Development of synthetic biological tools for modulating metabolic fates of cells

• Project/Area Number: 15H05371
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Chemical biology
• Research Institution: The University of Tokyo
• Principal Investigator: Komatsu Toru 東京大学, 大学院薬学系研究科(薬学部), 特任助教 (40599172)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000); Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: ケミカルバイオロジー / 創薬化学 / 酵素 / enzymomics / 生化学

Outline of Final Research Achievements

We tried to establish a novel synthetic biological systems to control metabolic fates of cells in time scales of seconds to minutes. For the purpose, we applied two independent approaches; (1) controlling the substrate or co-factor concentrations, or (2) controlling the enzymatic functions by small molecule-induced protein oligomerization. Through both approaches, we were able to confirm that the controlling metabolic activity by small molecules will be powerful techniques to understand cellular metabolism or controlling diseases caused by the metabolic malfunctions.

Research Products

• [Journal Article] Establishment of Molecular Design Strategy To Obtain Activatable Fluorescent Probes for Carboxypeptidases (2018)
  • Author(s): Kuriki Yugo、Kamiya Mako、Kubo Hidemasa、Komatsu Toru、Ueno Tasuku、Tachibana Ryo、Hayashi Kento、Hanaoka Kenjiro、Yamashita Suguru、Ishizawa Takeaki、Kokudo Norihiro、Urano Yasuteru
  • Journal Title: Journal of the American Chemical Society Volume: 140 Issue: 5 Pages: 1767-1773
  • DOI: 10.1021/jacs.7b11014
  • Peer Reviewed
• [Journal Article] Development of a Series of Practical Fluorescent Chemical Tools To Measure pH Values in Living Samples (2018)
  • Author(s): Takahashi Shodai、Kagami Yu、Hanaoka Kenjiro、Terai Takuya、Komatsu Toru、Ueno Tasuku、Uchiyama Masanobu、Koyama-Honda Ikuko、Mizushima Noboru、Taguchi Tomohiko、Arai Hiroyuki、Nagano Tetsuo、Urano Yasuteru
  • Journal Title: Journal of the American Chemical Society Volume: 印刷中 Issue: 18 Pages: 5925-5933
  • DOI: 10.1021/jacs.8b00277
  • Peer Reviewed
• [Journal Article] Fluorescence detection of serum albumin with a turnover-based sensor utilizing Kemp elimination reaction (2017)
  • Author(s): Sakamoto Shingo、Komatsu Toru、Ueno Tasuku、Hanaoka Kenjiro、Urano Yasuteru
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 27 Issue: 15 Pages: 3464-3467
  • DOI: 10.1016/j.bmcl.2017.05.076
  • Peer Reviewed
• [Journal Article] Development of an Azo-Based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy (2017)
  • Author(s): Piao Wen、Hanaoka Kenjiro、Fujisawa Tomotsumi、Takeuchi Satoshi、Komatsu Toru、Ueno Tasuku、Terai Takuya、Tahara Tahei、Nagano Tetsuo、Urano Yasuteru
  • Journal Title: J. Am. Chem. Soc. Volume: 139 Issue: 39 Pages: 13713-13719
  • DOI: 10.1021/jacs.7b05019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] An Activatable Photosensitizer Targeted to γ-Glutamyltranspeptidase (2017)
  • Author(s): Chiba Mayumi、Ichikawa Yuki、Kamiya Mako、Komatsu Toru、Ueno Tasuku、Hanaoka Kenjiro、Nagano Tetsuo、Lange Norbert、Urano Yasuteru
