| Project/Area Number |
15H05331
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Narihiro Takashi 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (20421844)
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| Research Collaborator |
YAMADA Masayoshi 鹿児島工業高等専門学校, 都市環境デザイン工学科, 准教授 (80469593)
KURODA Kyohei 都城工業高等専門学校, 物質工学科, 助教 (50783213)
NOBU Masaru 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 研究員 (40805644)
LIU Wen-Tso イリノイ大学, 土木環境工学系, 教授
LEE Po-Heng 香港理工大学, 土木環境工学系, 助教
TANG Yueqin 四川大学, 環境工学系, 教授
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 微生物ゲノム / 廃水処理 / メタゲノム / アミノ酸 / アミノ酸分解 / 微生物 / ゲノム / 環境動態 / シントロフィー / メタン発酵 / 環境 |
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| Outline of Final Research Achievements |
Metagenomic analysis of a methanogenic microbial community in an anaerobic bioreactor treating amino acid-containing wastewater revealed synergistic metabolic network of syntrophic substrate-oxidizing bacteria, methanogens, and functionally unknown microorganisms. A laboratory-scale upflow anaerobic sludge blanket reactor fed with wastewater discharged from soy sauce processing manufactory was operated at 20°C. We successfully recovered metagenomic bins of dominant microbes in the reactor. Metabolic reconstruction suggested that dominant microorganisms perform fermentative and syntrophic degradation of amino acids and catabolic by-products facilitated by various energy conservation systems. Thus, diverse anaerobic organisms may unite to form a metabolic network to perform complete degradation of amino acids in the methanogenic microbiota.
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