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Neural circuit mechanisms underlying receptive field formation in higher-order visual cortical areas

Research Project

Project/Area Number 15H05375
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Brain biometrics
Research InstitutionNagoya University

Principal Investigator

Fumitaka Osakada  名古屋大学, 創薬科学研究科, 准教授 (60455334)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Keywords受容野 / DREADD / hM4Di / ウイルスベクター / 視覚弁別課題 / 経路選択的 / 多光子励起イメージング / 神経薬理学 / 2光子励起イメージング / 高次視覚野 / 視床 / 情報処理 / 視覚 / 視覚弁別行動 / 神経回路操作 / 薬理遺伝学 / 逆行性ウイルス / 2光子顕微鏡イメージング / 光遺伝学 / 視床核 / in vivoイメージング / ウイルス遺伝子工学 / 2光子顕微鏡 / カルシウムイメージング / 軸索 / 遺伝学 / Cre / AAV
Outline of Final Research Achievements

The aim of this project is to reveal how the receptive fields of higher-order visual cortical areas (HVAs) are shaped by the thalamus. We identified synaptic input to the V1 and each HVA by injecting retrograde viruses into each visual area of mice. Neurons of the lateral geniculate nucleus (LGN) neurons project to both the V1 and various HVAs, whereas neurons of the lateral posterior nucleus (LP) project to various HVAs, but not to the V1. We imaged visual responses from axon terminals of the LP or LGN neurons expressing GCaMP6 by 2-photon microscopy. In addition, we imaged visual responses from the V1 or HVA while manipulating activity of neurons in the LP or LGN with the Designer Receptors Exclusively Activated by Designer Drug (DREADD) system. From these results, we conclude that LP neurons play a pivotal role in formation of the receptive fields of HVA neurons in mice.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (30 results)

All 2018 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (20 results) (of which Int'l Joint Research: 2 results,  Invited: 13 results) Book (1 results)

  • [Journal Article] Toward understanding structure and function of neural circuits in the visual system2017

    • Author(s)
      恩田 将成、小坂田 文隆
    • Journal Title

      Folia Pharmacologica Japonica

      Volume: 149 Issue: 6 Pages: 274-280

    • DOI

      10.1254/fpj.149.274

    • NAID

      130006777530

    • ISSN
      0015-5691, 1347-8397
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of Cellular and Tissue-based Products for Retinal Regenerative Medicine.2017

    • Author(s)
      Osakada F.
    • Journal Title

      Yakugaku Zasshi.

      Volume: 137 Pages: 23-29

    • NAID

      130005180777

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Afferent Inputs to Neurotransmitter-Defined Cell Types in the Ventral Tegmental Area.2016

    • Author(s)
      Faget L, Osakada F, Duan J, Ressler R, Johnson AB, Proudfoot JA, Yoo JH, Callaway EM, Hnasko TS.
    • Journal Title

      Cell Rep.

      Volume: 15 Issue: 12 Pages: 2796-27808

    • DOI

      10.1016/j.celrep.2016.05.057

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Diverse Representations of Olfactory Information in Centrifugal Feedback Projections.2016

    • Author(s)
      Padmanabhan K, Osakada F, Tarabrina A, Kizer E, Callaway EM, Gage FH, Sejnowski TJ.
    • Journal Title

      J Neurosci.

      Volume: 36 Issue: 28 Pages: 7535-7545

    • DOI

      10.1523/jneurosci.3358-15.2016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Distributed and Mixed Information in Monosynaptic Inputs to Dopamine Neurons.2016

    • Author(s)
      Tian J, Huang R, Cohen JY, Osakada F, Kobak D, Machens CK, Callaway EM, Uchida N, Watabe-Uchida M.
    • Journal Title

      Neuron.

      Volume: 91 Issue: 6 Pages: 1374-1389

    • DOI

      10.1016/j.neuron.2016.08.018

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Distinct Hippocampal Pathways Mediate Dissociable Roles of Context in Memory Retrieval.2016

    • Author(s)
      Xu C, Krabbe S, Gründemann J, Botta P, Fadok JP, Osakada F, Saur D, Grewe BF, Schnitzer MJ, Callaway EM, Lüthi A.
    • Journal Title

      Cell.

      Volume: 167 Issue: 4 Pages: 961-972

    • DOI

      10.1016/j.cell.2016.09.051

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Visual system and inhibitory neural circuits in the mouse2016

    • Author(s)
      Suzuki T, Osakada F.
    • Journal Title

      Folia Pharmacologica Japonica

      Volume: 148 Issue: 3 Pages: 162-162

    • DOI

      10.1254/fpj.148.162

    • NAID

      130005261716

    • ISSN
      0015-5691, 1347-8397
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of novel therapies using human iPS cell-derived retinal pigment epithelia.2016

    • Author(s)
      Arakawa D, Ito A, Osakada F.
    • Journal Title

      Nihon Yakurigaku Zasshi.

