Epidemiological study on rotavirus diarrhea and a study on the mode of trasmisson

• Project/Area Number: 15H05286
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Hygiene and public health
• Research Institution: Nagasaki University
• Principal Investigator: ICHINOSE Yoshio 長崎大学, 熱帯医学研究所, 教授 (70176296)
• Co-Investigator(Kenkyū-buntansha): 長谷川 慎 長浜バイオ大学, バイオサイエンス学部, 教授 (10367899)
• Research Collaborator: Ernest Apondi Wandera ; Mohamed Monir Shah ; Erick Odoyo
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000); Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: ロタウイルス / ジェノタイピング / ワクチン効果 / 感染モード / ケニア / ウイルス検出システム / ノロウイルス / ウイルス粒子検出

Outline of Final Research Achievements

We have performed the epidemiological study of rotavirus diarrhea in Kenya in relation to countrywide introduction of a rotavirus vaccine (Rotarix vaccine) as well as the its impact to reduce the infections and the study of mode of transmission of rotavirus particles. Rotavirus vaccine reduced 50% of Rotavirus diarrhea in Kenya and a genotype G2P4 increase as a dominant genotype. We also could identify rotavirus particles out of 6.1% of the air samples collected by a metal mesh device by semi-nested PCR. It is suggested that rotavirus particles can spread via air in addition to the transmission via water and food.

