Amino acid substitution without genetic modification
Project/Area Number |
15H05491
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Bio-related chemistry
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Research Institution | Nagoya University |
Principal Investigator |
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Research Collaborator |
YAMASHITA Shun
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2017: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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Keywords | 革新的遺伝子治療 / 核酸医薬 / アンチセンス / ナンセンス変異 / リードスルー / 核酸 / 糖 / リボソーム結合分子 / 生体分子 / 遺伝情報変換 |
Outline of Final Research Achievements |
A nonsense mutation is a genetic mutation that converts a codon coding an amino acid to the stop codon called premature termination codon (PTC). This mutation causes a variety of diseases including Duchenne muscular dystrophy and cystic fibrosis. One of the potential methods for treating such diseases is readthrough therapy that is accomplished by the incorporation of an amino acid to PTC during translation and hence produces the full-length proteins. So far, aminoglycosides and other synthetic compounds that reduce the translation fidelity have been utilized to readthrough therapy. However, their toxicity due to the non-specific induction of inaccurate translation hampered their clinical use. In this study, we have developed a novel readthrough strategy that enables the target PTC-specific readthrough.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Probing strigolactone receptors in Striga hermonthica with fluorescence.2015
Author(s)
Tsuchiya, Y., Yoshimura, M., Sato, Y., Kuwata, K., Toh, S., Holbrook-Smith, D., Zhang, H., McCourt, P., Itami, K., Kinoshita, T. and Hagihara, S.
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Journal Title
Science
Volume: 349
Issue: 6250
Pages: 864-869
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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