Long-term effects on steroid hormone in children by dioxins and persistent organic pollutants in Vietnam

• Project/Area Number: 15H05285
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Hygiene and public health
• Research Institution: Kanazawa University
• Principal Investigator: KIDO TERUHIKO 金沢大学, 保健学系, 教授 (20167373)
• Co-Investigator(Kenkyū-buntansha): 中川 秀昭 金沢医科大学, 総合医学研究所, 教授 (00097437)
• Research Collaborator: OKAMOTO rie ; HONMA seijiro ; NISHIJO muneko ; NAKANO takeshi ; TAKASUGA takumi
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: ダイオキシン / 残留性有機汚染物質 / 小児 / ステロイドホルモン / 長期的影響 / ベトナム / コホート研究 / 残留性有機汚染物質(POPs) / ダイオキシン類

Outline of Final Research Achievements

Since 2002, the adverse health effects by herbicides containing dioxins sprayed during the Vietnamese War have been investigated and it was found that dioxins levels of lactating milk of mothers in heavily polluted area were significantly higher than those in the non-polluted area. Since 2008, steroid hormones of mothers and their children have been analyzed and significant positive correlation was shown between cortisol of serum and dioxin of breast milk in mothers.
During the research period on the children cohort, testosterone levels of 5 and 7 years old children in the sprayed area were significantly lower than those in the non-polluted area, respectively.
When cortisol levels of mothers were higher, it was found that 12 % of low birth body weight infants (less than 2,500 g) in heavily polluted area were 3 times higher than 4 % of those in the non-polluted area. From results, dioxins are strongly suspected the main cause of growth retardation of children in the polluted area.

Academic Significance and Societal Importance of the Research Achievements

我々のベトナムでの小児コホート研究では、7歳児の血中ステロイドホルモンは枯葉剤汚染地区の方が非汚染地区より有意に低く、その値は母乳中ダイオキシン（DXN）濃度と有意な負の相関を示した。
DXNの生物学的半減期が7-11年であることから、出生時のDXN暴露の影響が続いていると考えられる。
我が国では環境省が大規模な疫学調査「子どもの健康と環境に関する全国調査（エコチル調査）」を2011年より実施している。本研究はこの全国調査より先行しており、その成果は日本社会へ還元できるものと考えている。