Research Products

• [Journal Article] Impact of rotavirus vaccination on rotavirus hospitalization rates among a resource-limited rural population in Mbita, Western Kenya (2018)
  • Author(s): Ernest Apondi Wandera, Shah Mohammad1, Martin Bundi, James Nyangao, Amina Galata, Cyrus Kathiiko, Erick Odoyo, Sora Guyo, Gabriel Miring’u, Satoshi Komoto and Yoshio Ichinose
  • Journal Title: Tropical Medicine & International Health Volume: 2 Issue: 4 Pages: 201-211
  • DOI: 10.1111/tmi.13040
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Variation in Rotavirus Coverage by Sub-counties in Kenya (2017)
  • Author(s): Ernest A. Wandera1, Shah Mohammad1, John Ouko, James Yatitch, Koki Taniguchi and Yoshio Ichinose
  • Journal Title: Trop Med Health Volume: 45（9） Issue: 1 Pages: 223-239
  • DOI: 10.1186/s41182-017-0051-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of rotavirus in clinical specimens using an immunosensor prototype based on the photon burst counting technique (2017)
  • Author(s): Makoto Hasegawa, Ernest Apondi Wandera, Yuka Inoue, Nanami Kimura, Ryuzo Sasaki, Tamio Mizukami, Mohammad Monir Shah, Nobuaki Shirai, Osamu Takei, Hironori Shindo, and Yoshio Ichinose
  • Journal Title: Biomed. Opt. Express Volume: 8(7) Pages: 3383-3394
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Impact of Rotauirus and All-cause Gasteroenteritis in Peri-Urban Kenyan Children (2017)
  • Author(s): Ernest A. Wandera1, Mohammad1 S., Bundi M., Komoto S., Nyangao J., Kathihiko C., Odoyo E., Miring’u G., Taniguchi K. and Ichinose Y.
  • Journal Title: Vaccine Volume: 35 Pages: 5217-5223
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Burden of Rotavirus and Enteric Bacterial Pathogens among Children under 5 Years of Age Hospitalized with Diarrhea in Suburban and Rural Areas in Kenya (2017)
  • Author(s): Shah M, Odoyo E, Wandera EA, Bundi M, Miringu G, Guyo S, Kathiiko C, Galata A, Komoto S, Nyangao J, Karama M, Tsuji T, Taniguchi K, Morita K, Ichinose Y
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 70 Issue: 4 Pages: 442-447
  • DOI: 10.7883/yoken.JJID.2016.398
  • NAID: 130005851917
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Prevalence, Seasonal Variation and Antibiotic Resistance Pattern of Enteric Bacterial Pathogens Among Hospitalized Diarrheic Children in Suburban Regions of Central Kenya (2017)
  • Author(s): Mohammad Shah, Cyrus Kathiiko, Akihiro Wada, Erick Odoyo, Martin Bundi, Gabriel Miring’u, Sora Guyo, Mohamed Karama and Yoshio Ichinose
  • Journal Title: Tropical Medicine and Health Volume: 印刷中 Issue: 1
  • DOI: 10.1186/s41182-016-0038-1
  • NAID: 120006987592
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular Epidemiology of Rotavirus Gastroenteritis in Central Kenya Before Vaccine Introduction (2016)
  • Author(s): Ernest A. Wandera, Shah Mohammad, Satoshi Komoto, Yoshimasa Maeno, James Nyangao, Tomihiko Ide, Cyrus Kathiiko, Erick Odoyo, Takao Tsuji, Koki Taniguchi,* and Yoshio Ichinose
  • Journal Title: Journal of Medical Virology Volume: 89 Issue: 5 Pages: 809-817
  • DOI: 10.1002/jmv.24691
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Knowledge and practices regarding usage of Biological Safety Cabinets, (2016)
  • Author(s): Gabriel Miring’u, Martin Bundi, Ernest Wandera, Betty Muriithi, Amina Galata, Cyrus Kathiiko, Erick Odoyo, Sora Huka, Obed Ogumbo, John Ouko, Mohamed Karama, Mohammad Shah, Shingo Inoue, and Yoshio Ichinose.
  • Journal Title: Journal of ABSA International, Volume: 22 Issue: 1 Pages: 38-43
  • DOI: 10.1177/1535676016685790
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An outbreak of diarrhea in Mandera, Kenya due to Escherichia coli serogroup O-nontypable strain that had a coding gene for enteroaggregative E. coli heat-stable enterotoxin 1 (2016)
  • Author(s): Ochi, Sadayuki, Shah, Mohammad; Odoyo, Erick; Bundi, Martin; Miringu, Gabriel; Guyo, Sora; Wandera, Ernest; Kathiiko, Cyrus; Kariuki, Samuel; Karama, Mohammed; Tsuji, Takao; Yoshio Ichinose,
  • Journal Title: American Journal of Tropical Medicine and Hygiene, Volume: 96 Issue: 2 Pages: 457-464
  • DOI: 10.4269/ajtmh.16-0310
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] First Report of a Food-Borne Providencia alcalifaciens Outbreak in Kiambu, Kenya (2015)
  • Author(s): Mohammad M. Shah*, Erick Odoyo, Peter S. Larson, Ernest Apondi, Cyrus Kathiiko, Erick, Gabriel Miringu, Masahiro Nakashima, Yoshio Ichinose
  • Journal Title: The American Journal of Tropical Medicine and Hygiene Volume: Vol.93, No.3 Pages: 497-500
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evaluation of NUITM-KEMRI biosafety training program (2015)
  • Author(s): Inoue, S., Bundi, M., Miring’u, G., Muriithi, B., Ashur, S., Wamdera, E., Kathiiko, C., E. Odoyo., Galata, A., Huka, S., Karama, M., Kariuki, S., Horio, M., & Ichinose, Y
  • Journal Title: Journal of Biotechnology and Biosafety Volume: Vol.3, 4 Pages: 288-296
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Impact of Rotavirus Vaccination on Rotavirus and All-Cause Gastroenteritis in Central Kenya (2018)
  • Author(s): Ernest A. Wandela（研究協力者）
  • Organizer: 1st Africa-Asia Rotavirus Symposium
  • Int'l Joint Research
• [Presentation] Cholera outbreak caused by drug resistant Vibrio cholerae O1 strains in Kenya (2017)
  • Author(s): Mohammad Shah, Martin Bundi, Sora Guyo, Cyrus Kathiiko, Gabriel Miringu, Yoshio Ichinose