  • Journal Title: Angewandte Chemie International Edition Volume: 56 Issue: 35 Pages: 10418-10422
  • DOI: 10.1002/anie.201704793
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Potential of Enzymomics Methodologies to Characterize Disease-Related Protein Functions (2017)
  • Author(s): Toru Komatsu
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 65 Issue: 7 Pages: 605-610
  • DOI: 10.1248/cpb.c17-00144
  • NAID: 130006772511
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed
• [Journal Article] Development of Chemical Tools to Monitor and Control Isoaspartyl Peptide Methyltransferase Activity (2017)
  • Author(s): Yusuke Kimura, Toru Komatsu, Kouichi Yanagi, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Hirotatsu Kojima, Takayoshi Okabe, Tetsuo Nagano, Yasuteru Urano
  • Journal Title: Angew. Chem. Int. Ed. Volume: 56 Issue: 1 Pages: 153-157
  • DOI: 10.1002/anie.201608677
  • Peer Reviewed
• [Journal Article] Discovery and Mechanistic Characterization of Selective Inhibitors of H2S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase (3MST) Targeting Active-site Cysteine Persulfide (2017)
  • Author(s): K. Hanaoka, K. Sasakura, Y. Suwanai, S. Toma-Fukai, K. Shimamoto, Y. Takano, N. Shibuya, T. Terai, T. Komatsu, T. Ueno, Y. Ogasawara, Y. Tsuchiya, Y. Watanabe, H. Kimura, C. Wang, M. Uchiyama, H. Kojima, T. Okabe, Y. Urano, T. Shimizu, T. Nagano
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 40227-40227
  • DOI: 10.1038/srep40227
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development of a Reversible Fluorescent Probe for Reactive Sulfur Species, Sulfane Sulfur, and its Biological Application (2017)
  • Author(s): Takano Y, Hanaoka K, Shimamoto K, Miyamoto R, Komatsu T, Ueno T, Terai T, Kimura H, Nagano T, Urano Y
  • Journal Title: Chem Commun Volume: 53 Issue: 6 Pages: 1064-1067
  • DOI: 10.1039/c6cc08372b
  • Peer Reviewed / Open Access
• [Journal Article] Improving The Solubility of Artificial Ligands of Streptavidin to Enable More Practical Reversible Switching of Protein Localization in Cells (2017)
  • Author(s): Ryo Tachibana, Takuya Terai, Gaelle Boncompain, Shigeru Sugiyama, Nae Saito, Franck Perez and Yasuteru Urano
  • Journal Title: ChemBioChem Volume: 18 Issue: 4 Pages: 358-362
  • DOI: 10.1002/cbic.201600640
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates (2017)
  • Author(s): Narae Shin, Kenjiro Hanaoka, Wen Piao, Takuya Miyakawa, Tomotsumi Fujisawa, Satoshi Takeuchi, Shodai Takahashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Tahei Tahara, Masaru Tanokura, Tetsuo Nagano, Yasuteru Urano
  • Journal Title: ACS Chem. Biol. Volume: 12 Issue: 2 Pages: 558-563
  • DOI: 10.1021/acschembio.6b00852
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Enzyme-Loaded Polyion Complex Vesicles as in Vivo Nanoreactors Working Sustainably under the Blood Circulation: Characterization and Functional Evaluation (2017)
  • Author(s): Daiki Sueyoshi, Yasutaka Anraku, Toru Komatsu, Yasuteru Urano and Kazunori Kataoka
  • Journal Title: Biomacromolecules Volume: 18 Issue: 4 Pages: 1189-1196