Research Products

• [Int'l Joint Research] ハノイ医科大学(ベトナム)
• [Int'l Joint Research] ハノイ医科大学/ダイオキシン研究所/Phu Cat県立病院(ベトナム)
• [Journal Article] Androgen disruption by dioxin exposure in 5-year-old Vietnamese children: Decrease in serum testosterone level (2018)
  • Author(s): NTP Oanh, T Kido, S Honma, Y Oyama, LT Anh, HD Phuc, NH Viet, HD Manh, R Okamoto, H Nakagawa, SF Nakayama, DD Nhu, DV Tung, VV Chi, NH Minh, NV Toan
  • Journal Title: Science of The Total Environment Volume: 640;641 Pages: 466-474
  • DOI: 10.1016/j.scitotenv.2018.05.257
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A relationship in adrenal androgen levels between mothers and their (2017)
  • Author(s): Le Thai Anh, Teruhiko Kido, Seijiro Honma, Ho DungManh, Ikue Koike, Yuko Oyama,
  • Journal Title: Science of the Total Environment Volume: 607-608 Pages: 32-41
  • DOI: 10.1016/j.scitotenv.2017.06.264
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Inverse association of highly chlorinated dioxin congeners in maternal breast milk with dehydroepiandrosterone levels in three-year-old Vietnamese children. (2016)
  • Author(s): Kido T, Honma S, Nhu DD , Manh HD, Tung DV, Liang SX, Anh LT,Okamoto R, Maruzeni S,Nakagawa H, Hung NN, Son LK.
  • Journal Title: Sci Total Environ. Volume: 550 Pages: 248-255
  • DOI: 10.1016/j.scitotenv.2016.01.025
  • NAID: 120005743917
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Influence of dioxin exposure upon levels of prostate-specific antigen and steroid hormones in Vietnamese men (2016)
  • Author(s): Xian Liang Sun , Teruhiko Kido , Seijiro Honma , Rie Okamoto , Ho Dung Manh , Shoko Maruzeni , Muneko Nishijo , Hideaki Nakagawa , Takeshi Nakano , Eitetsu Koh , Takumi Takasuga , Dang Duc Nhu , Nguyen Ngoc Hung , Le Ke Son
  • Journal Title: Environmental Science and Pollution Research Volume: 23 Issue: 8 Pages: 7807-7813
  • DOI: 10.1007/s11356-015-5931-3
  • NAID: 120005743919
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Low birth weight of Vietnamese infants is related to their mother's dioxin and glucocorticoid levels. (2016)
  • Author(s): Van Tung D, Kido T, Honma S, Manh HD, Nhu DD, Okamoto R, Maruzeni S, Nishijo M, Nakagawa H, Ngoc PT, Van Toan N, Hung NN, Minh NH, Son LK.
  • Journal Title: Environ Sci Pollut Res Int. Volume: 23 Issue: 11 Pages: 10922-10929
  • DOI: 10.1007/s11356-016-6233-0
  • NAID: 120005743918
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reply to the letter to the editor "TCDD and birth weight of Vietnamese infants". (2016)
  • Author(s): Van Tung D, Kido T, Honma S, Manh HD, Nhu DD, Okamoto R, Maruzeni S, Nishijo M, Nakagawa H, Ngoc PT, Van Toan N, Hung NN, Minh NH, Son LK.
  • Journal Title: Environ Sci Pollut Res Int. Volume: 23 Issue: 21 Pages: 22218-22219
  • DOI: 10.1007/s11356-016-7518-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Urinary amino acid alterations in 3-year-old children with neurodevelopmental effects due to perinatal dioxin exposure in Vietnam: a nested case-control study for neurobiomarker discovery. (2015)
  • Author(s): Nishijo M, Tai PT, Anh NT, Nghi TN, Nakagawa H, Van Luong H, Anh TH, Morikawa Y, Waseda T, Kido T, Nishijo H
  • Journal Title: PLoS One Volume: 10 Issue: 1 Pages: e0116778-e0116778
  • DOI: 10.1371/journal.pone.0116778
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Levels of polychlorinated dibenzodioxins and polychlorinated dibenzofurans in breast milk samples from three dioxin-contaminated hotspots of Vietnam. (2015)
  • Author(s): Manh HD, Kido T, Tai PT, Okamoto R, Honma S, Liang SX, Anh le T, Maruzeni S, Nghi TN, Nishijo M, Nakagawa H, Nhu DD, Van Tung D, Hung NN, Son le K.
  • Journal Title: Sci Total Environ. Volume: 511 Pages: 416-422
  • DOI: 10.1016/j.scitotenv.2014.12.083
  • NAID: 120005537894
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dioxins and Nonortho PCBs in Breast Milk of Vietnamese MothersLiving in the Largest Hot Spot of Dioxin Contamination (2015)
