Molecular electrochemical switching elements for bioimaging

• Project/Area Number: 15H05415
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Nano/Microsystems
• Research Institution: Tohoku University
• Principal Investigator: Ino Kosuke 東北大学, 工学研究科, 准教授 (00509739)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000); Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2015: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
• Keywords: 電気化学分析 / バイオ計測 / バイオMEMS / マイクロ・ナノ化学 / 微小電極アレイ / 細胞解析 / ナノ・マイクロ化学 / バイオイメージング

Outline of Final Research Achievements

This study presents the molecular electrochemical switching element based on molecular diffusion and electrochemical reaction. The element acts like a transistor. The elements were incorporated into a small chip device, and the device was characterized. The device was applied to evaluation of cell differentiation of embryonic stem cells and respiration activity of cancer cells. In addition to them, the novel electrochemical imaging system was developed using CMOS-based electrode arrays. By using the device, multiple analytes were electrochemically imaged at the same time.

Research Products

• [Int'l Joint Research] Izmir Katip Celebi University(トルコ)
• [Int'l Joint Research] Florida International University(米国)
• [Journal Article] Micropatterning of Nafion Membranes on an Electrode Array Using Photolithographic and Lift-off Techniques for Selective Electrochemical Detection and Signal Accumulation (2018)
  • Author(s): Terauchi Mayuko、Ino Kosuke、Kanno Yusuke、Imai Shunsuke、Shiku Hitoshi、Matsue Tomokazu
  • Journal Title: Chemistry Letters Volume: 47 Issue: 2 Pages: 204-206
  • DOI: 10.1246/cl.171031
  • NAID: 130006337743
  • ISSN: 0366-7022, 1348-0715
  • Peer Reviewed
• [Journal Article] Electrochemicolor imaging of endogenous alkaline phosphatase and respiratory activities of mesenchymal stem cell aggregates in early-stage osteodifferentiation (2018)
  • Author(s): Ino Kosuke、Onodera Takehiro、Kanno Yusuke、Suda Atsushi、Kunikata Ryota、Matsue Tomokazu、Shiku Hitoshi
  • Journal Title: Electrochimica Acta Volume: 268 Pages: 554-561
  • DOI: 10.1016/j.electacta.2018.02.094
  • Peer Reviewed
• [Journal Article] Binary-number-based digital electrochemical detection using a single working electrode with multiple sensors (2017)
  • Author(s): K. Ino, Y. Kanno, Y. Yamada, H. Shiku, T. Matsue
  • Journal Title: Electrochemistry Communications Volume: 77 Pages: 76-80
  • DOI: 10.1016/j.elecom.2017.02.016
  • Peer Reviewed
• [Journal Article] Electrochemical motion tracking of microorganisms using a large-scale integration (LSI)-based amperometric device (2017)
  • Author(s): Kosuke Ino, Yusuke Kanno, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue
  • Journal Title: Angewandte Chemie International Edition Volume: 56 Issue: 24 Pages: 6818-6822
  • DOI: 10.1002/anie.201701541
  • Peer Reviewed
• [Journal Article] Bioelectrochemical applications of microelectrode arrays in cell analysis and engineering (2017)
  • Author(s): Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue
  • Journal Title: Current Opinion in Electrochemistry Volume: 5 Issue: 1 Pages: 146-151
  • DOI: 10.1016/j.coelec.2017.08.004
  • Peer Reviewed
• [Journal Article] Development of oxygen consumption analysis with an on-chip electrochemical device and simulation (2017)
  • Author(s): Hiramoto Kaoru、Yasumi Masahiro、Ushio Hiroshi、Shunori Atsushi、Ino Kosuke、Shiku Hitoshi、Matsue Tomokazu
