| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Outline of Final Research Achievements |
Recently, the various types of genetically-encoded optogenetic tools using blue-light sensitive LOV2 domain from Phototropin1 have been developed and utilized in a wide range of areas including cell biology field. Here, we succeeded in developing a genetically-encoded light inducible CaMKII inhibitory peptide. Using this newly developed optogenetic tool, we found that the 1 min of CaMKII activation is sufficient for triggering structural plasticity of synapses in hippocampal neurons. Furthermore, using passive avoidance based memory test, we found that transient CaMKII activity is only required for fear memory formation in amygdala of mice.
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