Quantitaive evalution of dynamic regulation of cancer cell adhesion

• Project/Area Number: 15H05351
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Saitama University
• Principal Investigator: YOSHIKAWA Hiroshi 埼玉大学, 理工学研究科, 准教授 (50551173)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000); Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2015: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
• Keywords: がん / 緑茶カテキン / 細胞膜 / 反射干渉顕微法 / 力学計測 / 細胞接着 / 界面 / 超解像光学顕微法 / バイオメカニクス / 細胞骨格 / 膜 / 転移 / 光計測 / 細胞・組織 / 生物物理 / 生体材料 / 癌 / 応用光学・量子光工学 / 細胞運動 / 細胞力学 / バイオマテリアル / バイオテクノロジー / ポリフェノール / ハイドロゲル

Outline of Final Research Achievements

In this work, to quantitatively understand dynamic adhesion process of cancer cells, interface structures and mechanical properties of cancer cells have been characterized by using various optical/mechanical measurement techniques together with functional materials. The project gained mainly the following two achievements. (1) New experimental platforms for the quantitative evaluation of cancer cell adhesion was developed, which can be used to identify metastatic ability of cancer cells and to carry out drug screening. (2) Physico-chemical measurement revealed that green tea catechin with galloyl moiety can be physically adsorbed to phospholipid membranes, which leads to the significant stiffening of phospholipid membranes. This finding will be useful to design a new class of drugs for cancer therapy from mechanical viewpoints.

Research Products

• [Int'l Joint Research] ハイデルベルグ大学(ドイツ)
• [Int'l Joint Research] ハイデルベルグ大学(ドイツ)
• [Int'l Joint Research] Prof. Motomu Tanaka/Institute of Physical Chemistry/University of Heidelberg(ドイツ)
• [Journal Article] Structural change of nonionic surfactant self-assembling at electrochemically controlled HOPG/electrolyte interface (2017)
  • Author(s): Kobayashi Naritaka、Saitoh Hirokazu、Kawamura Ryuzo、Yoshikawa Hiroshi Y.、Nakabayashi Seiichiro
  • Journal Title: Journal of Electroanalytical Chemistry Volume: 799 Pages: 444-450
  • DOI: 10.1016/j.jelechem.2017.06.046
  • Peer Reviewed
• [Journal Article] Adsorption of galloyl catechin aggregates significantly modulates membrane mechanics in the absence of biochemical cues. (2017)
  • Author(s): Matsuzaki, T.; Ito, H.; Chevyreva, V.; Makky, A.; Kaufmann, S.; Okano, K.; Kobayashi, N.; Suganuma, M.; Nakabayashi, S.; Yoshikawa, H. Y.* & Tanaka, M.*
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 19 Issue: 30 Pages: 19937-19947
  • DOI: 10.1039/c7cp02771k
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Active control of growth mode and polymorph nucleation of organic molecules by femtosecond laser (2017)
  • Author(s): 丸山 美帆子、吉川 洋史
  • Journal Title: Journal of the Japanese Association for Crystal Growth Volume: 44 Issue: 3 Pages: n/a
  • DOI: 10.19009/jjacg.44-3-01
  • NAID: 130006206602
  • ISSN: 0385-6275, 2187-8366
  • Peer Reviewed
• [Journal Article] Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL (2017)
  • Author(s): Iida K, Sakai R, Yokoyama S, Kobayashi N, Togo S , Yoshikawa HY, Rawangkan A, Namiki K, Suganuma M.
  • Journal Title: Sci. Rep., Volume: ７ Issue: 1 Pages: 1-11
  • DOI: 10.1038/s41598-017-18120-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reflection Interference Microscopy~Noninvasive Nano-Imaging of Soft Interfaces~ (2017)
  • Author(s): 吉川 洋史、松崎 賢寿
  • Journal Title: Seibutsu Butsuri Volume: 57 Issue: 6 Pages: 318-322
  • DOI: 10.2142/biophys.57.318
  • NAID: 130006230293
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] Mechanical Response of Single Triacylglycerol Spherulites by Using Microcolloidal Probes (2017)
  • Author(s): Yoshikawa, H.Y.; Pink, D. A.; Acevedo, N. C.; Peyronel, F.; Marangoni, A. G.; Tanaka, M.
