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Creation of spatially and temporally resolved flow measurement method for nanochannels utilizing single molecule as tracer

Research Project

Project/Area Number 15H05507
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Fluid engineering
Research InstitutionKeio University (2018-2019)
The University of Tokyo (2015-2017)

Principal Investigator

Kazoe Yutaka  慶應義塾大学, 理工学部(矢上), 講師 (20600919)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords流体計測 / ナノチャネル / 単一分子 / エバネッセント波
Outline of Final Research Achievements

Microfluidics has rapidly developed to miniaturize and integrate various fluidic operations. Recently, it has been further downscaled to nanofluidics. In this study, in order to understand fluid flows in ultrasmall nanospaces, we proposed a measurement method of flow velocity distribution by tracing the motion of single-molecules in fluid flows, which we call molecular image velocimetry (MIV). A tracer molecule with controlled size was developed by fluorescence modification of dendrimer, and applied to measurements of pressure-driven flow in a nanochannel. The proposed method and obtained knowledge will contribute to understand fluid dynamics and molecular transport in nanospaces.

Academic Significance and Societal Importance of the Research Achievements

分析装置や医療診断機器、リアクター等の様々な流体システムの小型化・デバイス化が進み、微小空間の流体(液体、気体)の流れの理解が重要になっている。これまでに10μmのマイクロ空間の流れの研究が進んできたが、ナノ空間は極めて小さく研究が難しかった。本研究は、極小のナノ空間の流れを計測する新たな方法論を提案し、この空間の流体力学と移動現象論の確立に寄与するものである。また、生物物理における細胞間・細胞内ナノ空間の分子輸送、膜工学における物質分離など他分野で扱うナノ空間の現象解明に有用な知見を提供するものである。

Report

(5 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (46 results)

All 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (10 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (32 results) (of which Int'l Joint Research: 13 results,  Invited: 10 results) Remarks (3 results)

  • [Int'l Joint Research] National Kaohsiung Normal University(台湾)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Time resolution effect on the apparent particle dynamics confined in a nanochannel evaluated by the single particle tracking subject to Brownian motion2018

    • Author(s)
      Itsuo Hanasaki, Yutaka Kazoe, Takehiko Kitamori
    • Journal Title

      Microfluidics and Nanofluidics

      Volume: 22 Issue: 5 Pages: 56-56

    • DOI

      10.1007/s10404-018-2073-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pressure-driven injection of charged solute molecules from micro to nanochannel2018

    • Author(s)
      Kazuma Okamoto, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori
    • Journal Title

      Proceedings of MicroTAS2018

      Volume: - Pages: 438-439

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Defocusing nano particle image velocimetry for nanochannel flows2017

    • Author(s)
      Kazuki Shibata, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori
    • Journal Title

      Proceedings of MicroTAS2017

      Volume: - Pages: 261-262

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of fluid flows in 10^1 nm extended-nano space2016

    • Author(s)
      Yutaka Kazoe, Keisuke Ikeda, Kyojiro Morikawa, Yuriy Pihosh, Kazuma Mawatari, Takehiko Kitamori
    • Journal Title

      Proceedings of MicroTAS2016

      Volume: - Pages: 798-799

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of flow velocity distribution in extended-nano spaces by molecular image velocimetry2016

    • Author(s)
      Shigenori Takeda, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori
    • Journal Title

      Proceedings of MicroTAS2016

      Volume: - Pages: 798-799

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-pressure acceleration of nanoliter droplets in the gas phase in a microchannel2016

    • Author(s)
      Yutaka Kazoe, Ippei Yamashiro, Kazuma Mawatari, Takehiko Kitamori
    • Journal Title

      Micromachines

      Volume: 7 Issue: 8 Pages: 142-142

    • DOI

      10.3390/mi7080142

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular image velocimetry for measuring flow velocity distribution in extended-nanochannel2015

    • Author(s)
      Yutaka Kazoe, Yojiro Hiramatsu, Kazuma Mawatari, Takehiko Kitamori
    • Journal Title

      Proceedings of MicroTAS2015

      Volume: - Pages: 1127-1129

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Formation of parallel aq/org two-phase flows in extended-nanochannel by partial hydrophobic modification2015

    • Author(s)
      Yutaka Kazoe, Takuya Ugajin, Ryoichi Ohta, Kazuma Mawatari, Takehiko Kitamori
    • Journal Title

      Proceedings of MicroTAS2015

      Volume: - Pages: 1029-1031

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 流体流路の階層サイズ構造とバイオデバイスへの応用2015

