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Development of a bimodal near-infrared luminescent/TEM Platinum nanoprobes for in vivo imeging: Toward a better understanding of cancer metastasis

Research Project

Project/Area Number 15H05354
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionKure National College of Technology

Principal Investigator

Tanaka Shin-ichi  呉工業高等専門学校, 自然科学系分野, 准教授 (30455357)

Co-Investigator(Renkei-kenkyūsha) JIN Takashi  国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (80206367)
NIIOKA Hirohiko  大阪大学, データビリティフロンティア機構, 特任准教授 (70552074)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2015: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Keywords白金ナノクラスター / 癌転移機構 / in vivo イメージング / 近赤外蛍光 / 蛍光・電顕併用プローブ / 医療診断プローブ / 医療診断技術 / 生体1分子計測 / 蛍光顕微鏡 / 電子顕微鏡 / 生体分子プローブ / 近赤外蛍光性 / 1分子計測 / ナノバイオ / 癌診断 / 電子顕微鏡観察 / 生物物理 / 1分子計測 / 光化学 / 近赤外光 / ナノ粒子 / 生体イメージング
Outline of Final Research Achievements

Single molecule imaging technique reveals the molecular mechanism in living organisms and is powerful tool to provide for research in the biomedical field of cancer diagnosis and therapy. In this research, I developed the near-infrared emitting platinum nanoclusters, which possess the low cytotoxicity, highly photostability and high brightness, and applied them to single molecule in vivo imaging. The synthesized platinum nanoclusters exhibited the red emission (Em: 630 nm) and near-infrared emission (Em: 760 nm and 820 nm) and possessed about 1% quantum yield. Furthermore, I observed the scanning transmission electron microscope image of Pt nanoclusters to reveal the particle size and molecular structure. After conjugating with HER2 antibody, I labelled human breast cancer cell line (SK-BR-3) and observed the fluorescence on living cell. Finally, I conducted the in vivo imaging after injection of platinum nanoclusters and observed the fluorescence from cancer tissue in mice.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (7 results)

All 2018 2017 2015

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Study on Nanoparticle Sizing Using Fluorescent Polarization Method with DNA Fluorescent Probe2015

    • Author(s)
      Terutake Hayashi, Yuki Ishizaki, Masaki Michihata, Yasuhiro Takaya, Shin-ichi Tanaka
    • Journal Title

      International Journal of Automation Technology

      Volume: 9 Issue: 5 Pages: 534-540

    • DOI

      10.20965/ijat.2015.p0534

    • NAID

      130007674091

    • ISSN
      1881-7629, 1883-8022
    • Year and Date
      2015-09-05
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanoparticle sizing method based on fluorescence anisotropy analysis2015

    • Author(s)
      Terutake Hayashi, Yuki Ishizaki, Masaki Michihata, Yasuhiro Takaya, Shin-Ichi Tanaka
    • Journal Title

      Measurement

      Volume: 59 Pages: 382-388

    • DOI

      10.1016/j.measurement.2014.08.048

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 生体適合性金属ナノ材料の開発及びその医療応用に関する研究2018

    • Author(s)
      田中慎一
    • Organizer
      新技術説明会(ライフサイエンス分野)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 生体適合性金属ナノ材料の開発及びその医療応用に関する研究2018

    • Author(s)
      田中慎一
    • Organizer
      第17回OMIC事業推進セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Development of Near-Infrared-Emitting Platinum Nanoclusters aimed at in vivo Imaging and Biomedical Application2017

    • Author(s)
      Shin-Ichi Tanaka, Hirohiko Niioka
    • Organizer
      The International Nanophotonics Symposium (INP-2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Synthesis of biocompatible fluorescent metal nanoclusters and biomedical imaging2015

    • Author(s)
      Shin-ichi Tanaka
    • Organizer
      Pacifichem2015
    • Place of Presentation
      Hawaii Convention Center, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 白金ナノ粒子含有組成物、白金ナノ粒子、及びそれらの製造方法2015

    • Inventor(s)
      田中慎一
    • Industrial Property Rights Holder
      高専機構本部
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-114261
    • Filing Date
      2015-06-04
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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