  • Journal Title: Chemistry Letters Volume: 46 Issue: 4 Pages: 599-601
  • DOI: 10.1246/cl.170014
  • NAID: 130005549063
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 細胞接着と培養場の硬さ～物理化学的観点からの生命現象の理解を目指して～ (2017)
  • Author(s): 松崎賢寿，吉川洋史，谷口英樹，武部貴則
  • Journal Title: 化学とマイクロ・ナノシステム Volume: Vol.16 No.1 Pages: 21-26
• [Journal Article] フェムト秒レーザーアブレーションを利用した結晶成長モードの能動的切り替えによるタンパク質結晶の成長促進 (2017)
  • Author(s): 吉川洋史, 丸山美帆子
  • Journal Title: 光学 Volume: Vol.46 No.6 Pages: 248-248
• [Journal Article] 肝臓オルガノイド×MedPhys Biology 物理視点から紐解くオルガノイドの自律形成メカニズム (2017)
  • Author(s): 吉川洋史, 東郷祥大，武部貴則
  • Journal Title: 実験医学 Volume: Vol.35 No.16 Pages: 2709-2713
• [Journal Article] 結晶を壊して大きくする!?──セレンディピティと異分野連携が生んだタンパク質結晶育成法 (2017)
  • Author(s): 吉川洋史、丸山美帆子
  • Journal Title: 化学 Volume: 72 Pages: 21-25
  • NAID: 40021190835
  • Acknowledgement Compliant
• [Journal Article] 人工細胞外基質の硬さと器官原基の自己組織化 (2017)
  • Author(s): 松崎賢寿、吉川洋史、谷口英樹、武部貴則
  • Journal Title: バイオマテリアルー生体材料ー Volume: 35 Pages: 58-61
• [Journal Article] Promotion of protein crystal growth by actively switching crystal growth mode via femtosecond laser ablation (2016)
  • Author(s): Y. Tominaga, M. Maruyama, M. Yoshimura, H. Koizumi, M. Tachibana, S. Sugiyama, H. Adachi, K. Tsukamoto, H. Matsumura, K. Takano, S. Murakami, T. Inoue, H. Y. Yoshikawa, Y. Mori
  • Journal Title: Nature Photonics Volume: 10 Issue: 11 Pages: 723-726
  • DOI: 10.1038/nphoton.2016.202
  • Peer Reviewed / Open Access
• [Journal Article] Biophysical Approach to Mechanisms of Cancer Prevention and Treatment with Green Tea Catechins (2016)
  • Author(s): Masami Suganuma, Atsushi Takahashi, Tatsuro Watanabe, Keisuke Iida,Takahisa Matsuzaki, Hiroshi Y. Yoshikawa, Hirota Fujiki
  • Journal Title: Molecules Volume: 21 Issue: 11 Pages: 5166-5166
  • DOI: 10.3390/molecules21111566
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Kinesin-driven active substrate giving stochastic mechanical stimuli to cells for characterization (2016)
  • Author(s): Ryuzo Kawamura, Daiki Uehara, Naritaka Kobayashi, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa
  • Journal Title: ACS Biomaterials Science & Engineering Volume: 2 Issue: 12 Pages: 2333-2338
  • DOI: 10.1021/acsbiomaterials.6b00538
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reverse Engineering Liver Buds Through Self-Driven Condensation And Organization Towards Medical Application (2016)
  • Author(s): Tadahiro Shinozawa, Hiroshi Y. Yoshikawa, Takanori Takebe
  • Journal Title: Developmental Bilogy Volume: 420 Issue: 2 Pages: 221-229
  • DOI: 10.1016/j.ydbio.2016.06.036
  • Peer Reviewed
• [Journal Article] Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: An experimental study on the anti-adhesion effect of green-tea catechins (2016)
  • Author(s): Rumi Sakamoto, Eisuke Kakinuma, Kentaro Masuda, Yuko Takeuchi, Kosaku Ito, Kentaro Iketaki, Takahisa Matsuzaki, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa, Hideaki Yamamoto, Yuko Sato, Takashi Tanii
  • Journal Title: In Vitro Cellular & Developmental Biology -Animal Volume: 52 Issue: 8 Pages: 799-805
