Artificial photosynthesis system constructed on DNA nanoreactor

• Project/Area Number: 15H05492
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Bio-related chemistry
• Research Institution: Kyoto University
• Principal Investigator: NAKATA EIJI 京都大学, エネルギー理工学研究所, 准教授 (70467827)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: DNAナノ構造体 / DNAナノリアクター / 多段階物質変換反応 / 人工光合成システム / モジュール型アダプター / 共有結合型アダプター / ナノリアクター

Outline of Final Research Achievements

Recent development in the field of structural DNA nanotechnology has enabled the controlled self-assembly of biomacromolecules and nanomaterials. The precise and programmable base-pairing of DNA is utilized to provide fully addressable nanoarchitectures of various shapes and geometries as ideal scaffolds to position the molecules of interest with nanometer-level precision.
DNA binding-based adaptors have the potential to be an ideal protein-DNA conjugation method　to locate several kinds of proteins of interests at desired positions on the DNA scaffold with orthogonality and fast reaction kinetics to realize quantitative loading yields. By using a series of DNA binding adaptors, an enzyme cascade reaction system was constructed on the DNA scaffold.

Research Products

• [Journal Article] DNA Origami Scaffolds as Templates for Functional Tetrameric Kir3 K+ Channels (2018)
  • Author(s): Kurokawa Tatsuki、Kiyonaka Shigeki、Nakata Eiji、Endo Masayuki、Koyama Shohei、Mori Emiko、Tran Nam Ha、Dinh Huyen、Suzuki Yuki、Hidaka Kumi、Kawata Masaaki、Sato Chikara、Sugiyama Hiroshi、Morii Takashi、Mori Yasuo
  • Journal Title: Angew. Chem. Int. Ed. Engl. Volume: 57 Issue: 10 Pages: 2586-2591
  • DOI: 10.1002/anie.201709982
  • Peer Reviewed / Open Access
• [Journal Article] 効率的な多段階酵素反応を実現する分子コンビナート (2018)
  • Author(s): 中田栄司，森井孝
  • Journal Title: バイオサイエンスとインダストリー Volume: @
  • NAID: 40021446902
• [Journal Article] 酵素を“1分子ずつ”狙った場所に配置する-多段階反応を効率的に進行させる分子コンビナートをつくる (2017)
  • Author(s): 中田栄司，森井孝
  • Journal Title: Academist Journal Volume: @
• [Journal Article] ナノレベル間隔で異なる酵素を並べる！─複雑な多段階反応を可能にする分子コンビナートへの挑戦─ (2017)
  • Author(s): 中田栄司，森井孝
  • Journal Title: 化学 Volume: 11月号
  • NAID: 40021363518
• [Journal Article] Design of Modular Protein-Tags for the Orthogonal Covalent Bond Formation at Specific DNA Sequences (2017)
  • Author(s): T.M. Nguyen, E. Nakata, M. Saimura, H. Dinh, T. Morii
  • Journal Title: Journal of the American Chemical Society Volume: 139 Issue: 25 Pages: 8487-8496
  • DOI: 10.1021/jacs.7b01640
  • Peer Reviewed
• [Journal Article] Nucleic acids-templated enzyme cascades (2017)
  • Author(s): A. Rajendran, E. Nakata, S. Nakano, T. Morii
  • Journal Title: ChemBioChem Volume: 18 Issue: 8 Pages: 696-716
  • DOI: 10.1002/cbic.201600703
  • Peer Reviewed
• [Journal Article] 若手研究者からのメッセージ (2017)
  • Author(s): 中田 栄司
  • Journal Title: バイオテクノロジー部会 NEWS LETTER Volume: 20 Pages: 7-7
• [Journal Article] DNAナノ構造体に酵素を配置した分子スイッチボード (2016)
  • Author(s): 中田 栄司
  • Journal Title: 生体機能関連化学部会 NEWS LETTER Volume: 3 Pages: 3-3
