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Virtual molecular display by nanoscience and mechatronics for nanobiotechnology

Research Project

Project/Area Number 15H05511
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionHirosaki University (2017-2019)
The University of Tokyo (2015-2016)

Principal Investigator

Hoshino Takayuki  弘前大学, 理工学研究科, 准教授 (00516049)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywordsメカトロニクス / ナノバイオテクノロジー / 分子機械 / バーチャル電極ディスプレイ / 微小管 / 局所電場 / オペランド計測 / 電子線描画 / バーチャル電極 / 電気化学 / 分子モータ / 界面化学 / 走査型電気化学顕微鏡 / 人工脂質膜 / 動電現象 / 脂質分子 / ナノロボティクス / 電子線 / ナノバイオ / バーチャル・カソードディスプレイ / 窒化シリコン薄膜 / DNA / 界面動電現象 / 細胞内ひずみ分布 / インターカレーション / 表面・界面物性 / マイクロ・ナノデバイス / 生物・生体工学 / 細胞・組織 / 倒立電子顕微鏡 / ナノポア / エレクトロポレーション / ナノマイクロメカトロニクス / 分子間相互作用 / バーチャル・リアリティ
Outline of Final Research Achievements

This research proposed nanobio manipulation method based on the control of Coulomb potential and ion concentration in biological environment. Spatial charge density control by electron beam drawing is a fundamental technology to control the spatial and temporal charge density of biomolecular systems in this research. Our research aims to understand the system of mechanical functions with biomolecular interactions which can be manipulated by potential and ion concentration as desired. This method, which directly manipulates intermolecular interactions, is also applied to the induction of self-assembly phenomena, which is important for the construction of next-generation nanostructured materials.

Academic Significance and Societal Importance of the Research Achievements

バイオ環境中の生体分子に界面動電現象や電気化学現象を用いて操作することで,分子の時空間パターニングという基礎課程だけでなく,細胞接着分子の結合状態を操作して細胞内に働いている弾性ひずみエネルギー分布を可視化することや,これまで見ることができなかった接着界面の電気化学的イメージングに応用できることを示すなど,本研究で取り組んできたバーチャル電極を自在に提示,操作する技術は細胞工学やナノバイオテクノロジーなど新しい技術革新が求められている分野に貢献するものである.

Report

(5 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (44 results)

All 2020 2019 2018 2017 2016 2015 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 1 results,  Acknowledgement Compliant: 6 results) Presentation (32 results) (of which Int'l Joint Research: 12 results) Remarks (1 results)

  • [Journal Article] Pause of the target gliding microtuble on the virtual cathode2019

    • Author(s)
      Hatazawa Kenta、Miyazako Hiroki、Kawamura Ryuzo、Hoshino Takayuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 514 Issue: 3 Pages: 821-825

    • DOI

      10.1016/j.bbrc.2019.04.200

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Engineered topographical structure to control spatial cell density using cell migration2019

    • Author(s)
      Okutani Chihiro、Wagatsuma Akira、Mabuchi Kunihiko、Hoshino Takayuki
    • Journal Title

      Biomedical Microdevices

      Volume: 21 Issue: 4 Pages: 98-98

    • DOI

      10.1007/s10544-019-0447-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pinpoint Delivery of Molecules by Using Electron Beam Addressing Virtual Cathode Display2018

    • Author(s)
      Hoshino Takayuki、Yoshioka Moto、Wagatsuma Akira、Miyazako Hiroki、Mabuchi Kunihiko
    • Journal Title

      IEEE Transactions on NanoBioscience

      Volume: 17 Issue: 1 Pages: 62-69

    • DOI

      10.1109/tnb.2018.2798582

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temporal relation between neural activity and neurite pruning on a numerical model and a microchannel device with micro electrode array2017

    • Author(s)
      Yohei Kondo, Yuichiro Yada, Tatsuya Haga, Yuzo Takayama, Takuya Isomura, Yasuhiko Jimbo, Osamu Fukayama, Takayuki Hoshino, Kunihiko Mabuchi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 486 Issue: 2 Pages: 539-544

    • DOI

      10.1016/j.bbrc.2017.03.082

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell step down by boundary curvature of high topographical structure for the device changing cell density2017

    • Author(s)
      Chihiro Okutani, Akira Wagatsuma, Kunihiko Mabuchi, and Takayuki Hoshino
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 56

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell2016

    • Author(s)
      Takayuki Hoshino, Hiroki Miyazako, Atsuki Nakayama, Akira Wagatsuma, and Kunihiko Mabuchi
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 236 Pages: 659-667

    • DOI

      10.1016/j.snb.2016.06.023

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In situ patterning of organic molecules in aqueous solutions using an inverted electron-beam lithography system2016

    • Author(s)
      Hiroki Miyazako, Kazuhiko Ishihara, Kunihiko Mabuchi, and Takayuki Hoshino
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 55 Issue: 6S1 Pages: 06GL07-06GL07

    • DOI

      10.7567/jjap.55.06gl07

    • NAID

      210000146670

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In-situ Patterning of Organic Molecules in Aqueous Solutions Using an Inverted Electron-beam Lithography System2016

    • Author(s)
      Hiroki Miyazako, Kazuhiko Ishihara , Kunihiko Mabuchi, and Takayuki Hoshino
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 55

