Epidemiological survey of human taeniasis/cysticercosis and animal cysticercosis and development of comprehensive detection method for soil-transmitted helminths.

• Project/Area Number: 15H05273
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Asahikawa Medical College
• Principal Investigator: Sako Yasuhito 旭川医科大学, 医学部, 教授 (40312459)
• Co-Investigator(Kenkyū-buntansha): 柳田 哲矢 山口大学, 共同獣医学部, 准教授 (40431837) ; 伊藤 亮 旭川医科大学, 医学部, 客員教授 (70054020) ; 岡本 宗裕 京都大学, 霊長類研究所, 教授 (70177096)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: テニア条虫 / 有鉤嚢虫 / 血清検査 / DNA検査 / 中間宿主 / 疫学調査 / 有鉤条虫 / 細頸嚢虫 / 蠕虫 / 検査法開発 / 流行調査 / 無鉤条虫 / 血清抗体検査 / DNA検出検査 / インドネシア / タイ

Outline of Final Research Achievements

Field survey of human taeniasis/cysticercosis and animal cysticercosis has been conducted in Bali, Indonesia. In human survey we could not find cysticercosis sero-positive case but could find six Taenia solium taeniasis patients. In animal survey, we found that pigs had been exposed to Taenia hydatigena infection. Additionally, we demonstrated that the antigen quality for the detection of anti-Taenia adult antibody of taeniasis patient was affected by the taenia adult worm used for antigen preparation. Furthermore, we developed a simple DNA detection test to differentiate human taenia parasites by loop-mediated isothermal amplification in combination with dot enzyme-linked immunosorbent assay.

Research Products

• [Int'l Joint Research] ウダヤナ大学(インドネシア)
• [Int'l Joint Research] ウダヤナ大学(インドネシア)
• [Int'l Joint Research] マヒドン大学(タイ)
• [Journal Article] Swine cysticercosis in the Karangasem district of Bali, Indonesia: An evaluation of serological screening methods (2016)
  • Author(s): Kadek Swastika, Nyoman Sadra Dharmawan, I. Ketut Suardita, I. Nengah Kepeng, Toni Wandra, 1, Yasuhito Sako, Munehiro Okamoto, Tetsuya Yanagida, Mizuki Sasaki, Patrick Giraudoux, Minoru Nakao, Takahiko Yoshida, Luh Putu Eka Diarthini, I. Made Sudarmaja, Ivan Elisabeth Purba, Christine M. Budke, Akira Ito
  • Journal Title: Acta Tropica Volume: 163 Pages: 46-53
  • DOI: 10.1016/j.actatropica.2016.07.022
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Simple identification of human Taenia species by multiplex loop-mediated isothermal amplification in combination with dot enzyme-linked immunosorbent assay. (2016)
  • Author(s): Nkouawa A, Sako Y, Okamoto M, Ito A
  • Journal Title: Am J Trop Med Hyg Volume: 94 Issue: 6 Pages: 1318-1323
  • DOI: 10.4269/ajtmh.15-0829
  • NAID: 120006325058
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Development of a simple identification of human Taenia species by using multiplex LAMP and dot-ELISA. (2015)
  • Author(s): Nkouawa Agathe、Ito Akira、Sako Yasuhito
  • Organizer: 64th American Society of Tropical Medicine & Hygiene Annual Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-10-25