  • DOI: 10.1007/s11626-016-0049-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy (2016)
  • Author(s): 4)T. Matsuzaki, K. Ito, K. Masuda, E. Kakinuma, R. Sakamoto, K. Iketaki, H. Yamamoto, M. Suganuma, N. Kobayashi, S. Nakabayashi, T. Tanii, H. Y. Yoshikawa
  • Journal Title: Journal of Physical Chemistry B Volume: 120 Issue: 7 Pages: 1221-1227
  • DOI: 10.1021/acs.jpcb.5b11870
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Live cell tracking of symmetry break in actin cytoskeleton triggered by abrupt changes in micromechanical environments (2015)
  • Author(s): S. Inoue, V. Frank, M. Hoerning, S. Kaufmann, H. Y. Yoshikawa, J. P. Madsen, A. L. Lewis, S. P. Armes and M. Tanaka
  • Journal Title: Biomater. Sci. Volume: 3 Issue: 12 Pages: 1539-1544
  • DOI: 10.1039/c5bm00205b
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] ヒト iPS 細胞由来肝臓オルガノイドを用いた炎症・線維化モデルの構築 In vitro Model for Inflammation and Fibrosis using Human iPSC-derived Liver Organoid (2018)
  • Author(s): 東郷 祥大、大内 梨江、篠澤 忠紘、小池 博之、吉川 洋史、武部 貴則
  • Organizer: 第65回 応用物理学会 春季学術講演会
  • Invited
• [Presentation] フェムト秒レーザーアブレーションで制御するタンパク質結晶の成長機構 (2018)
  • Author(s): 丸山美帆子、吉川洋史、吉村政志、森勇介
  • Organizer: 日本化学会 第98春季年会 (2018)
• [Presentation] DNA-PEG 脂質を介した細胞接着機構の解明ー細胞組織形成の自在制御に向けてー (2018)
  • Author(s): 東郷 祥大、佐藤 健、川村 隆三、小林 成貴、中林 誠一郎、寺村 裕治、吉川 洋史
  • Organizer: 日本化学会 第98春季年会 (2018)
• [Presentation] 液中周波数変調原子間力顕微鏡を用いた緑茶カテキンを含む脂質二重膜の分子分解能観察 (2017)
  • Author(s): 岡野 和希、小林 成貴、川村 隆三、中林 誠一郎、吉川 洋史
  • Organizer: 日本化学会第97回春季年会
  • Place of Presentation: 慶応義塾大学 日吉キャンパス，神奈川県横浜市
  • Year and Date: 2017-03-16
• [Presentation] Kinesin-driven active substrate for cells giving mechanical noise stimuli (2017)
  • Author(s): Ryuzo Kawamura, Daiki Uehara, Naritaka Kobayashi, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa
  • Organizer: 日本化学会第97回春季年会
  • Place of Presentation: 慶応大学 日吉キャンパス，神奈川県横浜市
  • Year and Date: 2017-03-16
• [Presentation] DNA hybridizationが細胞接着に与える影響の解明－細胞組織形成の人為的誘導に向けて－ (2017)
  • Author(s): 佐藤 健、 寺村裕治、 川村隆三、 小林成貴、中林誠一郎、吉川洋史
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2017-03-14
• [Presentation] フェムト秒レーザーアブレーションによる 有機結晶成長の時空間制御 (2017)
  • Author(s): 鈴木大希、中林誠一郎、吉川洋史
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2017-03-14
• [Presentation] がん細胞・幹細胞のバイオメカニクス (2017)
  • Author(s): 吉川 洋史
  • Organizer: 日本機械学会 2017年度年次大会
  • Invited
• [Presentation] フェムト秒レーザーアブレーションによる有機結晶の成長制御 (2017)
  • Author(s): 吉川 洋史
  • Organizer: 第78回応用物理学会 秋季学術講演会
  • Invited
• [Presentation] 集光レーザービームによるチューブリン凝集の時空間制御 (2017)
  • Author(s): 竹重 拓也、川村 隆三、呉 奇勲、中林 誠一郎、杉山 輝樹、吉川 洋史
  • Organizer: 第78回応用物理学会 秋季学術講演会
• [Presentation] レーザーアブレーションによる有機結晶成長の時空間制御２～レーザーエネルギー依存性～ (2017)
  • Author(s): 鈴木大希、中林誠一郎、吉川洋史
  • Organizer: 第78回応用物理学会 秋季学術講演会
• [Presentation] ヒトiPS細胞由来肝臓オルガノイドを用いた炎症・線維化モデルの構築 (2017)
  • Author(s): 東郷祥大、大内梨江、篠澤忠紘、小池博之、吉川洋史、武部貴則
  • Organizer: 第78回応用物理学会 秋季学術講演会
• [Presentation] Dynamics of Actin Cytoskeleton Remodeling Induced by Femtosecond Laser Ablation (2017)