  • Journal Title: Analytical Chemistry Volume: 89 Issue: 19 Pages: 10303-10310
  • DOI: 10.1021/acs.analchem.7b02074
  • Peer Reviewed
• [Journal Article] Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells (2017)
  • Author(s): Kosuke Ino, Mustafa Sen, Hitoshi Shiku, Tomokazu Matsue
  • Journal Title: Analyst Volume: 142 Issue: 23 Pages: 4343-4354
  • DOI: 10.1039/c7an01442b
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Electrochemicolor imaging using an LSI-Based device for multiplexed cell assays (2017)
  • Author(s): Yusuke Kanno, Kosuke Ino, Hiroya Abe, Chika Sakamoto, Takehiro Onodera, Kumi Y Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue
  • Journal Title: Analytical Chemistry Volume: 89 Issue: 23 Pages: 12778-12786
  • DOI: 10.1021/acs.analchem.7b03042
  • Peer Reviewed / Open Access
• [Journal Article] Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device (2016)
  • Author(s): Abe H, Kanno Y, Ino K, Inoue KY, Suda A, Kunikata R, Matsudaira M, Shiku H, Matsue T
  • Journal Title: Electrochemistry Volume: 84 Issue: 5 Pages: 364-367
  • DOI: 10.5796/electrochemistry.84.364
  • NAID: 130005150427
  • ISSN: 1344-3542, 2186-2451
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Potentiometric bioimaging with a large-scale integration (LSI)-based electrochemical device for detection of enzyme activity (2016)
  • Author(s): Kanno Y, Ino K, Sakamoto C, Inoue KY, Matsudaira M, Suda A, Kunikata R, Ishikawa T, Abe H, Shiku H, Matsue T
  • Journal Title: Biosens. Bioelectron. Volume: 77 Pages: 709-714
  • DOI: 10.1016/j.bios.2015.10.021
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular electrochemical switching element based on diffusive molecular competition for multipoint electrochemical detection of respiration activity of cell aggregates (2016)
  • Author(s): Ino K, Yamada Y, Kanno Y, Imai S, Shiku H, Matsue T
  • Journal Title: Sensors and Actuators B: Chemical Volume: 234 Pages: 201-208
  • DOI: 10.1016/j.snb.2016.04.160
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A local redox cycling-based electrochemical chip device with nanocavities and microwells for multi-electrochemical detection of alkaline phosphatase activity in embryoid bodies from embryonic stem cells (2015)
  • Author(s): Kanno Y, Ino K, Shiku H, Matsue T
  • Journal Title: Lab Chip Volume: 15 Issue: 23 Pages: 4404-4414
  • DOI: 10.1039/c5lc01016k
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Electrochemical imaging of dopamine release from three-dimensional-cultured PC12 cells using LSI-based amperometric sensors (2015)
  • Author(s): Abe H, Ino K, Li CZ, Kanno Y, Inoue KY, Suda A, Kunikata R, Matsudaira M, Takahashi Y, Shiku H, Matsue T
  • Journal Title: Anal. Chem. Volume: 87 Issue: 12 Pages: 6364-6370
  • DOI: 10.1021/acs.analchem.5b01307
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications (2015)
  • Author(s): Ino K
  • Journal Title: Electrochemistry Volume: 83 Issue: 9 Pages: 688-694
  • DOI: 10.5796/electrochemistry.83.688
  • NAID: 130005095965
  • ISSN: 1344-3542, 2186-2451
  • Acknowledgement Compliant
• [Journal Article] Local Redox-cycling-based Electrochemical System for Bioimaging (2015)
  • Author(s): 伊野浩介、珠玖仁、末永智一
  • Journal Title: BUNSEKI KAGAKU Volume: 64 Issue: 9 Pages: 669-678
  • DOI: 10.2116/bunsekikagaku.64.669
  • NAID: 130005102583
  • ISSN: 0525-1931
• [Presentation] Integrated electrode array device for cell analysis (2018)
  • Author(s): Kosuke Ino
  • Organizer: 日本化学会第98春季年会（アジア国際シンポジウム）
  • Int'l Joint Research / Invited