• [Journal Article] Spatially Organized Enzymes Drive Cofactor-Coupled Cascade Reactions. (2016)
  • Author(s): T. A. Ngo, E. Nakata, M. Saimura, T. Morii
  • Journal Title: J. Am. Chem. Soc. Volume: 138 Issue: 9 Pages: 3012-3021
  • DOI: 10.1021/jacs.5b10198
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on DNA scaffold (2017)
  • Author(s): E. Nakata, T. Morii
  • Organizer: ISPAC_2017
  • Int'l Joint Research / Invited
• [Presentation] 高効率な多段階酵素反応を実現するDNAナノリアクターの創製酵素を “1分子ずつ” 狙った場所に配置する (2017)
  • Author(s): 中田栄司，森井孝
  • Organizer: 生体機能関連化学部会若手の会 第29回サマースクール
  • Invited
• [Presentation] 分子コンビナートよる非天然化合物合成システムの創製 (2017)
  • Author(s): 中田栄司，森井孝
  • Organizer: 新学術領域「生合成リデザイン」第2回公開シンポジウム
  • Invited
• [Presentation] 直交性を有するモジュール型アダプターによる複数の酵素を配置した分子スイッチボード (2017)
  • Author(s): 中田栄司，Nguyen Minh Thang，才村正幸，Dinh Huyen，森井孝
  • Organizer: 第11回バイオ関連化学シンポジウム
• [Presentation] Activity of the assembled enzyme on DNA scaffold (2017)
  • Author(s): H. Dinh, E. Nakata, T. Morii
  • Organizer: 第11回バイオ関連化学シンポジウム
• [Presentation] Assembly of DNA Rotaxane and Catenane Inside a DNA Origami Frame (2017)
  • Author(s): A. Rajendran, S.-J. Park, E. Nakata, Y. Kwon, T. Morii
  • Organizer: 第11回バイオ関連化学シンポジウム
• [Presentation] 酵素を“1分子ずつ”狙った場所に配置するー試験管内での高効率な多段階反応システムへの挑戦ー (2017)
  • Author(s): E. Nakata, T. Morii
  • Organizer: 2nd NNBS
  • Invited
• [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on DNA scaffold (2017)
  • Author(s): E. Nakata, T. Morii
  • Organizer: Nano-S&T-2017
  • Int'l Joint Research / Invited
• [Presentation] 生物から学んだ分子コンビナートへの挑戦 (2017)
  • Author(s): 中田栄司
  • Organizer: 京都大学宇治キャンパス公開 特別講演会
• [Presentation] DNAナノ構造体上に酵素を配置した分子コンビナート (2017)
  • Author(s): 中田栄司，森井孝
  • Organizer: 「統合物質創製化学研究推進機構」国内シンポジウム
  • Invited
• [Presentation] Construction of Topologically Interlocked DNAs Inside a DNA Scaffold (2017)
  • Author(s): A. Rajendran, S.-J. Park, E. Nakata, Y. Kwon, T. Morii
  • Organizer: 第44回国際核酸化学シンポジウム/日本核酸化学会第1会年会The 44th International Symposium on Nucleic Acids Chemistry /The 1st Annual Meeting of Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Orthogonal Modular Adaptors to Locate Three Enzymes for Cascade Reaction on DNA Scaffold (2017)
  • Author(s): T. M. Nguyen, E. Nakata, M. Saimura, H. Dinh, T. Morii
  • Organizer: 第44回国際核酸化学シンポジウム/日本核酸化学会第1会年会The 44th International Symposium on Nucleic Acids Chemistry /The 1st Annual Meeting of Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Reaction of enzymes in the ordered assembly states on DNA nanostructures (2017)
  • Author(s): H. Dinh, E. Nakata, T. Morii
  • Organizer: 第44回国際核酸化学シンポジウム/日本核酸化学会第1会年会The 44th International Symposium on Nucleic Acids Chemistry /The 1st Annual Meeting of Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] DNAナノ構造体上に複数種類の酵素を配置した分子コンビナート (2017)
  • Author(s): 中田栄司, 森井孝
  • Organizer: 第6回植物二次代謝フロンティア研究会
  • Invited
• [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on DNA Scaffold (2017)
  • Author(s): E. Nakata, T. Morii
  • Organizer: ISBC2017 The Second International Symposium on Biofunctional Chemistry
