Inclusion of biopolymers within porous peptide crystals and their applications

• Project/Area Number: 15H05481
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Functional solid state chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Sawada Tomohisa 東京大学, 大学院工学系研究科(工学部), 准教授 (70625467)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000); Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2015: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
• Keywords: 自己組織化 / ペプチド / 配位結合 / フォールディング / ナノ構造 / 結晶材料 / ナノ材料 / 分子認識 / 超分子化学

Outline of Final Research Achievements

A porous peptide crystal, spontaneously self-assembled by simple mixing of a short peptide and a metal ion, has a precise nano-cavity via the facile synthesis. In this work, we succeeded in syntheses of a series of such porous crystals from 1.5 to 2.8 nanometer-sized cavities via chemical modifications of peptides, and revealed their molecular inclusion properties based on their cavity sizes. Furthermore, we also succeeded in creation of uniquely entangled molecules and a precise mimic of natural β-barrels via peptide sequence designs.

Academic Significance and Societal Importance of the Research Achievements

自然界のタンパク質の精密かつ機能的な分子構造は、長いペプチド鎖の非常に複雑な自己組織化過程を経て構築され、これを人工的に模倣することは合成学的に未だ不可能である。本研究では、短いペプチド鎖と金属イオンを混ぜ合わせる独自の自己組織化方法を考案し、簡便な合成法ながら様々な新奇ナノ構造の構築に成功した。これらはタンパク質の精密な模倣を可能にするとともに、前例のないユニークなトポロジー構造も含まれ、ナノ構造構築における革新的な合成手法としての学術的意義を示すものである。