  • Author(s): Kwokhoi Ng、Takeshige Takuya、Ryuzo Kawamura、 Seiichiro Nakabayashi、Yosuke Yoneyama、Fumihiko Hakuno、Shin-Ichiro Takahashi、Hiroshi Yoshikawa
  • Organizer: 第55回日本生物物理学会年会
• [Presentation] キネシンのパターン配置による微小管の異方的な動きの誘導 (2017)
  • Author(s): 廻谷圭祐、菊池 栞、小林成貴、吉川洋史、中林誠一郎、川村隆三
  • Organizer: 日本化学会秋季事業 第7回CSJ化学フェスタ2017
• [Presentation] ミニ肝臓を用いた脂肪酸蓄積による臓器構造・機能の時空間ダイナミクスの解明 (2017)
  • Author(s): 東郷祥大、大内梨江、篠澤忠紘、小池博之、吉川洋史、武部貴則
  • Organizer: 日本化学会秋季事業 第7回CSJ化学フェスタ2017
• [Presentation] フェムト秒レーザーアブレーションで制御するタンパク質結晶の成長機構 (2017)
  • Author(s): 丸山 美帆子、冨永勇佑、吉村政志、小泉晴比古、橘勝、杉山成、安達 宏昭、塚本勝男、松村浩由、高野和文、村上聡、井上豪、吉川 洋史、森勇介
  • Organizer: 第46回結晶成長国内会議
  • Invited
• [Presentation] Dynamics of cell-cell adhesion via DNA hybridization (2016)
  • Author(s): Ken Sato, Yuji Teramura, Ryuzo Kawamura, Naritaka Kobayashi, Seiichiro Nakabayashi, Hiroshi Yoshikawa
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場，茨城県つくば市
  • Year and Date: 2016-11-25
• [Presentation] Actin Cytoskeleton Remodeling Dynamics of Adherent Cells Under Mechanical Strain of Gelatin Substrate (2016)
  • Author(s): Kwokhoi Ng, Kentaro Iketaki, Ryuzo Kawamura, Seiichiro Nakabayashi, Yosuke Yoneyama, Fumihiko Hakuno, Shin-Ichiro Takahashi, Shinji Sugiura, Hiroshi Yoshikawa
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場，茨城県つくば市
  • Year and Date: 2016-11-25
• [Presentation] 規律的な組織形成に資する細胞‐外場間力学的相互作用の解明 (2016)
  • Author(s): 吉川洋史、川村隆三
  • Organizer: 日本バイオマテリアル学会シンポジウム2016
  • Place of Presentation: 福岡国際会議場，福岡県福岡市
  • Year and Date: 2016-11-21
  • Invited
• [Presentation] DNA hybridizationを介した細胞接着界面構造の光学計測～細胞組織化を制御する分子の論理的設計に向けて～ (2016)
  • Author(s): 佐藤健、寺村裕治、川村隆三、小林成貴、中林誠一郎、吉川洋史
  • Organizer: 第6回CSJ化学フェスタ2016
  • Place of Presentation: タワーホール船堀，東京都江戸川区
  • Year and Date: 2016-11-14
• [Presentation] Development of a new method for evaluating cell mechano-response to multidirectional and anisotropic mechanical strain by using photodegradable hydrogel (2016)
  • Author(s): 吉川洋史、池滝 憲太郎、柳川 史樹、川村 隆三、呉國愷、田中 泰誠、小嶋 勝、新井 健生、米山 鷹介、伯野 史彦、高橋 伸一郎、高木 俊之、金森 敏幸、中林 誠一郎、杉浦慎治
  • Organizer: 第67回コロイドおよび界面化学討論会
  • Place of Presentation: 北海道教育大学 旭川校，北海道旭川市
  • Year and Date: 2016-09-22
  • Invited
• [Presentation] Cross-linked microtubule network driven by kinesin as bio-active matter (2016)
  • Author(s): 川村隆三、上原大樹、小林成貴、中林誠一郎、吉川洋史
  • Organizer: 第67回コロイドおよび界面化学討論会
  • Place of Presentation: 北海道教育大学 旭川校，北海道旭川市
  • Year and Date: 2016-09-22
• [Presentation] Promotion of Protein Crystal Growth by Spatiotemporally Switching Crystal Growth Mode via Femtosecond Laser Ablation (2016)
  • Author(s): Hiroshi Yoshikawa, Yusuke Tominaga, Mihoko Maruyama, Masashi Yoshimura, Shigeru Sugiyama, Hiroaki Adachi, Katsuo Tsukamoto, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori
  • Organizer: The 18th International Conference on Crystal Growth and Epitaxy (ICCGE-18)
  • Place of Presentation: 名古屋国際会議場，愛知県名古屋市
  • Year and Date: 2016-08-07
  • Int'l Joint Research
• [Presentation] Development of a New Microscopy System Toward In-Situ Observation of Laser-Induced Crystal Growth Dynamics (2016)
  • Author(s): Daiki Suzuki, Seiichiro Nakabayashi, Hiroshi Yoshikawa