  • Author(s): Tran Ngoc Nghi, Muneko Nishijo, Ho Dung Manh, Pham The Tai, Hoang Van Luong,Tran Hai Anh, Pham Ngoc Thao, Nguyen Viet Trung, Tomoo Waseda, Hideaki Nakagawa, Teruhiko Kido, Hisao Nishijo
  • Journal Title: Environmental Science and technology Volume: 49 Issue: 9 Pages: 5732-5742
  • DOI: 10.1021/es506211p
  • Peer Reviewed
• [Presentation] Steroid hormones disruption in 5-year-old children in two dioxin hotspot areas in Vietnam (2018)
  • Author(s): Kido T, Oanh NTP, Honma S, Oyama Y, Anh LT, Phuc HD, Nakagawa H, Nakayama SF, Nhu DD, Tung DV, Minh NH, Toan NV
  • Organizer: 38th International Symposium on Halogenated Persistent Organic Pollutants
  • Int'l Joint Research
• [Presentation] Two cohort studies on effects of dioxin levels in breast milk to steroid hormone concentrations in Vietnamese children (2018)
  • Author(s): Teruhiko Kido
  • Organizer: INTERNATIONAL CONFERENCE: ACHIEVEMENTS IN OVERCOMING CONSEQUENCES OF AGENT ORANGE/DIOXIN EFFECTS ON HUMAN BEING AND ENVIRONMENT
  • Int'l Joint Research / Invited
• [Presentation] ベトナムの２つのダイオキシン汚染地域における5歳児へのステロイドホルモン攪乱 (2018)
  • Author(s): Nguyen Thi Phuong Oanh, Teruhiko Kido, Seijiro Honma, Hoang Duc Phuc, Le Thai Anh, Yuko Oyama, Shoji Nakaiyama, Dang Duc Nhu, Ngo Van Toan, Nguyen Hung Minh
  • Organizer: 第88回日本衛生学会
• [Presentation] 7歳児のダイオキシン曝露後の成長発達とステロイドホルモンにおける性差への影響 (2018)
  • Author(s): 尾山木綿子, 城戸照彦, 本間誠次郎, Nguyen Thi Phuong Oanh,, Le Thai Anh, Hoang Duc Phuc,中山祥嗣, Dang Duc Nhu, Nguyen Hung Minh, Ngo Van Toan
  • Organizer: 第88回日本衛生学会
• [Presentation] The effect of dioxin on the growth and steroid hormone in serum of Vietnamese children (2017)
  • Author(s): Nguyen Thi Phuong Oanh, Teruhiko Kido, Seijiro Honma, Hoang Duc Phuc, Le Thai Anh, Yuko Oyama, Shoji Nakaiyama, Dang Duc Nhu, Ngo Van Toan, Nguyen Hung Minh
  • Organizer: 第87回日本衛生学会学術総会
  • Place of Presentation: フェニックス・シーガイア・リゾート（宮崎県宮崎市）
  • Year and Date: 2017-03-26
• [Presentation] ベトナムでのダイオキシン曝露と 5歳児のホルモン変化における性差の検討 (2017)
  • Author(s): 尾山木綿子, 城戸照彦, 本間誠次郎, Nguyen Thi Phuong Oanh,, Le Thai Anh, Hoang Duc Phuc,中山祥嗣, Dang Duc Nhu, Nguyen Hung Minh, Ngo Van Toan
  • Organizer: 第87回日本衛生学会学術総会
  • Place of Presentation: フェニックス・シーガイア・リゾート（宮崎県宮崎市）
  • Year and Date: 2017-03-26
• [Presentation] The effect of dioxin levels in breast milk on the steroid hormone concentrations in serum of seven-year-old Vietnamese children (2017)
  • Author(s): Kido T, Honma S, Kido Y, Oanh NTP, Phuc HD, Oyama Y, Anh LT, Viet NH, Nishijo M, Nakagawa H, Manh HD, Nhu DD, Tung DV, Tan NM, Hung NN, Toan NV, Minh NN, Son LK
  • Organizer: 37th International Symposium on Halogenated Persistent Organic Pollutants (POPs) - DIOXIN 2017
  • Int'l Joint Research
• [Presentation] The effect of dioxin levels in breast milk on the steroid hormone concentrations in serum of seven-year-old Vietnamese children (2016)
  • Author(s): Kido T, Honma S, Kido Y, Oanh NTP, Phuc HD, Oyama Y, Anh LT, Viet NH, Nishijo M, Nakagawa H, Manh HD, Nhu DD, Tung DV, Tan NM, Hung NN, Toan NV, Minh NN, Son LK
  • Organizer: 36th International Symposium on Halogenated Persistent Organic Pollutants (Dioxin 2016)
  • Place of Presentation: Florence, Italy
  • Year and Date: 2016-08-28
  • Int'l Joint Research
• [Presentation] Low DHEA levels in three-year-old children from Vietnamese mother-child pairs are associated with high-chloride dioxin isomers in maternal breast milk (2015)
  • Author(s): Kido T, Honma S, Manh HD, Anh LT, Nishijo M, Nakagawa H, Nhu DD, Tung DV, Hung NN, Son LK
  • Organizer: 35th International Symposium on Halogenated Persistent Organic Pollutants (Dioxin2015)
  • Place of Presentation: San Paulo - Brazil
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Remarks] 金沢大学 医薬保健研究域保健学系 公衆衛生・在宅看護学分野 城戸照彦
  • URL: http://square.umin.ac.jp/k-chiiki/kido/index.html