  • DOI: 10.1021/acs.biomac.6b01870
  • Peer Reviewed
• [Journal Article] Discovery of Cell-type-specific and Disease-related Enzymatic Activity Changes via Global Evaluation of Peptide Metabolism (2017)
  • Author(s): Jun Onagi, Toru Komatsu, Yuki Ichihashi, Yugo Kuriki, Mako Kamiya, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Hiroyuki Matsuzaki, Keisuke Hata, Toshiaki Watanabe, Tetsuo Nagano, and Yasuteru Urano
  • Journal Title: J. Am. Chem. Soc. Volume: 139 Issue: 9 Pages: 3465-3472
  • DOI: 10.1021/jacs.6b11376
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Meeting Proceedings ICBS2016-Translating the Power of Chemical Biology to Clinical Advances (2017)
  • Author(s): Yugo Kuriki, Toru Komatsu, Peter D. Ycas, Sara K. Coulup, Erick J. Carlson, and William C. K. Pomerantz
  • Journal Title: ACS Chem. Biol. Volume: 12 Issue: 4 Pages: 869-877
  • DOI: 10.1021/acschembio.7b00205
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] ICBS and ECBS Chemical Biology Meeting 2015 - Let Them Come to Berlin (2016)
  • Author(s): Toru Komatsu and Satpal Virdee
  • Journal Title: ACS Chem. Biol., Volume: 11 Issue: 5 Pages: 1159-1166
  • DOI: 10.1021/acschembio.6b00268
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Protein-Coupled Fluorescent Probe To Visualize Potassium Ion Transition on Cellular Membranes (2016)
  • Author(s): Tomoya Hirata, Takuya Terai, Hisao Yamamura, Manabu Shimonishi, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Yuji Imaizumi, Tetsuo Nagano, and Yasuteru Urano
  • Journal Title: Analytical Chemistry Volume: 88 Issue: 5 Pages: 2693-2700
  • DOI: 10.1021/acs.analchem.5b03970
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Discovery of Pyruvylated Peptide-metabolizing Enzyme Using a Fluorescent Substrate-based Protein Discovery Technique (2016)
  • Author(s): Kentaro Yoshioka, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, Yasuteru Urano
  • Journal Title: Chemical Communications Volume: 52 Issue: 23 Pages: 4377-4380
  • DOI: 10.1039/c6cc00829a
  • Peer Reviewed
• [Journal Article] Detection of NAD(P)H-dependent enzyme activity by time-domain ratiometry of terbium luminescence (2016)
  • Author(s): Takuya Terai, Hiroki Ito, Kenjiro Hanaoka, Toru Komatsu, Tasuku Ueno, Tetsuo Nagano, Yasuteru Urano
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 26 Issue: 9 Pages: 2314-2317
  • DOI: 10.1016/j.bmcl.2016.03.038
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Unexpected photo-instability of 2,6-sulfonamide-substituted BODIPYs and its application to development of caged GABA (2016)
  • Author(s): Aoi Takeda, Toru Komatsu, Hiroshi Nomura, Naka Masamitsu, Norio Matsuki, Yuji Ikegaya, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano
  • Journal Title: ChemBioChem Volume: 17 Issue: 13 Pages: 1-9
  • DOI: 10.1002/cbic.201600097
  • Peer Reviewed
• [Journal Article] Development of Practical Red Fluorescent Probe for Cytoplasmic Calcium Ions with Greatly Improved Cell-membrane Permeability (2016)
