Comprehensive creation of antibody-like ligands for human proteomics
| Project/Area Number |
15H05372
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical biology
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| Research Institution | University of Yamanashi (2016-2017)
National Institute of Advanced Industrial Science and Technology (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
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| Keywords | プロテオミクス / HUPEX / ヒト腕型ロボット / 無細胞タンパク質合成系 / 部位特異的ビオチン化 / cDNAクローン / PUREシステム / in vitro virus / 無細胞翻訳系 / 環状Nアルキルペプチド / 汎用ヒト腕型ロボット / mRNAディスプレイ / cDNAディスプレイ / 抗体 / ペプトイド / DNLC-MS/MS / Nアルキルペプチド |
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| Outline of Final Research Achievements |
By integrating cyclic peptide ligand creation using PURE system and mRNA display with HUPEX to prepare immobilized human proteins from its encoding cDNA clones, we successfully created novel cyclic peptide ligands targeted to the human proteins in a full cell-free format. By combining the system with humanoid robot system, human protein-binding ligands created fully automatically were successfully applied to proteomic analysis.
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Report
(4 results)
Research Products
(24 results)