  • Int'l Joint Research / Invited
• [Presentation] Assembly of multiple enzymes on a DNA scaffold (2017)
  • Author(s): T.M. Nguyen, E. Nakata, M. Saimura, H. Dinh, T. Morii
  • Organizer: ISBC2017 The Second International Symposium on Biofunctional Chemistry
  • Int'l Joint Research
• [Presentation] DNA rotaxane and catenane inside a DNA nanostructure (2017)
  • Author(s): A. Rajendran, S-J. Park, E. Nakata, Y. Kwon, T. Morii
  • Organizer: ISBC2017 The Second International Symposium on Biofunctional Chemistry
  • Int'l Joint Research
• [Presentation] 酵素を“1分子ずつ”狙った場所に配置する ー生物から学んだ分子コンビナートへの挑戦― (2017)
  • Author(s): 中田栄司，森井孝
  • Organizer: 北九州工業高等専門学校 講演会
  • Invited
• [Presentation] 酵素を“1分子ずつ”狙った場所に配置する ー生物から学んだ分子コンビナートへの挑戦― (2017)
  • Author(s): 中田栄司，森井孝
  • Organizer: 第11回バイオ＆マテリアル講演会
  • Invited
• [Presentation] Efficiency of The Enzyme Cascade Reaction on a DNA Scaffold (2017)
  • Author(s): LIN, Peng; NGUYEN, Thang; DINH, Huyen; NAKATA, Eiji; MORII, Takashi
  • Organizer: 日本化学会第98春季年会2018
• [Presentation] Reaction of Carbonic Anhydrase assembled on DNA scaffold (2017)
  • Author(s): DINH, Huyen; NAKATA, Eiji; KINOSHITA, Masahiro; MORII, Takashi
  • Organizer: 日本化学会第98春季年会2018
• [Presentation] Supramolecular Assemblies of Mechanically Interlocked Components Inside a DNA Origami Frame (2017)
  • Author(s): RAJENDRAN, Arivazhagan; PARK, Seo-jeong; NAKATA, Eiji; KWON, Youngjoo; MORII, Takashi
  • Organizer: 日本化学会第98春季年会2018
• [Presentation] Sequence Specific Modular Adaptors for Orthogonal Location of Multiple Enzymes on a DNA scaffold (2017)
  • Author(s): NGUYEN, Thang; NAKATA, Eiji; SAIMURA, Masayuki; MORII, Takashi
  • Organizer: 日本化学会第98春季年会2018
• [Presentation] DNAナノ構造体を鋳型にしたナノリポソーム (2017)
  • Author(s): 小西 宏明・DINH Huyen・中田 栄司・仲野 瞬・森井 孝
  • Organizer: 日本化学会第98春季年会2018
• [Presentation] 多数のタンパク質を配置したDNAナノ構造体の構築 (2017)
  • Author(s): 中田 栄司・DINH Huyen・才村 正幸・森井 孝
  • Organizer: 日本化学会第98春季年会2018
• [Presentation] 酵素を“1分子ずつ”狙った場所に配置したDNAナノリアクターの創製 (2017)
  • Author(s): 中田栄司，森井孝
  • Organizer: 日本物理学会第73回年次大会（2018年）
  • Invited
• [Presentation] 非天然化合物合成を目指した分子コンビナートの創製 (2017)
  • Author(s): 中田栄司，森井孝
  • Organizer: 生物機能化学シンポジウム
  • Invited
• [Presentation] Factor governing the efficiency of cascade reactions by spacially organized enzymes (2016)
  • Author(s): Tien Anh Ngo, Eiji Nakata, Masayuki Saimura, Takashi Morii
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-24
• [Presentation] Development of orthogonal modular adaptars for assembling multiple proteins on DNA nanostructure (2016)
  • Author(s): Thang Minh Nguyen, Eiji Nakata, Masayuki Saimura, Takashi Morii
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-24
• [Presentation] Construction of an enzyme assembly on DNA scaffold via a modular adaptor (2016)
  • Author(s): Huyen Dinh, Eiji Nakata, Tien Anh Ngo, Masayuki Saimura, Takashi Morii
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-24
• [Presentation] Expansion of modular adaptors for covalently locating (2016)