  • Author(s): Kazuhisa Hirabayashi, Kenjiro Hanaoka, Takahiro Egawa, Chiaki Kobayashi, Shodai Takahashi, Toru Komatsua, Tasuku Ueno, Takuya Terai, Yuji Ikegaya, Tetsuo Nagano, Yasuteru Urano
  • Journal Title: Cell Calcium Volume: 60 Issue: 4 Pages: 256-265
  • DOI: 10.1016/j.ceca.2016.06.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rapid and sensitive detection of early esophageal squamous cell carcinoma with fluorescence probe targeting dipeptidylpeptidase IV. (2016)
  • Author(s): Onoyama H, Kamiya M, Kuriki Y, Komatsu T, Abe H, Tsuji Y, Yagi K, Yamagata Y, Aikou S, Nishida M, Mori K, Yamashita H, Fujishiro M, Nomura S, Shimizu N, Fukayama M, Koike K, Urano Y, Seto Y
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 26399-26399
  • DOI: 10.1038/srep26399
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Lung Inflammation-Related Elevation of Acylamino Acid Releasing Enzyme (APEH) Activity Using an Enzymomics Approach (2016)
  • Author(s): Yoko Takano, Kenjiro Hanaoka, Kazuhito Shimamoto, Ryo Miyamoto, Toru Komatsu, Tasuku Ueno, Takuya Terai, Hideo Kimura, Tetsuo Nagano, Yasuteru Urano
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 64 Issue: 11 Pages: 1533-1538
  • DOI: 10.1248/cpb.c16-00540
  • NAID: 130005268073
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Toward Total Synthesis of Cell Function: Reconstituting Cell Dynamics with Synthetic Biology (2016)
  • Author(s): Allen K. Kim, Robert DeRose, Tasuku Ueno, Benjamin Lin, Toru Komatsu, Hideki Nakamura, and Takanari Inoue
  • Journal Title: Sci. Signaling Volume: 9 Issue: 414
  • DOI: 10.1126/scisignal.aac4779
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of NAD (P) H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes (2015)
  • Author(s): Hiroki Ito, Takuya Terai, Kenjiro Hanaoka, Tasuku Ueno, Toru Komatsu, Tetsuo Nagano and Yasuteru Urano
  • Journal Title: Chem. Commun. Volume: 51 Issue: 39 Pages: 8319-8322
  • DOI: 10.1039/c5cc01613d
  • Description: in press
  • Peer Reviewed / Open Access
• [Journal Article] Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport (2015)
  • Author(s): Takuya Terai, Moe Kohno, Gaelle Boncompain, Shigeru Sugiyama, Nae Saito, Ryo Fujikake, Tasuku Ueno, Toru Komatsu, Kenjiro Hanaoka, Takayoshi Okabe, Yasuteru Urano, Franck Perez, and Tetsuo Nagano
  • Journal Title: J. Am. Chem. Soc. Volume: 137 Issue: 33 Pages: 10464-10467
  • DOI: 10.1021/jacs.5b05672
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evaluation of Enzymatic Activities in Living Systems with Small-molecular Fluorescent Substrate Probes (2015)
  • Author(s): Toru Komatsu and Yasuteru Urano
  • Journal Title: Analytical Sciences Volume: 31 Issue: 4 Pages: 257-265
  • DOI: 10.2116/analsci.31.257
  • NAID: 130005061526
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Gliotoxin Suppresses NF-kappaB Activation by Selectively Inhibiting Linear Ubiquitin Chain Assembly Complex (LUBAC) (2015)
  • Author(s): Hiroki Sakamoto, Shinichiro Egashira, Nae Saito, Takayoshi Kirisako, Simon Miller, Yoshiteru Sasaki, Tadahiko Matsumoto, Manabu Shimonishi, Toru Komatsu, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Hirotatsu Kojima, Takayoshi Okabe, Soichi Wakatsuki, Kazuhiro Iwai, Tetsuo Nagano