• [Presentation] マイクロ・ナノ電気化学デバイスによる細胞活性イメージング (2017)
  • Author(s): 伊野浩介
  • Organizer: 平成29年度 第1回研究会（マルチモーダルバイオイメージセンサ研究会）
  • Invited
• [Presentation] Integrated electrochemical devices based on micro/nano-chemistry for bioassays (2017)
  • Author(s): Kosuke Ino
  • Organizer: 68th Annual Meeting of the International Society of Electrochemistry
  • Int'l Joint Research
• [Presentation] Hydrogel fabrication based on electrodeposition using electrochemical devices (2017)
  • Author(s): Kosuke Ino, Mayuko Terauchi, Atsushi Suda, Ryota Kunikata, Tomokazu Matsue, Hitoshi Shiku
  • Organizer: 68th Annual Meeting of the International Society of Electrochemistry
  • Int'l Joint Research
• [Presentation] 電気化学カラーイメージングによる細胞呼吸活性、分泌活性、酵素活性評価 (2017)
  • Author(s): 伊野浩介、菅野佑介、小野寺岳大、須田篤史、國方亮太、末永智一、珠玖仁
  • Organizer: 第11回バイオ関連化学シンポジウム
• [Presentation] Electrode array devices for evaluation of three-dimensional cultured cells (2017)
  • Author(s): Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku
  • Organizer: The International Workshp: New Electroanalytical Techniques and Their Emerging Applications
  • Int'l Joint Research / Invited
• [Presentation] チップデバイスへの多数の電気化学センサの組み込みに関する戦略 (2017)
  • Author(s): 伊野浩介、末永智一、珠玖仁
  • Organizer: バイオ・マイクロシステム研究会
• [Presentation] LSI-based electrochemical device for evaluation of respiratory activity of three-dimensional cultured cells (2017)
  • Author(s): Kosuke Ino
  • Organizer: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
  • Int'l Joint Research
• [Presentation] 3次元培養細胞の評価に向けた電気化学デバイスの開発 (2017)
  • Author(s): 伊野浩介
  • Organizer: 第5回細胞凝集研究会
  • Invited
• [Presentation] 集積化した電気化学センサデバイスによるバイオ計測 (2017)
  • Author(s): 伊野浩介
  • Organizer: H29年度 第3回 信州MEMS研究会
  • Invited
• [Presentation] Multi-electrode array device for electrochemical imaging of cell activity (2017)
  • Author(s): Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue
  • Organizer: Pittcon 2017
  • Int'l Joint Research
• [Presentation] バイオLSIを用いたバイオ計測 (2017)
  • Author(s): 伊野浩介
  • Organizer: マイクロシステム融合研究開発センターシンポジウム 文部科学省先端融合領域イノベーション創出拠点形成プログラム マイクロシステム融合研究開発拠点成果最終報告会
• [Presentation] Electrochemicolor imaging for cell analysis (2017)
  • Author(s): Kosuke Ino, Yusuke Kanno, Hitoshi Shiku, Tomokazu Matsue
  • Organizer: 日本化学会第97春季年会(2017)
• [Presentation] Micro/nano electrochemical device for bioassays (2017)
  • Author(s): Kosuke Ino
  • Organizer: 日本化学会第97春季年会(2017)
• [Presentation] マイクロ・ナノ化学に基づく電気化学イメージングデバイスの開発と細胞解析への応用 (2016)
  • Author(s): 伊野浩介
  • Organizer: 日本化学会第96回春季大会（2016）
  • Place of Presentation: 同志社大学（京都）
  • Year and Date: 2016-03-24
  • Invited
• [Presentation] 電気化学バイオイメージングのカラー化に向けたLSIデバイスの開発 (2016)
  • Author(s): 伊野浩介、菅野佑介、須田篤史、國方亮太、松平昌昭 、珠玖仁 、末永智一
  • Organizer: 日本化学会第96回春季大会（2016）
  • Place of Presentation: 同志社大学（京都）
  • Year and Date: 2016-03-24
• [Presentation] マイクロ・ナノ化学に基づく電気化学バイオイメージングデバイス (2016)
  • Author(s): 伊野浩介、珠玖仁、末永智一
  • Organizer: バイオ・マイクロシステム研究会
• [Presentation] Electrochemical imaging for respiration activity and dopamine release of spheroids using a color amperometric device (2016)
  • Author(s): Kosuke Ino, Yusuke Kanno, Hitoshi Shiku, Tomokazu Matsue
  • Organizer: 8th International Symposium on Microchemistry and Microsystems (ISMM2016)
  • Int'l Joint Research
• [Presentation] マイクロ・ナノ電気化学に基づくバイオイメージングデバイス (2016)
  • Author(s): 伊野浩介
  • Organizer: 2016年度第1回界面ナノ科学研究会
• [Presentation] Colorization of electrochemical imaging for cell analysis using a large-scale integration (LSI) device (2016)