    • Author(s)
      北森武彦、馬渡和真、嘉副裕
    • Journal Title

      応用物理

      Volume: 84 Pages: 882-888

    • NAID

      130007718665

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 拡張ナノ空間の流体科学に関する研究2015

    • Author(s)
      嘉副裕
    • Journal Title

      化学とマイクロ・ナノシステム学会誌

      Volume: 14 Pages: 10-10

    • Related Report
      2015 Annual Research Report
  • [Presentation] マイクロ・ナノ空間の高度流体制御技術とデバイス化2019

    • Author(s)
      嘉副裕
    • Organizer
      第3回東工大応用化学系次世代を担う若手シンポジウム、東京工業大学、東京
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Pioneering nanofluidics2019

    • Author(s)
      北森武彦, 嘉副裕
    • Organizer
      4大学ナノ・マイクロファブリケーションコンソーシアム10周年記念シンポジウム, 新川崎・創造のもり地区AIRBIC大会議室, 川崎
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] マイクロ/拡張ナノサイズインターフェースにおける溶質分子挙動2019

    • Author(s)
      岡本一真, 嘉副裕, 馬渡和真, 北森武彦
    • Organizer
      日本化学会第99春季年会, 甲南大学, 兵庫
    • Related Report
      2018 Annual Research Report
  • [Presentation] Pressure-driven injection of charged solute molecules from micro to nanochannel2018

    • Author(s)
      Kazuma Okamoto, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2018), Kaohsiung, Taiwan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ/拡張ナノサイズインターフェースにおける溶質分子挙動の支配因子2018

    • Author(s)
      岡本一真, 嘉副裕, 馬渡和真, 北森武彦
    • Organizer
      化学とマイクロ・ナノシステム学会第38回研究会, 札幌市民交流プラザ, 北海道
    • Related Report
      2018 Annual Research Report
  • [Presentation] TIRFによるナノ流路内のトレーサー可視化における露光時間の影響2018

    • Author(s)
      花崎逸雄, 嘉副裕, 北森武彦
    • Organizer
      第46回可視化情報シンポジウム, 明治大学, 東京
    • Related Report
      2018 Annual Research Report
  • [Presentation] デフォーカスナノ粒子像を利用したナノ流路の流速分布計測法の開発2018

    • Author(s)
      嘉副裕, 柴田和輝, 馬渡和真, 北森武彦
    • Organizer
      第46回可視化情報シンポジウム, 明治大学, 東京
    • Related Report
      2018 Annual Research Report
  • [Presentation] Effect of electrostatic interaction on solute molecules behavior at sampling into nanochannel2018

    • Author(s)
      Kazuma Okamoto, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      The 10th International Symposium on Microchemistry and Microsystems (ISMM2018), Busan, Korea
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 拡張ナノ空間流速分布測定に向けたデフォーカス粒子像の解析2018

    • Author(s)
      柴田和輝, 嘉副裕, 馬渡和真, 北森武彦
    • Organizer
      化学とマイクロ・ナノシステム学会第37回研究会, 産業技術総合研究所, 茨城
    • Related Report
      2018 Annual Research Report
  • [Presentation] Nanoscale fluid flows in nanofluidic devices2017

    • Author(s)
      Yutaka Kazoe, Hiroki Sano, Takuya Ugajin, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      FFSCI-Nano Science/EMN Croatia Meeting
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Study on aqueous/organic two-phase flow in nanochannel for femto-liter solvent extraction2017

    • Author(s)
      Yutaka Kazoe, Hiroki Sano, Takuya Ugajin, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      Implementation of Microreactor Technology in Biotechnology 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of measurement method of fluid velocity distribution in nanochannels utilizing defocused particle image2017

    • Author(s)
      Yutaka Kazoe, Kazuki Shibata, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      Microfluidic Compartmentalization Workshop (OIST) "Fundamentals and Applications of Microfluidic Compartmentalization"
    • Related Report
      2017 Annual Research Report
  • [Presentation] 拡張ナノ空間の表面化学基と流体特性2016

    • Author(s)
      池田啓輔、嘉副裕、森川響二朗、塚原剛彦、馬渡和真、北森武彦
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学、京都
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Unique liquid properties by surface silanol groups in extended-nano spaces2016

    • Author(s)
      Yutaka Kazoe, Keisuke Ikeda, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      PITTCON Conference & Expo 2016
    • Place of Presentation
      Atlanta, USA
    • Year and Date
      2016-03-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] エバネッセント波を利用した拡張ナノ空間の流速分布計測2016

    • Author(s)
      嘉副裕
    • Organizer
      第30回先端光量子科学アライアンスセミナー「ナノ・マイクロファブリケーションを活用した光科学の展開」シンポジウム
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] マイクロ・ナノ流体工学の進展と新機能デバイスへの応用2016

    • Author(s)
      嘉副裕
    • Organizer
      先進システムデザイン工学
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Study on fluid flows in 10^1 nm extended-nano space2016