  • Journal Title: ACS Chemical Biology Volume: 10 Issue: 3 Pages: 675-681
  • DOI: 10.1021/cb500653y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] New Class of Bioluminogenic Probe Based on Bioluminescent Enzyme-Induced Electron Transfer: BioLeT. (2015)
  • Author(s): Takakura H, Kojima R, Kamiya M, Kobayashi E, Komatsu T, Ueno T, Terai T, Hanaoka K, Nagano T, Urano Y.
  • Journal Title: J Am Chem Soc. Volume: 137 Issue: 12 Pages: 4010-4013
  • DOI: 10.1021/ja511014w
  • Peer Reviewed
• [Journal Article] Development of a Series of Near-Infrared Dark Quenchers Based on Si-rhodamines and Their Application to Fluorescent Probes (2015)
  • Author(s): Takuya Myochin, Kenjiro Hanaoka, Shimpei Iwaki, Tasuku Ueno, Toru Komatsu, Takuya Terai, Tetsuo Nagano, and Yasuteru Urano
  • Journal Title: J. Am. Chem. Soc. Volume: １３７ Issue: 14 Pages: 4759-4765
  • DOI: 10.1021/jacs.5b00246
  • Peer Reviewed
• [Journal Article] Analysis of Chemical Equilibrium of Silicon-Substituted Fluorescein and Its Application to Develop a Scaffold for Red Fluorescent Probes (2015)
  • Author(s): K. Hirabayashi, K. Hanaoka, T. Takayanagi, Y. Toki, T. Egawa, M. Kamiya, T. Komatsu, T. Ueno, T. Terai, K. Yoshida, M. Uchiyama, T. Nagano, and Y. Urano
  • Journal Title: Analytical Chemistry Volume: 87(17) Issue: 17 Pages: 9061-9069
  • DOI: 10.1021/acs.analchem.5b02331
  • Peer Reviewed
• [Journal Article] Identification of tissue-restricted bio-reaction suitable for in vivo targeting by fluorescent substrate library-based enzyme discovery (2015)
  • Author(s): Kentaro Yoshioka, Toru Komatsu, Akihiro Nakada, Jun Onagi, Yugo Kuriki, Mitsuyasu Kawaguchi, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, and Yasuteru Urano
  • Journal Title: J. Am. Chem. Soc. Volume: 137 Issue: 38 Pages: 12187-12190
  • DOI: 10.1021/jacs.5b05884
  • Peer Reviewed
• [Journal Article] Development of a Sensitive Bioluminogenic Probe for Imaging Highly Reactive Oxygen Species in Living Rats (2015)
  • Author(s): Ryosuke Kojima, Hideo Takakura, Mako Kamiya, Eiji Kobayashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, and Yasuteru Urano
  • Journal Title: Angew. Chem. Int. Ed. Volume: 54 Issue: 49 Pages: 14768-14771
  • DOI: 10.1002/ange.201507530
  • Peer Reviewed
• [Presentation] Discovery of Disease-related Alternation of Enzymatic Functions with Enzymomics Approach (2018)
  • Author(s): Toru Komatsu, Jun Onagi, Yuki Ichikawa, Yasuteru Urano
  • Organizer: 日本化学会第98年春季大会
  • Invited
• [Presentation] Pathway-oriented screening法による解糖系酵素阻害剤の探索 (2018)
  • Author(s): 柳 光一 、小松徹、小島宏建、岡部隆義、長野哲雄、浦野泰照
  • Organizer: 日本薬学会第138年会
• [Presentation] マイクロデバイスを用いた単一酵素活性検出による病態診断法の開発 (2018)
  • Author(s): 坂本眞伍、小松徹、渡邉力也、張翼、野地博行、浦野泰照
  • Organizer: 日本薬学会第138年会
• [Presentation] 有機小分子蛍光プローブを用いた SAM高感度検出系の開発とその応用 (2018)
  • Author(s): 荻原州介、小松徹、浦野泰照
  • Organizer: 日本薬学会第138年会
• [Presentation] 生細胞系代謝スクリーニング法の開発による新規解糖系阻害剤群の探索 (2017)
  • Author(s): 柳光一，小松徹，浦野泰照