  • Author(s): Eiji Nakata, Thang Minh Nguyen, Masayuki Saimura, Takashi Morii
  • Organizer: ICIRS16
  • Place of Presentation: 京都
  • Year and Date: 2016-03-07
  • Int'l Joint Research
• [Presentation] 高効率な多段階酵素反応を実現するDNAナノリアクターの創製 (2016)
  • Author(s): 中田栄司
  • Organizer: 新化学技術研究奨励賞受賞講演会
  • Invited
• [Presentation] DNA ナノ構造体に酵素を配置した分子スイッチボードの開発 (2016)
  • Author(s): 中田栄司，森井孝
  • Organizer: 第14回 ホスト・ゲスト化学シンポジウム
• [Presentation] DNAナノ構造体に酵素を配置した分子スイッチボード (2016)
  • Author(s): 中田栄司，Ngo Anh Tien，Dinh Huyen，Nguyen Minh Thang，才村正幸，森井孝
  • Organizer: 第10回バイオ関連化学シンポジウム
• [Presentation] Evaluation of orthogonal modular adaptors for assembling proteins on DNA scaffold (2016)
  • Author(s): T.M. Nguyen, E. Nakata, M. Saimura, T. Morii
  • Organizer: The 43rd International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Activity of the assembled enzyme on DNA scaff old (2016)
  • Author(s): H.T.T. Dinh, E. Nakata, T.A. Ngo, T. Morii
  • Organizer: The 43rd International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] The efficiency of cascade reactions by spatially organized enzymes on DNA scaffold (2016)
  • Author(s): E. Nakata, T.A. Ngo, M. Saimura, T. Morii
  • Organizer: The 43rd International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] DNAナノ構造体上で実現した高効率な多段階物質変換システム (2016)
  • Author(s): 中田栄司
  • Organizer: 2016年度化学・生物素材研究開発奨励賞受賞講演
  • Invited
• [Presentation] Topologically interlocked DNA nanostructures inside a DNA origami (2016)
  • Author(s): A. RAJENDRAN, S. PARK, E. NAKATA, Y. KWON, T. MORII
  • Organizer: 日本化学会第97 春季年会
• [Presentation] Activity of spatially organized enzyme on DNA scaffold (2016)
  • Author(s): H. DINH, E. NAKATA, T. MORII
  • Organizer: 日本化学会第97 春季年会
• [Presentation] Orthogonal modular adaptors for assembling multiple enzymes on DNA scaffold (2016)
  • Author(s): T. NGUYEN, E. NAKATA, M. SAIMURA, T. MORII
  • Organizer: 日本化学会第97 春季年会
• [Presentation] Enzymatic cascade reactions on the DNA scaffold (2016)
  • Author(s): E. NAKATA, H. DINH, T. NGUYEN, M. SAIMURA, T. MORII
  • Organizer: 日本化学会第97 春季年会
• [Presentation] DNA オリガミを利用したナノリポソーム (2016)
  • Author(s): 小西宏明・中田栄司・仲野瞬・森井孝
  • Organizer: 日本化学会第97 春季年会
• [Presentation] The rational design strategy for latent ratiometric fluorescent pH probes based on the self-assembled SNARF derivatives (2016)
  • Author(s): E. Nakata
  • Organizer: 10th International Symposium on Nanomedicine (ISNM2016)
  • Int'l Joint Research / Invited
• [Presentation] Development of various types of protein adaptors to locate a single molecule of functional protein on molecular switchboard (2015)
  • Author(s): Tien Anh Ngo, Eiji Nakata, Huyen Dinh, Thang Minh Nguyen,
  • Organizer: 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem2015)
  • Place of Presentation: Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Orthogonal assembly of RuBisCO and carbonic anhydrase on a DNA nanoscaffold (2015)
  • Author(s): Huyen Dinh, Eiji Nakata, Tien Anh Ngo, Takashi Morii
  • Organizer: 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem2015)