  • Author(s): Kosuke Ino, Yusuke Kanno, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue
  • Organizer: 67th Annual Meeting of the International Society of Electrochemistry
  • Int'l Joint Research
• [Presentation] Molecular electrochemical switching with nano- and microstructures (2016)
  • Author(s): Kosuke Ino, Yusuke Kanno, Hitoshi Shiku, Tomokazu Matsue
  • Organizer: 67th Annual Meeting of the International Society of Electrochemistry
  • Int'l Joint Research
• [Presentation] Integrated electrochemical imaging device for cell analysis (2016)
  • Author(s): Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue
  • Organizer: 第3回アジア分析科学シンポジウム
• [Presentation] アルギン酸カルシウム犠牲層を用いたゼラチンハイドロゲルの3次元構築 (2016)
  • Author(s): 伊野浩介、横川裕紀、珠玖仁、末永智一
  • Organizer: 第68回日本生物工学会大会
• [Presentation] Digital electrochemical array detection on a single (2016)
  • Author(s): Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue
  • Organizer: The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS 2016)
  • Int'l Joint Research
• [Presentation] バイオイメージングに向けた電気化学デバイスの開発 (2016)
  • Author(s): 伊野浩介
  • Organizer: 第３回学産交流ポスターセッション「エレクトロニクスの未来を支える新規素材・技術・プロセス」
• [Presentation] Molecular diffusion-based switching system using microelectrode array (2015)
  • Author(s): Ino K, Yamada Y, Shiku H, Matsue T
  • Organizer: Pacifichem 2015
  • Place of Presentation: Hawaii (USA)
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Electrochemical imaging using molecular consumption-based switching system for evaluation of respiration activity of cell aggregates (2015)
  • Author(s): Ino K, Yamada Y, Shiku H, Matsue T
  • Organizer: The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2015)
  • Place of Presentation: Gyeongju (Korea)
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] 分子電気化学スイッチングデバイスを用いた3次元培養組織の呼吸活性イメージング (2015)
  • Author(s): 伊野浩介、山田祐大、菅野佑介、珠玖仁、末永智一
  • Organizer: 第9回バイオ関連化学シンポジウム ―第30回生体機能関連化学シンポジウム，第18回バイオテクノロジー部会シンポジウム―
  • Place of Presentation: 熊本大学（熊本）
  • Year and Date: 2015-09-10
• [Presentation] Large-scale integration (LSI)-based electrochemical chip device (2015)
  • Author(s): Ino K, Kanno Y, Abe H, Inoue KY, Matsudaira M, Suda A, Kunikata R, Shiku H, Matsue T
  • Organizer: RSC Tokyo International Conference 2015
  • Place of Presentation: 幕張メッセ（千葉）
  • Year and Date: 2015-09-03
  • Int'l Joint Research
• [Presentation] マイクロ・ナノ化学を利用した電気化学バイオ分析 (2015)
  • Author(s): 伊野浩介
  • Organizer: 平成27年度日本分析化学会東北支部若手交流会
  • Place of Presentation: パルセ飯坂（福島）
  • Year and Date: 2015-07-17
  • Invited
• [Presentation] Electrochemical bioimaging using a diffusion control-based electrode array device (2015)
  • Author(s): Ino K, Yamada Y, Kanno Y, Shiku H, Matsue T
  • Organizer: 7th International Symposium on Microchemistry and Microsystems (ISMM2015)
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2015-06-08
  • Int'l Joint Research
• [Presentation] 分子電気化学スイッチング素子を用いた細胞呼吸活性の電気化学イメージング (2015)
  • Author(s): 伊野浩介、山田祐大、菅野佑介、珠玖仁、末永 智一
  • Organizer: 第75回分析化学討論会
  • Place of Presentation: 山梨大学（山梨）
  • Year and Date: 2015-05-23
• [Presentation] マイクロ・ナノ化学と電気化学の融合による新規バイオ分析法の開発 (2015)
  • Author(s): 伊野浩介
  • Organizer: 2015年度バイオインダストリー協会賞 発酵と代謝研究奨励賞、化学・生物素材研究開発奨励賞 合同発表会（招待講演）
  • Place of Presentation: パシフィコ横浜（横浜）
  • Invited
• [Patent(Industrial Property Rights)] 電気化学イメージング方法、電気化学測定装置及びトランスデューサ (2016)
  • Inventor(s): 伊野他
  • Industrial Property Rights Holder: 伊野他
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-101043
  • Filing Date: 2016