  • Organizer: 日本薬学会年会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-24
• [Presentation] ペプチドライブラリーを用いた疾患関連酵素の探索 (2017)
  • Author(s): 市橋裕樹，小名木淳，小松徹，浦野泰照
  • Organizer: 日本薬学会年会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-24
• [Presentation] マイクロデバイスを用いた一酵素活性の高精度解析による新規病態診断法の開発 (2017)
  • Author(s): 坂本眞伍，小松徹，張翼，渡邉力也，野地博行，浦野泰照
  • Organizer: 日本薬学会年会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-24
• [Presentation] 疾患関連酵素を探す、視る、操るケミカルバイオロジー研究ツールの開発 (2017)
  • Author(s): 小松徹
  • Organizer: 日本薬学会年会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-24
  • Invited
• [Presentation] Development of enzymomics approach to search for disease-related alternation of enzymatic functions (2017)
  • Author(s): Toru Komatsu, Jun Onagi, Yuki Ichihashi, Tetsuo Nagano and Yasuteru Urano
  • Organizer: International Chemical Biology Society Meeting 2017
  • Int'l Joint Research
• [Presentation] Development of enzymomicsapproach to search for disease-related alternation of enzymatic functions (2017)
  • Author(s): Toru Komatsu, Jun Onagi, Yuki Ichihashi, Tetsuo Nagano and Yasuteru Urano
  • Organizer: The Second International Symposium on Biofunctional Chemistry (ISBC 2017)
  • Int'l Joint Research
• [Presentation] Pathway-oriented screening法による解糖系酵素阻害剤の探索 (2017)
  • Author(s): 柳光一、小松徹、小島宏建、岡部隆義、長野哲雄、浦野泰照
  • Organizer: 日本ケミカルバイオロジー学会 第12回年会
• [Presentation] マイクロデバイスを用いた単一酵素活性検出による病態診断法の開発 (2017)
  • Author(s): 坂本眞伍、小松徹、渡邉力也、張翼、野地博行、浦野泰照
  • Organizer: 日本ケミカルバイオロジー学会 第12回年会
• [Presentation] ペプチドライブラリーを用いた酵素活性の網羅的解析法の確立と発展 (2017)
  • Author(s): 市橋裕樹、小名木淳、小松徹、神谷真子、松崎裕幸、畑啓介、渡邉聡明、浦野泰照
  • Organizer: 日本ケミカルバイオロジー学会 第12回年会
• [Presentation] 蛍光物質Boron dipyrromethene (BODIPY) 2,6位化学修飾新規蛍光群開発 (2017)
  • Author(s): 小松徹、Cherythu Nusaiba MADAPPURAM、浦野泰照
  • Organizer: 生体機能関連化学部会若手の会第29回サマースクール
• [Presentation] 小松徹、小名木淳、市橋裕樹、浦野泰照 (2017)
  • Author(s): Diced Electrophoresis Gel (DICE) 法を用いた癌細胞における機能異常酵素の探索
  • Organizer: 日本プロテオーム学会2017年大会
• [Presentation] ペプチドの「代謝」を視る技術を開発し新たな疾患関連タンパク質の機能を解明する (2017)
  • Author(s): 小松徹
  • Organizer: 第49回若手ペプチド夏の勉強会
  • Invited
• [Presentation] 酵素の動的機能の理解による疾患関連タンパク質の探索 (2017)
  • Author(s): 小松徹
  • Organizer: 第30回バイオメディカル分析科学シンポジウム(BMAS2017)
  • Invited
• [Presentation] Pathway-oriented screening法による解糖系酵素阻害剤の探索 (2017)
  • Author(s): 柳光一、小松徹、小島宏建、岡部隆義、長野哲雄、浦野泰照
  • Organizer: 第11回バイオ関連化学シンポジウム
• [Presentation] S-adenosyl-L-methionine(SAM)の高感度検出に基づく細胞内メチオニン代謝活性のハイスループット評価系の開発 (2017)
  • Author(s): 小松徹、荻原洲介、木村勇亮、浦野泰照
  • Organizer: 第35回メディシナルケミストリーシンポジウム
• [Presentation] ペプチドライブラリーを用いたenzymomicsプラットフォームの構築 (2017)
  • Author(s): 小松徹、小名木淳、市橋裕樹、浦野泰照
  • Organizer: 第68回日本電気泳動学会総会
• [Presentation] Pathway-oriented screening法による癌細胞の代謝制御化合物の探索 (2017)
  • Author(s): 小松徹、柳光一、荻原洲介、小島宏建、岡部隆義、長野哲雄、浦野泰照
  • Organizer: "ConBio2017(生命科学系合同年次大会) （第40回日本分子生物学会年会、第90回日本生化学大会）"
• [Presentation] 酵素の動的機能の理解に基づく 疾患関連タンパク質の 探索と機能評価 (2017)
  • Author(s): 小松徹
  • Organizer: "新学術領域「柔らかな分子系」第20回ワークショップ 「構造変化で操る分子の機能」"
  • Place of Presentation: 東京大学（東京都文京区）
  • Invited
• [Presentation] 疾患関連酵素を探す、視る、操るケミカルバイオロジー研究 (2017)
  • Author(s): 小松徹