Research Products

• [Int'l Joint Research] Universite de Bordeaux(フランス)
• [Journal Article] Metal-peptide rings form highly entangled topologically inequivalent frameworks with the same ring- and crossing-numbers (2019)
  • Author(s): T. Sawada, A. Saito, K. Tamiya, K. Shimokawa, Y. Hisada, M. Fujita
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 921-921
  • DOI: 10.1038/s41467-019-08879-7
  • Peer Reviewed / Open Access
• [Journal Article] Synthetic β-Barrel by Metal-Induced Folding and Assembly (2018)
  • Author(s): Yamagami Motoya、Sawada Tomohisa、Fujita Makoto
  • Journal Title: Journal of the American Chemical Society Volume: 140 Issue: 28 Pages: 8644-8647
  • DOI: 10.1021/jacs.8b04284
  • Peer Reviewed
• [Journal Article] Self-Assembly of a Peptide [2]Catenane through Ω-Loop Folding (2017)
  • Author(s): Tomohisa Sawada, Yuuki Inomata, Motoya Yamagami, Makoto Fujita
  • Journal Title: Chem. Lett. Volume: 46 Issue: 8 Pages: 1119-1121
  • DOI: 10.1246/cl.170438
  • NAID: 130005906942
  • Peer Reviewed / Open Access
• [Journal Article] Porous Peptide Complexes by a Folding-and-Assembly Strategy (2017)
  • Author(s): Tomohisa Sawada, Motoya Yamagami, Shuji Akinaga, Tatsuki Miyaji, Makoto Fujita
  • Journal Title: Chem. Asian J. Volume: 12 Issue: 14 Pages: 1715-1718
  • DOI: 10.1002/asia.201700458
  • Peer Reviewed / Open Access
• [Journal Article] Peptide [4]Catenane by Folding and Assembly (2016)
  • Author(s): T. Sawada, M. Yamagami, K. Ohara, K. Yamaguchi, M. Fujita
  • Journal Title: Angew. Chem. Int. Ed. Volume: 55 Issue: 14 Pages: 4519-4522
  • DOI: 10.1002/anie.201600480
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Concerted folding-and-assembly of short peptides via coordination (2018)
  • Author(s): Tomohisa Sawada
  • Organizer: The Wolf Prize Symposium
  • Int'l Joint Research / Invited
• [Presentation] Peptide-entangled polyhedra by folding and assembly (2018)
  • Author(s): Tomohisa Sawada, Makoto Fujita
  • Organizer: 43rd International Conference on Coordination Chemistry
  • Int'l Joint Research / Invited
• [Presentation] Higher-order peptide nanostructures via metal-induced folding and assembly (2018)
  • Author(s): Tomohisa Sawada
  • Organizer: Bordeaux 2018 Symposium on Foldamers
  • Int'l Joint Research
• [Presentation] Unique peptide nanostructures via metal-directed folding and assembly (2018)
  • Author(s): Tomohisa Sawada, Makoto Fujita
  • Organizer: International Congress on Pure and Applied Chemistry 2018
  • Int'l Joint Research / Invited
• [Presentation] Metal-peptide nanostructures with unique entanglements (2018)
  • Author(s): Tomohisa Sawada, Makoto Fujita
  • Organizer: 6th International Conference on Metal-Organic Frameworks and Open Framework Compounds
  • Int'l Joint Research / Invited
• [Presentation] Unique entangled nanostructures via metal-directed folding-and-assembly (2018)
  • Author(s): Tomohisa Sawada, Motoya Yamagami, Shuji Akinaga, Ami Saito, Yuuki Inomata, Makoto Fujita
  • Organizer: The 6th International Symposium “Dynamical ordering of biomolecular systems for creation of integrated functions”
  • Int'l Joint Research
• [Presentation] Creation of Higher-order Peptidic Nanostructures by Concerted Folding and Assembly (2018)
  • Author(s): 澤田 知久
  • Organizer: 日本化学会第98春季年会
  • Invited
• [Presentation] A Synthetic Protein-like Tetragonal Scaffold (2017)
  • Author(s): Tomohisa Sawada, Motoya Yamagami, Shuji Akinaga, Tatsuki Miyaji, Makoto Fujita
  • Organizer: The 5th International Symposium "Dynamic ordering of biomolecular systems for creation of integrated functions"
  • Place of Presentation: 東京大学駒場キャンパス（東京都目黒区）
  • Year and Date: 2017-01-21
  • Int'l Joint Research
• [Presentation] Metal-peptide nanostructures via concerted folding-and-assembly (2017)
  • Author(s): Tomohisa Sawada, Makoto Fujita
  • Organizer: Gordon Research Conference, “Self-Assembly & Supramolecular Chemistry”
  • Int'l Joint Research
• [Presentation] Concerted folding-and-assembly of short peptides via coordination (2017)
  • Author(s): Tomohisa Sawada, Makoto Fujita
  • Organizer: The 12th International Symposium on Macrocyclic and Supramolecular Chemistry (in conjugation with ISACS)
  • Int'l Joint Research
• [Presentation] Metal-peptide nanostructures with unique entanglements (2017)
  • Author(s): Tomohisa Sawada
  • Organizer: Symposium on Frontier of Biofunctional Chemistry (ISBC 2017 Pre-symposium Meeting)
  • Int'l Joint Research / Invited
• [Presentation] 配位結合とペプチド配座を利用した新奇ナノ構造の創出 (2017)
  • Author(s): 澤田 知久
  • Organizer: 最先端光計測とライフサイエンスの近未来 -Bio. Phys. Chem. 三重点の探索-（2017年光化学討論会 共催シンポジウム）
  • Invited
• [Presentation] Porous peptide complexes based on polyproline II helices (2017)
  • Author(s): 澤田 知久、山上 樹也、秋永 修志、宮路 達貴、藤田 誠
  • Organizer: 第67回錯体化学討論会
• [Presentation] Metal-directed short peptide folding and assembly for unique entangled nanostructures (2015)
  • Author(s): Tomohisa Sawada, Asami Matsumoto, Motoya Yamagami, Makoto Fujita
  • Organizer: Pacifichem 2015 The International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: ホノルル（米国）
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Artificial Peptide-Based Nanostructures via Coordination-driven Folding and Assembly (2015)
  • Author(s): Tomohisa Sawada, Motoya Yamagami, Yusuke Otsubo, Asami Matsumoto, Makoto Fujita
  • Organizer: The 4th International Symposium “Dynamical ordering of biomolecular systems for creation of integrated functions”
  • Place of Presentation: 九州大学西新プラザ（福岡県福岡市）
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Metal-Directed Short Peptide Folding and Assembly (2015)
  • Author(s): Tomohisa Sawada, Asami Matsumoto, Motoya Yamagami, Makoto Fujita
  • Organizer: Supramolecular Dynamics at the Interface of Chemistry and Biology
  • Place of Presentation: 分子科学研究所山手キャンパス（愛知県岡崎市）
  • Year and Date: 2015-07-11
  • Int'l Joint Research
• [Presentation] Entangled peptide nanostructures via metal-directed folding and assembly (2015)
  • Author(s): Tomohisa Sawada, Asami Matsumoto, Motoya Yamagami, Makoto Fujita
  • Organizer: 10th ISMSC-2015
  • Place of Presentation: ストラスブール（フランス）
  • Year and Date: 2015-06-28
  • Int'l Joint Research