer: 第64回日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市)
  • Year and Date: 2016-10-23
• [Presentation] Babesia ovata原虫におけるマダニ中腸形態へのステージシフト誘導実験 (2016)
  • Author(s): 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林 誠, 三好猛晴, 白藤(梅宮)梨可, 辻 尚利, 田仲哲也
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（神奈川県・藤沢市)
  • Year and Date: 2016-09-06
• [Presentation] 鹿児島県各地で採取されたマダニにおけるマダニ媒介性原虫の分子疫学的解析 (2016)
  • Author(s): 正谷達謄, 林 慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 安藤匡子
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（神奈川県・藤沢市)
  • Year and Date: 2016-09-06
• [Presentation] Survival dynamics of Japan isolate Thogoto virus(Hl-Kamigamo-25) in Haemaphysalis longicornis (2016)
  • Author(s): Melbourne Rio Talactac, 好井健太郎, Emmanuel Pacia Hernandez, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（神奈川県・藤沢市)
  • Year and Date: 2016-09-06
• [Presentation] マウス寄生性コクシジウムEimeria krijgsmanni感染の再活性化 (2016)
  • Author(s): 水野真伸, 松林誠, 辻尾祐志, 正谷達謄, 田仲哲也, 松井利博, 松尾智英
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（神奈川県・藤沢市)
  • Year and Date: 2016-09-06
• [Presentation] マダニの自然免疫とバベシアとともに (2016)
  • Author(s): 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林 誠, 三好猛晴, 草木迫浩大, Remil Linggatong Galay, Melbourne Rio Talactac, 宮田 健, 白藤(梅宮)梨可, 河津信一郎, 五十嵐郁男, 望月雅美, 藤崎幸蔵, 辻 尚利, 田仲哲也
  • Organizer: 第24回分子寄生虫学ワークショップ第14回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広畜産大学（北海道・帯広市)
  • Year and Date: 2016-08-21
• [Presentation] 鹿児島県屋久島のヒゲナガチマダニから分離されたリケッチアHKT-1株の遺伝子解析 (2016)
  • Author(s): 安藤匡子, 本田俊郎, 御供田睦代, 藤田博己, 矢野泰弘, 正谷達謄, 田仲哲也, 松尾智英, 遠藤泰之, 安藤秀二, 川端寛樹
  • Organizer: 第68回日本衛生動物学会大会
  • Place of Presentation: 栃木県総合文化センター(栃木県・宇都宮市)
  • Year and Date: 2016-04-15
• [Presentation] 汎用的な細胞凍結保存液を用いたBabesia gibsoni原虫凍結保存法の検討およびその改良 (2016)
  • Author(s): 草木迫浩大, 正谷達謄, 屋田 友里花, Talactac Melbourne Rio, Hernandez Emmanuel Pacia, 前田大輝, 望月雅美, 田仲哲也
  • Organizer: 第85回日本寄生虫学会
  • Place of Presentation: 宮崎市民プラザ(宮崎市、宮崎)
  • Year and Date: 2016-03-19
• [Presentation] 鹿児島県各地で採取されたマダニにおけるTheileria, Babesia及びHepatozoonの分子疫学 (2016)
  • Author(s): 正谷達謄, 林 慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 関 まどか, 安藤匡子
  • Organizer: 第85回日本寄生虫学会
  • Place of Presentation: 宮崎市民プラザ(宮崎市、宮崎)
  • Year and Date: 2016-03-19
• [Presentation] フタトゲチマダニ体内における Babesia ovata移行動態について (2016)
  • Author(s): 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林 誠, 三好猛晴, 白藤(梅宮)梨可, 大久保和洋, 五十嵐郁男, 望月雅美, 藤崎幸蔵, 辻 尚利, 田仲哲也
  • Organizer: 第85回日本寄生虫学会
  • Place of Presentation: 宮崎市民プラザ(宮崎市、宮崎)
  • Year and Date: 2016-03-19
• [Presentation] 大分県久住高原の通年放牧牛におけるTheileria orientalisジェノタイプの年間変動 (2015)
  • Author(s): 正谷達謄, 吉原俊平, 松原敦子, 後藤貴文, 高橋秀之, 田仲哲也, 安藤匡子, 遠藤泰之, 松尾智英
  • Organizer: 第68回日本寄生虫学会南日本支部大会・第65回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 長崎大学(長崎市、長崎)
  • Year and Date: 2015-10-17
• [Presentation] 病原微生物感染に関与するマダニ生物活性分子について (2015)
  • Author(s): 田仲哲也
  • Organizer: 日本衛生動物学会・日本ダニ学会共催 公開シンポジウム
  • Place of Presentation: 法政大学(千代田区、東京都)
  • Year and Date: 2015-09-11
  • Invited
• [Presentation] ベクターマダニ体内におけるBabesia ovata増殖動態についての一考察 (2015)
  • Author(s): 前田大輝, 八田岳士, 松林 誠, 三好猛晴, 白藤(梅宮)梨可, 大久保和洋, 五十嵐郁男, 辻 尚利, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学(十和田市、青森)
  • Year and Date: 2015-09-07
• [Presentation] フタトゲチマダニ由来ロイシンリッチリピート保有タンパク質(HlLRR)の発現動態とバベシア原虫に及ぼす影響 (2015)
  • Author(s): 田仲哲也, 栗巣孔士, 前田大輝, 宮田 健, 草木迫浩大, Remil Linggatong Galay, Melbourne Rio Talactac, 望月雅美, 藤崎幸蔵
  • Organizer: 第23回SADI
  • Place of Presentation: 名取市文化会館(名取市、宮城)
  • Year and Date: 2015-06-26
• [Book] Ticks’ Antioxidant Complex: A Defense Stronghold and a Potential Target for Their Control (2017)
  • Author(s): Galay, R. L., Hernandez, E. P., Talactac, M. R., Kusakisako, K., Fujisaki, K. and Tanaka, T.
  • Total Pages: 285
  • Publisher: Nova Science Publishers, Inc.
• [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/V-Infection/sinkoukansen/index.html
• [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/V-infection/sinkoukansen/top.html