  • Organizer: 第11回名市大頭脳循環セミナー
  • Place of Presentation: 名古屋市立大学（愛知県名古屋市）
  • Invited
• [Presentation] Discovery of Disease-related Protein Functions with High Throughput Global Evaluation of Enzymatic Activities (2016)
  • Author(s): Toru Komatsu, Jun Onagi, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano
  • Organizer: ICBS Meeting 2016
  • Place of Presentation: Univ. Wisconsin, Madison (Wisconsin, USA)
  • Year and Date: 2016-10-24
  • Int'l Joint Research
• [Presentation] Diced Electrophoresis Gel（DEG）アッセイ法を用いた癌細胞における活性異常酵素の探索 (2016)
  • Author(s): 小松徹，吉岡健太郎，花岡健二郎，長野哲雄，浦野泰照
  • Organizer: 第67回日本電気泳動学会総会
  • Place of Presentation: 釧路市観光国際交流センター（北海道釧路市）
  • Year and Date: 2016-08-26
• [Presentation] Diced Electrophoresis Gel（DEG）アッセイ法を用いた癌細胞における活性異常酵素の探索 (2016)
  • Author(s): 小名木淳，小松徹，花岡健二郎，長野哲雄，浦野泰照
  • Organizer: 生体機能関連化学部会若手の会 サマースクール
  • Place of Presentation: 西浦温泉ホテルたつき（愛知県蒲郡市）
  • Year and Date: 2016-07-15
• [Presentation] 細胞外代謝物の高感度検出による代謝過程制御化合物の探索 (2016)
  • Author(s): 柳光一，小松徹，浦野泰照
  • Organizer: 生体機能関連化学部会若手の会 サマースクール
  • Place of Presentation: 西浦温泉ホテルたつき（愛知県蒲郡市）
  • Year and Date: 2016-07-15
• [Presentation] 酵素活性の理解に基づく新規疾患関連タンパク質探索法の開発 (2016)
  • Author(s): 小松徹，小名木淳，柳光一，長野哲雄，浦野泰照
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜市（パシフィコ横浜）
  • Year and Date: 2016-03-29
  • Invited
• [Presentation] 新規癌イメージングプローブ開発を指向した蛍光プローブライブラリーの創製 (2016)
  • Author(s): 栗木優五、神谷真子、小松徹、居軒和也、高丸博之、阿部清一郎、斎藤豊、浦野泰照
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜市（パシフィコ横浜）
  • Year and Date: 2016-03-28
• [Presentation] 小分子代謝・輸送過程の新規評価系の構築 (2016)
  • Author(s): 柳光一、小松徹、浦野泰照
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜市（パシフィコ横浜）
  • Year and Date: 2016-03-28
• [Presentation] 有機小分子を使って 酵素を探す、酵素を視る (2016)
  • Author(s): 小松徹
  • Organizer: 第一回BRIGHTシンポジウム
  • Place of Presentation: 徳島大学（徳島県徳島市）
  • Invited
• [Presentation] 疾患関連タンパク質探索のための酵素活性の網羅的計測（enzymomics）技術の開発 (2016)
  • Author(s): 小松徹，小名木淳，花岡健二郎，長野哲雄，浦野泰照
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
• [Presentation] 疾患関連タンパク質探索のための酵素活性の網羅的計測（enzymomics）技術の開発 (2016)
  • Author(s): 小松徹，小名木淳，柳光一，花岡健二郎，浦野泰照
  • Organizer: 日本分析化学会第65年会
  • Place of Presentation: 北海道大学（北海道札幌市）
• [Presentation] 有機小分子蛍光プローブを用いた創薬標的酵素の探索と機能評価 (2016)
  • Author(s): 小松徹
  • Organizer: 第89回日本生化学会シンポジウム「アカデミア発創薬探索研究」
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Invited
• [Presentation] Diced electrophoresis gel assay for screening enzymes with specified activities (2015)
  • Author(s): Toru Komatsu, Jun Onagi, Akihiko Nakada, Tetsuo Nagano, Yasuteru Urano
  • Organizer: Pacifichem 2015
  • Place of Presentation: ホノルル（ハワイ）
  • Year and Date: 2015-12-18
  • Int'l Joint Research
• [Presentation] 有機小分子蛍光プローブを用いたペプチドisoaspartyl基のメチル化活性の高感度検出 (2015)
  • Author(s): 小松徹，木村勇亮，花岡健二郎，小島宏建，岡部隆義，長野哲雄，浦野泰照
  • Organizer: 第6回スクリーニング学研究会
  • Place of Presentation: 大宮市（ソニックシティ大宮）
  • Year and Date: 2015-11-27
• [Presentation] NADH検出蛍光プローブを用いたWarburg効果関連タンパク質阻害剤スクリーニング系 (2015)
  • Author(s): 柳光一，小松徹，浦野泰照
  • Organizer: 第6回スクリーニング学研究会
  • Place of Presentation: 大宮市（ソニックシティ大宮）
  • Year and Date: 2015-11-27