    • Author(s)
      Keisuke Ikeda, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      The 8th International Symposium on Microchemistry and Microsystems (ISMM2016)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] デンドリマー蛍光修飾法の開発と拡張ナノ空間流速分布測定への応用2016

    • Author(s)
      竹田薫識、嘉副裕、馬渡和真、北森武彦
    • Organizer
      化学とマイクロ・ナノシステム学会第33回研究会
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] エバネッセント波を利用した拡張ナノ空間の流速分布計測2016

    • Author(s)
      嘉副裕, 馬渡和真, 北森武彦
    • Organizer
      第30回先端光量子科学アライアンスセミナー「ナノ・マイクロファブリケーションを活用した光科学の展開」シンポジウム
    • Place of Presentation
      慶應義塾大学, 横浜
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] NMR study on neurotransmitter diffusion in synaptic cleft using biomimetic extended-nanospaces2015

    • Author(s)
      Yutaka Kazoe, Yoshitaka Tsuchiya, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unique properties of water induced by surface chemical groups in extended nanospace2015

    • Author(s)
      Keisuke Ikeda, Yutaka Kazoe, Takehiko Tsukahara, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 拡張ナノ空間の表面-溶媒相互作用と流体特性2015

    • Author(s)
      池田啓輔、嘉副裕、塚原剛彦、馬渡和真、北森武彦
    • Organizer
      化学とマイクロ・ナノシステム学会第32回研究会
    • Place of Presentation
      北九州国際会議場、北九州
    • Year and Date
      2015-11-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Formation of parallel two-phase flow in nanochannel and application to solvent extraction2015

    • Author(s)
      Yutaka Kazoe, Takuya Ugajin, Ryoichi Ohta, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      68th Annual Meeting of the APS Division of Fluid Dynamics
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of liquid properties in extended nanospaces using streaming potential/current system2015

    • Author(s)
      Kyojiro Morikawa, Yutaka Kazoe, Chih-Chang Chang, Takehiko Tsukahara, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      68th Annual Meeting of the APS Division of Fluid Dynamics
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Formation of parallel aq./org. two-phase flow in extended-nano channel for fL chemical analysis2015

    • Author(s)
      Yutaka Kazoe, Takehiko Kitamori
    • Organizer
      IEEE International Conference on Nano/Molecular Medicine & Engineering 2015
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2015-11-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fluid science in extended-nano space for novel chemical devices2015

    • Author(s)
      Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      The International Conference on Small Science
    • Place of Presentation
      Phuket, Thailand
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 拡張ナノ流路内疎水部分修飾による平行二相流の形成2015

    • Author(s)
      嘉副裕、宇賀神拓也、馬渡和真、北森武彦
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学, 福岡
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Surface-solvent interaction and fluid properties in extended nanospace2015

    • Author(s)
      Keisuke Ikeda, Yutaka Kazoe, Takehiko Tsukahara, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      RSC Tokyo International Conference 2015-Analytical Technology Towards Life Innovation
    • Place of Presentation
      Makuhari Messe, Chiba
    • Year and Date
      2015-09-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Parallel aq./org. two-phase flow in extended-nanochannel formed by partial hydrophobic modification2015

    • Author(s)
      Yutaka Kazoe, Takuya Ugajin, Ryoichi Ohta, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      The 22nd International Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techniques (ITP2015)
    • Place of Presentation
      Helsinki, Finland
    • Year and Date
      2015-08-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Formation of parallel two-phase flow in extended-nanochannel by partial hydrophobic modification2015

    • Author(s)
      Yutaka Kazoe, Takuya Ugajin, Ryoichi Ohta, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      The 7th International Symposium on Microchemistry and Microsystems (ISMM2015)
    • Place of Presentation
      Kyoto University, Kyoto
    • Year and Date
      2015-06-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 拡張ナノ空間の流体科学に関する研究2015

    • Author(s)
      嘉副裕
    • Organizer
      化学とマイクロ・ナノシステム学会第31回研究会
    • Place of Presentation
      京都大学, 京都
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] さらに小さく!10-6と10-9の間・・・拡張ナノ化学2015

    • Author(s)
      嘉副裕
    • Organizer
      先進環境材料・デバイス創製スクール「手のひらに化学工場-マイクロ化学チップコース-」
    • Place of Presentation
      新川崎・創造のもり, 横浜
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Remarks] 熱流体工学研究室

    • URL

      http://www.tfe.sd.keio.ac.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 北森研究室

    • URL

      http://webpark1390.sakura.ne.jp/cms/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 北森研究室ホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/kitamori/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report 2015 Annual Research Report

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Published: 2015-04-16   Modified: 2022-11-04  

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