  • Place of Presentation: Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Development of modular adaptors to covalently locate multiple enzymes on DNA nanoscaffold (2015)
  • Author(s): Thang Minh Nguyen, Eiji Nakata, Huyen Dinh,Tien Anh Ngo, Masayuki Saimura, Takashi Morii
  • Organizer: 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem2015)
  • Place of Presentation: Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] DNAナノ構造体に機能性タンパク質を配置した分子スイッチボードの開発 (2015)
  • Author(s): 中田 栄司・森井 孝
  • Organizer: Workshop in 富山大学
  • Place of Presentation: 富山
  • Year and Date: 2015-11-18
  • Invited
• [Presentation] Orthogonal assembly of RuBisCO and carbonic anhydrase on a DNA nanoscaffold (2015)
  • Author(s): Huyen Dinh, Eiji Nakata, Tien Anh Ngo, Hiroki Ashida, Takashi Morii
  • Organizer: ISNAC2015
  • Place of Presentation: 兵庫
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] Construction of DNA nanotubes toward the three-dimensionally constrained assembly of enzymes (2015)
  • Author(s): Keisuke Nishida, Huyen Dinh, Eiji Nakata, Takashi Morii
  • Organizer: ISNAC2015
  • Place of Presentation: 兵庫
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] Expansion of modular adaptors for covalently locating multiple enzymes on DNA nanoscaffold (2015)
  • Author(s): Thang Minh Nguyen, Eiji Nakata, Masayuki Saimura, Takashi Morii
  • Organizer: ISNAC2015
  • Place of Presentation: 兵庫
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] Site-specific covalent modification of individual protein molecule on (2015)
  • Author(s): Eiji Nakata, Huyen Dinh, Nguyen Minh Thang, Ngo Tien Anh,
  • Organizer: ISNAC2015
  • Place of Presentation: 兵庫
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] DNAナノ構造体への共有結合型DNA結合アダプターを介した酵素の配置 (2015)
  • Author(s): 中田 栄司 ・ Huyen Dinh ・ Nguyen Minh Thang ・ Ngo Tien Anh ・ 才村 正幸 ・ 森井 孝
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2015-09-11
• [Presentation] 三次元的な酵素配置を目指したDNA ナノチューブの構築 (2015)
  • Author(s): 西田 圭佑 ・ Dinh Huyen ・ 中田 栄司 ・ 森井 孝
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2015-09-11
• [Presentation] Orthogonal assembly of RuBisCO and Carbonic Anhydrase on a DNA nanoscaffold (2015)
  • Author(s): Dinh Huyen ・ Nakata Eiji ・ Ngo Anh Tien ・ Ashida Hiroki ・ Morii Takashi
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2015-09-10
• [Remarks]
  • URL: http://www.iae.kyoto-u.ac.jp/material/index.html
• [Remarks]
  • URL: https://pubs.acs.org/page/jacsat/vi/engineered-bio-catalysts?utm_source=JACS&utm_medium=IMG&utm_campaign=PUBS1017EJK
• [Remarks]
  • URL: http://www.iae.kyoto-u.ac.jp/new-iae/NewsRelease/JP/2017/06/01-162221.html
• [Remarks]
  • URL: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/170518_2.html
• [Remarks]
  • URL: http://www.iae.kyoto-u.ac.jp/material/index.html
• [Remarks]
  • URL: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/170518_2.html
• [Remarks]
  • URL: http://www.iae.kyoto-u.ac.jp/new-iae/NewsRelease/JP/2017/06/01-162221.html
• [Remarks]
  • URL: https://academist-cf.com/journal/?p=5281