• [Presentation] 有機小分子蛍光プローブを用いたメチル基転移酵素活性の高感度検出法の開発と阻害剤探索 (2015)
  • Author(s): 小松徹，木村勇亮，花岡健二郎，小島宏建，岡部隆義，長野哲雄，浦野泰照
  • Organizer: 第32回メディシナルケミストリーシンポジウム
  • Place of Presentation: 千葉市（幕張国際研修センター）
  • Year and Date: 2015-11-25
• [Presentation] A novel cancer imaging technology by constructiong a fluorescent probe library (2015)
  • Author(s): Yugo Kuriki, Mako Kamiya, Haruna Onoyama, Toru Komatsu, Yasuyuki Seto,Yasuteru Urano
  • Organizer: 第52回ペプチド討論会
  • Place of Presentation: 平塚市（平塚市中央公民館）
  • Year and Date: 2015-11-16
• [Presentation] 有機小分子蛍光プローブを用いたペプチドisoaspartyl基のメチル化活性の高感度検出 (2015)
  • Author(s): 小松徹，木村勇亮，花岡健二郎，小島宏建，岡部隆義，長野哲雄，浦野泰照
  • Organizer: 第52回ペプチド討論会
  • Place of Presentation: 平塚市（平塚市中央公民館）
  • Year and Date: 2015-11-16
• [Presentation] Synthetic reconstitution of phagocytosis by chemically induced modification of cell-cell interaction (2015)
  • Author(s): Toru Komatsu
  • Organizer: ECBS & ICBS2015
  • Place of Presentation: ベルリン（ドイツ）
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Diced Electrophoresis Gel Assay（DEG）法を用いた新規創薬標的タンパク質の探索 (2015)
  • Author(s): 小松徹
  • Organizer: 第66回日本電気泳動学会総会
  • Place of Presentation: 大田区（東京工科大学蒲田キャンパス）
  • Year and Date: 2015-09-05
• [Presentation] "Synthetically Rerouting Phagocytosis by Rapidly Turning Inert Cells into “Eat You” Mode" (2015)
  • Author(s): Toru Komatsu
  • Organizer: New Biological Frontiers Illuminated by Molecular Sensors and Actuators
  • Place of Presentation: 台北（台湾）
  • Year and Date: 2015-06-29
  • Int'l Joint Research
• [Presentation] ペプチドライブラリーを用いた酵素活性の網羅的解析法の開発 (2015)
  • Author(s): 小名木淳、小松徹、浦野泰照
  • Organizer: 日本ケミカルバイオロジー学会第10回年会
  • Place of Presentation: 仙台市（東北大学川内キャンパス）
  • Year and Date: 2015-06-12
• [Presentation] 蛍光プローブライブラリー創製による新規迅速癌イメージングの実現 (2015)
  • Author(s): 栗木優五、神谷真子、小野山温那、小松徹、瀬戸泰之、浦野泰照
  • Organizer: 日本ケミカルバイオロジー学会第10回年会
  • Place of Presentation: 仙台市（東北大学川内キャンパス）
  • Year and Date: 2015-06-12
• [Presentation] 癌関連タンパク質Protein L-isoaspartyl methyltransferaseの活性評価法および活性制御化合物の開発 (2015)
  • Author(s): 木村勇亮、小松徹、小名木淳、小島宏建、岡部隆義、長野哲雄、浦野泰照
  • Organizer: 日本ケミカルバイオロジー学会第10回年会
  • Place of Presentation: 仙台市（東北大学川内キャンパス）
  • Year and Date: 2015-06-11
• [Presentation] 蛍光プローブライブラリーの創製による新規迅速癌イメージングの実現 (2015)
  • Author(s): 栗木優五、神谷真子、小野山温那、小松徹、瀬戸泰之、浦野泰照
  • Organizer: 第10回日本分子イメージング学会学術集会
  • Place of Presentation: 江戸川区（タワーホール船堀）
  • Year and Date: 2015-05-20
• [Book] 酵素のはたきを「見る」ケミカルバイオロジー研究／科学のとびら 60 「天然物の化学 - 魅力と展望」 (2016)
  • Author(s): 小松徹、浦野泰照
  • Total Pages: 200
  • Publisher: 株式会社東京化学同人
• [Patent(Industrial Property Rights)] マイクロデバイスへの適合性の高い酵素活性可視化蛍光プローブ (2017)
  • Inventor(s): 浦野 泰照，小松 徹，坂本 眞伍，野地 博行，渡邊 力也，張 翼
  • Industrial Property Rights Holder: 浦野 泰照，小松 徹，坂本 眞伍，野地 博行，渡邊 力也，張 翼
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-041149
  • Filing Date: 2017-03-03
• [Patent(Industrial Property Rights)] 細胞外代謝物を検出するための蛍光プローブ及び当該蛍光プローブを用いるスクリーニング方法 (2016)
  • Inventor(s): 浦野 泰照，小松 徹，柳 光一
  • Industrial Property Rights Holder: 浦野 泰照，小松 徹，柳 光一
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-11-22
  • Overseas