  • Organizer: the 13th Nagasaki-Singapore Medical Symposium/Leading Program International Symposium
  • Int'l Joint Research
• [Presentation] An outbreak of Diarrhea in Madera, Kenya Due to Escherichia coli Serogroup O-untypable strain that had a coding gene for Entero-aggregative coli heat-stable enterotoxin 1 (2017)
  • Author(s): Yoshio Ichinose, Sadayuki Ochi, Erick Odoyo, Martin Bundi, Gabriel Miringu, Sora Guyo, Samuel Kariuki, and Shah Mohammad
  • Organizer: 4th international Conference on Tropical Medicine Infectious Diseases & Public Health
  • Int'l Joint Research / Invited
• [Presentation] Early Impact of Rotavirus Vaccination in Rural Resource-Limited Setting in Kenya (2017)
  • Author(s): Shah Mohammad, Ernest Apondi Wandera, Martin Bundi, James Nyangao, Amina Galata, Cyrus Kathiiko, Erick Odoyo, Gabriel Miring’u, Satoshi Komoto, Yoshio Ichinose
  • Organizer: the 16th Awaji International Forum on Infection and Immunity
  • Int'l Joint Research
• [Presentation] Prevalence and virulence factors of Enteroaggregative Escherichia coli (EAEC) isolated from children admitted with diarrheal disease in Kiambu County, Kenya (2017)
  • Author(s): Erick Odoyo, Martin Bundi, Cyrus Kathiiko, Gabriel Miringu, Sora Guyo, Ernest Wandera, Mohamed Karama, Mohammad Shah, Yoshio Ichinose
  • Organizer: the 16th Awaji International Forum on Infection and Immunity
  • Int'l Joint Research
• [Presentation] An Outbreak of Diarrhea in Mandera, Kenya due to Escherichia coli Serogroup O-Nontypable strain that had a coding gene for Enteroaggegative E. coli heat-stable enterotoxin 1 (2017)
  • Author(s): Sadayuki Ochi1, Mohammad Shah, Erick Odoyo, Martin Bundi, Gabriel Miring’u, Sora Guyo, Ernest Wandera, Cyrus Kathiiko, Samuel Kariuki, Mohamed Karama, Takao Tsuji and Yoshio Ichinose
  • Organizer: The 3rd Africa International Biotechnology and Biomedical Conference
  • Int'l Joint Research
• [Presentation] Characterization of V. cholerae O1 strains isolated from cholera outbreaks in Kenya: 1975-2017 (2017)
  • Author(s): Martin Bundi, Mohammad Shah, Eric Odoyo, Cyrus Kathiiko, Ernest Wandera, Gabriel Miring’u, Sora Guyo, Amina Galata, Kouichi Morita and Yoshio Ichinose
  • Organizer: The 3rd Africa International Biotechnology and Biomedical Conference
  • Int'l Joint Research
• [Presentation] The emergence of Providencia alcalifaciens in Kenya, A Food Borne Pathogen Causes Diarrhea among Children in Kiambu, Kenya Between 2012-2013 (2016)
  • Author(s): Yoshio Ichinose, Erch Odoyo, Amina Galata and Mohammed Shah.
  • Organizer: BIT's 6th Annual Congress of Microbes-2016 (WCM-2016)
  • Place of Presentation: Nanjing International Youth Conference Hotel、南京、中国
  • Year and Date: 2016-11-16
  • Int'l Joint Research / Invited
• [Presentation] ケニアにおける小児下痢症の疾病負荷について (2016)
  • Author(s): Shah Mohammad、一瀬休生
  • Organizer: 日本熱帯医学会総会シンポジウム３ 熱帯地域における臨床疫学研究（S3-4）
  • Place of Presentation: 一橋大学(東京・国立市)
  • Year and Date: 2016-11-05
• [Presentation] Early Impact of Rotavirus Vaccination in Central Kenya (2016)
  • Author(s): Ernest A. Wandera, M. Shah, S. Komoto, J. Nyangao, C. Kathiiko, E. Odoyo, A. Galata, G. Miringu, K. Taniguchi and Y. Ichinose
  • Organizer: 12th International Rotavirus Symposium.
  • Place of Presentation: Pullman Albert Park Hotel, Melbourne, Australia
  • Year and Date: 2016-09-07
  • Int'l Joint Research
• [Presentation] Nagasaki University Kenya Research Station (2016)
  • Author(s): Yoshio Ichinose,
  • Organizer: Ticad6 pre-event
  • Place of Presentation: Forties Suites, ナイロビ、ケニア
  • Year and Date: 2016-08-25
  • Int'l Joint Research
• [Presentation] Separation and sensing of aerosol particles in Kenya using metal mesh sensor with periodic microstructures (2015)
  • Author(s): Yamamoto, K., E. Shirai, Y. Inoue, S. Kanba, T. Kondo, E. Wandera+, Y. Ichinose+, M. Hasegawa
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Seasonality of Rotavirus Gastroenteritis in Kenya: A Time-Series Analysis. (2015)
  • Author(s): Wandera E A, M Shah, S Komoto, C Kathiiko, E Odoyo, A Galata, J Nyangao, K Morita, K Taniguchi, M Hashizume, Y Ichinose
  • Organizer: 第63回日本ウイルス学会総会
  • Place of Presentation: 福岡国際会議場（福岡市）
  • Year and Date: 2015-11-22
• [Presentation] Separation and sensing of aerosol particles in Kenya using metal mesh sensor with periodic microstructures (2015)
  • Author(s): 5)Yamamoto, K., E. Shirai, Y. Inoue, S. Kanba, T. Kondo, E. Wandera+, Y. Ichinose+, M. Hasegawa
  • Organizer: IEEE sensors 2015
  • Place of Presentation: Busan（South Korea）
  • Year and Date: 2015-11-01
  • Int'l Joint Research
• [Presentation] Hospital-Based Surveillance of Rotavirus Gastroenteritis in Central Kenya, 2009-2014 (2015)
  • Author(s): 4)Wandera, E.A., M Shah, S Komoto, J Nyangao, C Kathiiko, E Odoyo, A Galata, G Miringu, K Morita, K Taniguchi, and Y Ichinose
  • Organizer: The 4th Medical and Veterinary Virus Research Symposium
  • Place of Presentation: ナイロビ（ケニア）
  • Year and Date: 2015-10-15
  • Int'l Joint Research
• [Presentation] Food poisoning caused by Bacillus pumilus in Kenya (2015)
  • Author(s): Mohammad Shah, Yoshio Ichinose:
  • Organizer: The 14th Awaji International Forum on Infection and Immunity,
  • Place of Presentation: 淡路夢舞台国際会議場（淡路市）
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Book] 日本防菌防黴学会誌 12月号 (2016)
  • Author(s): 一瀬休生
  • Total Pages: 7
  • Publisher: 日本防菌防黴学会