  • Organizer: The 18th International Conference on Crystal Growth and Epitaxy (ICCGE-18)
  • Place of Presentation: 名古屋国際会議場，愛知県名古屋市
  • Year and Date: 2016-08-07
  • Int'l Joint Research
• [Presentation] DNA－PEG資質を介した細胞接着ダイナミクスの解明 (2016)
  • Author(s): 佐藤健、寺村裕二、中林誠一郎、吉川洋史
  • Organizer: 日本化学会 第96春季年会
  • Place of Presentation: 同志社大学, 京都府, 京田辺市
  • Year and Date: 2016-03-24
• [Presentation] 脂質二分子膜の流動性がキネシン-微小管系の運動に与える影響の解明 (2016)
  • Author(s): 東郷祥大、川村隆三、小林成貴、中林誠一郎、吉川洋史
  • Organizer: 日本化学会 第96春季年会
  • Place of Presentation: 同志社大学, 京都府, 京田辺市
  • Year and Date: 2016-03-24
• [Presentation] Physical Interactions of Catechin Derivatives with Cell Membrane Models (2015)
  • Author(s): Takahisa Matsuzaki, Hiroaki Ito, Veronika Chevyreva, Ali Makky, Stefan Kaufmann, Masami Suganuma, Seiichiro Nakabayashi, Hiroshi Yoshikawa, Motomu Tanaka
  • Organizer: PACIFCHEM 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Mechanical Manipulation of Adherent Cells by Photodegradable Hydrogel (2015)
  • Author(s): Kentaro Iketaki, Fumiki Yanagawa, Ryuzo Kawamura, Seiichiro Nakabayashi, Toshiyuki Takagi, Shinji Sugiura, Toshiyuki Kanamori, Hiroshi Y. Yoshikawa
  • Organizer: PACIFCHEM 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Design of Mechanically Patterned Hydrogel for Directing Organ Bud Self-Formation (2015)
  • Author(s): Yuko Shimokawa, Hiroyuki Koike, Takahisa Matsuzaki, Masahiro Enomura, Masaki Kimura, Seiichiro Nakabayashi, Hiromitsu Nakauchi, Hideki Taniguchi, Takanori Takebe, Hiroshi Y. Yoshikawa
  • Organizer: PACIFCHEM 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Physical Studies of Cell-Substrate Mechanical Interactions for the Regulation of Cell Migration and Tissue Formation (2015)
  • Author(s): Hiroshi Y. Yoshikawa
  • Organizer: Hierarchical Dynamics in Soft Materials and Biological Matter
  • Place of Presentation: 京都大学, 京都府, 京都府
  • Year and Date: 2015-09-23
  • Int'l Joint Research / Invited
• [Presentation] Mechanical Study of Formation of Cell Aggregates on Hydrogel (2015)
  • Author(s): Yuko Shimokawa, Hiroyuki Koike, Takahisa Matsuzaki, Masahiro Enomura, Masaki Kimura, Seiichiro Nakabayashi, Hiromitsu Nakauchi, Hideki Taniguchi, Takanori Takebe, Hiroshi Y. Yoshikawa
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学, 石川県, 金沢市
  • Year and Date: 2015-09-13
• [Presentation] Application of photodegradable hydrogel to mechanical manipulation of adherent cells (2015)
  • Author(s): Kentaro Iketaki, Fumiki Yanagawa, Ryuzo Kawamura, Seiichiro Nakabayashi, Toshiyuki Takagi, Shinji Sugiura, Toshiyuki Kanamori, Hiroshi Y. Yoshikawa
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学, 石川県, 金沢市
  • Year and Date: 2015-09-13
• [Book] 「細胞社会学」の2.3節「細胞凝集塊制御技術」を執筆 (2017)
  • Author(s): 吉川洋史、武部貴則
  • Total Pages: 9
  • Publisher: コロナ社
• [Patent(Industrial Property Rights)] 細胞の弾性特性を解析する方法、解析システム、細胞の弾性特性を可視化する方法およびプログラム (2017)
  • Inventor(s): 小林成貴、吉川洋史、菅沼雅美、中林誠一郎
  • Industrial Property Rights Holder: 小林成貴、吉川洋史、菅沼雅美、中林誠一郎
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-179265
  • Filing Date: 2017