| Budget Amount *help |
¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2015: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
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| Outline of Final Research Achievements |
We developed a new bioluminescence tool for monitoring protein dynamics in living cells including cell to cell communication, with newly constructed a microscope observation system. For tracing the temporal changing of cell activity rapidly, we choose the novel luciferase species that has sufficiently high luminescence intensity. We have already established an optical technique for monitoring protein-protein interactions in living cells based on complementation of split fragments of luciferase. In this case, the luciferase was also used as split fragments to monitor the protein that plays a key role in target phenomenon. We also developed a bioluminescence observation system based inverted fluorescence microscope, the set-up in which was with high sensitive and darkness condition. Finally, the luminescent probe in combination with the observation system monitored GPCR activities and the temporal correlation between cell and cell.
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