      Volume: 148 Pages: 217-217

    • NAID

      130005598179

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Early Somatostatin Interneuron Connectivity Mediates the Maturation of Deep Layer Cortical Circuits.2016

    • Author(s)
      Tuncdemir SN, Wamsley B, Stam FJ, Osakada F, Goulding M, Callaway EM, Rudy B, Fishell G
    • Journal Title

      Neuron

      Volume: 89 Pages: 521-535

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 移植治療を目指したヒトiPS細胞から網膜色素上皮細胞への分化効率化2018

    • Author(s)
      伊藤 ありさ,葉 珂,荒川 大二朗,小坂田 文隆
    • Organizer
      日本再生医療学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 再生医療等製品開発に向けたヒトiPS細胞由来網膜色素上皮細胞シートの最適化2018

    • Author(s)
      葉 珂, 伊藤 ありさ, 小坂田 文隆
    • Organizer
      日本薬学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 長期の生理学実験および行動実験に適用可能なG欠損狂犬病ウイルスベクターの開発2018

    • Author(s)
      山口 真広、森本 菜央、小坂田 文隆
    • Organizer
      日本薬学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞種特異的な網膜神経回路の病態生理学的な役割を目指して2018

    • Author(s)
      小坂田 文隆
    • Organizer
      大阪大学蛋白研セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Viral and electrophysiological approaches to elucidate structure and function of retinal circuits2017

    • Author(s)
      小坂田 文隆
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Targeting enveloped viruses to two distinct populations for neural circuit analysis2017

    • Author(s)
      鈴木 俊章、坂口 綾菜、赤池 昭紀、小坂田 文隆
    • Organizer
      神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Targeting enveloped viruses to multiple, distinct populations for neural circuit analysis2017

    • Author(s)
      Toshiaki Suzuki, Fumitaka Osakada
    • Organizer
      The 48th NIPS International Symposium: Neural circuitry and plasticity underlying brain function
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Toward understanding the structure and function of neural circuits in the visual system2017

    • Author(s)
      Fumitaka Osakada
    • Organizer
      CEBST
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Viral and imaging approaches for linking neuronal connectivity to circuit function2017

    • Author(s)
      Fumitaka Osakada
    • Organizer
      遺伝研研究会「哺乳類脳の機能的神経回路の構築メカニズム」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 相互結合かつ共通入力を有するサブネットワークの新規解析技術2017

    • Author(s)
      小坂田 文隆
    • Organizer
      Conbio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 視覚機能の再生を目指した「再生医療等製品」の開発に向けて2017

    • Author(s)
      小坂田 文隆
    • Organizer
      第6回超異分野学会
    • Place of Presentation
      東京
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Neural Circuit Tracing with Rabies Viral Vectors for Neural Circuit Research2016

    • Author(s)
      Fumitaka Osakada
    • Organizer
      Viral Vector Technology for Neural Circuit andand Pathology Research
    • Place of Presentation
      岡崎
    • Year and Date
      2016-12-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Neural Circuit Tracing with Glycoprotein-Deleted Rabies Viruses2016

    • Author(s)
      Fumitaka Osakada
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] G遺伝子欠損狂犬病ウイルスを用いた新規神経回路解析法2016

    • Author(s)
      小坂田文隆
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      神奈川県、日本
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Neural circuit tracing with glycoprotein-deleted rabies viruses2016

    • Author(s)
      小坂田文隆
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      神奈川県、日本
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 網膜の神経回路を解析するウイルス遺伝子工学と電気生理学的手法2016

    • Author(s)
      小坂田 文隆
    • Organizer
      第31回東北眼疾患病態研究会
    • Place of Presentation
      仙台
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 「再生医療等製品」としてのiPS細胞由来網膜色素上皮細胞の可能性2016

    • Author(s)
      小坂田 文隆
    • Organizer
      生きた再生医療用材料の開発研究会
    • Place of Presentation
      名古屋
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 視覚再生を目指した新規治療開発2016

    • Author(s)
      小坂田 文隆
    • Organizer
      材料科学フロンティア研究院講演会
    • Place of Presentation
      名古屋
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Molecular and viral approaches to elucidate structure and function of neural circuits2015

    • Author(s)
      小坂田文隆
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会
    • Place of Presentation
      兵庫県、日本
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 神経回路の構造と機能を対応付ける狂犬病ウイルストレーシング法2015

    • Author(s)
      小坂田文隆
    • Organizer
      研究戦略ワークショップ「Strategy for Neuroscience 2015」
    • Place of Presentation
      東京都、日本
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Book] 臨床薬学テキストシリーズ バイオ医薬品と再生医療2016

    • Author(s)
      小坂田文隆、高橋政代
    • Total Pages
      283
    • Publisher
      中山書店
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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