Construction of global standard molecular epidemiological method for identification of the children at risk for severe dental caries

Research Project

Project/Area Number	15H05300
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	海外学術
Research Field	Orthodontics/Pediatric dentistry
Research Institution	Osaka University
Principal Investigator	Nakano Kazuhiko 大阪大学, 歯学研究科, 教授 (00379083)
Co-Investigator(Kenkyū-buntansha)	仲周平岡山大学, 大学病院, 講師 (10589774) 仲野道代 (松本道代) 岡山大学, 医歯薬学総合研究科, 教授 (30359848) 高島由紀子岡山大学, 医歯薬学総合研究科, 助教 (30589768) 大川玲奈大阪大学, 歯学部附属病院, 講師 (80437384) 野村良太大阪大学, 歯学研究科, 准教授 (90437385) 藤田一世岡山大学, 医歯(薬)学総合研究科, 講師 (00437386)
Research Collaborator	LAPIRATTANAKUL Jinthana マヒドン大学, 歯学部, 准教授
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000) Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2015: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords	分子疫学的手法 / Streptococcus mutans / 乳酸桿菌 / タイ / 幼稚園 / 保育園 / う蝕 / 歯科健診 / ミュータンス菌 / リスク判定 / 小児 / リスク / 日本 / リスク特定
Outline of Final Research Achievements	Saliva specimens were taken from 3- and 4-year-old Thai children attending a kindergarten and nursery school in Bangkok, Thailand, from which Streptococcus mutans and Lactobacillus species were isolated using selective medium for each. Findings obtained from the S. mutans isolates provided new data to be added to a previously constructed multilocus sequence typing (MLST) scheme, which revealed the possibility of the correlation of a specific group of strains with clinical findings related to dental caries. As for the Lactobacillus species, Lactobacillus fermentus was shown to be associated with severe dental caries, thus several strains were classified using an MLST scheme, with novel sequence types revealed.