    • NAID

      210000146670

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of ageing on expression of the muscle-specific E3 ubiquitin ligases and Akt-dependent regulation of Foxo transcription factors in skeletal muscle2016

    • Author(s)
      Akira Wagatsuma, Masataka Shiozuka, Yuzo Takayama, Takayuki Hoshino, Kunihiko Mabuchi, and Ryoichi Matsuda
    • Journal Title

      Molecular and Cellular Biochemistry

      Volume: 412 Issue: 1-2 Pages: 59-72

    • DOI

      10.1007/s11010-015-2608-7

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electron-beam-controlled reversible concentration of nanoparticles on a nanopore2015

    • Author(s)
      Takayuki Hoshino, and Kunihiko Mabuchi
    • Journal Title

      Applied Physics Express

      Volume: 8 Issue: 8 Pages: 087001-087001

    • DOI

      10.7567/apex.8.087001

    • NAID

      210000137629

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Spatiotemporal Control of Electrokinetic Transport in Nanofluidics Using an Inverted Electron-Beam Lithography System2015

    • Author(s)
      Hiroki Miyazako, Kunihiko Mabuchi, and Takayuki Hoshino
    • Journal Title

      Langmuir

      Volume: 31 Issue: 23 Pages: 6595-6603

    • DOI

      10.1021/acs.langmuir.5b00806

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 微小管滑走に対する電場の時空間的変化の影響2020

    • Author(s)
      畑澤 研太, 川村 隆三, and 星野 隆行
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 微小管再滑走時の方向変化に対する座屈の影響2020

    • Author(s)
      中村正啓, 畑澤研太, 川村隆三, and 星野隆行
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Detachment of the Biomolecule Bindings between the Microtubules and Kinesins2019

    • Author(s)
      Kenta Hatazawa, Ryuzo Kawamura, Masahiro Nakamura, and Takayuki Hoshino
    • Organizer
      2019 MRS Fall Meeting & Exhibit
    • Related Report
      2018 Annual Research Report
  • [Presentation] 電子線バーチャル電極ディスプレイによる支持脂質二重 膜の流動ダイナミクスの時空間制御,2018

    • Author(s)
      宮廻 裕樹,星野 隆行
    • Organizer
      第65回応用物理学会春季学術講演会, 早稲田大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電子線走査によるバーチャル電極ディスプレイを用いたウェットサンプルの接着界面イメージング2018

    • Author(s)
      遠山 渉, 宮廻 裕樹, 星野 隆行
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電子線励起バーチャル電極による液体試料の電気化学イメージング,2017

    • Author(s)
      宮廻裕樹, 満渕邦彦, 星野隆行
    • Organizer
      第64回応用物理学会春季講演会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 電子線励起バーチャル電極による液体試料の電気化学イメージング2017

    • Author(s)
      遠山渉, 宮廻裕樹, 満渕邦彦, 星野隆行
    • Organizer
      第64回応用物理学会春季講演会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 地形的構造による細胞走性の誘導を用いた細胞密度変化2017

    • Author(s)
      奥谷智裕, 我妻玲, 満渕邦彦, 星野隆行
    • Organizer
      第64回応用物理学会春季講演会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] バーチャル電極ディスプレイの単一細胞解析への応用-細胞内ひずみ解析とピンポイントエレクトロポレーション-2017

    • Author(s)
      星野隆行, 宮廻裕樹, 吉岡基, 我妻玲, 満渕邦彦
    • Organizer
      第64回応用物理学会春季講演会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 筋芽細胞のネマチック性に基づく細胞配向パターンの誘導のための境界設計法2017

    • Author(s)
      富井潤,宮廻裕樹,奥谷智裕,我妻玲,星野隆行,満渕邦彦
    • Organizer
      計測自動制御学会第29回自律分散システム・シンポジウム
    • Place of Presentation
      調布クレストンホテル(東京)
    • Year and Date
      2017-01-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] FINE VIRTUAL CATHODE DISPLAY FOR BIOMOLECULES CONTROL AND CELL NANO SURGERY2017

    • Author(s)
      Takayuki Hoshino, Moto Yoshioka, Akira Wagatsuma, Hiroki Miyazako. and Kunihiko Mabuchi
    • Organizer
      The 30th IEEE International Conference on Micro Electro Mechanical Systems (MEMS2017)
    • Place of Presentation
      Las Vegas (USA)
    • Year and Date
      2017-01-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Directed Cell Migration in Co-Cultures by Topographic Curvature for Heterogeneous Tissue Engineering2017

    • Author(s)
      Chihiro Okutani, Akira Wagatsuma, Kunihiko Mabuchi, and Takayuki Hoshino
    • Organizer
      The 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamic Electromechanical Control of Biomolecules using a Nano Virtual Cathode Display2017

    • Author(s)
      Hiroki Miyazako, Kunihiko Mabuchi, and Takayuki Hoshino
    • Organizer
      The 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電子線による高精細バーチャル電極ディスプレイを用いた膜ドメインと膜形態の動的制御(Dynamic Control of Membrane Domains and Morphology Using an Electron-beam Induced Fine Virtual Cathode Display)2017

    • Author(s)
      宮廻裕樹, 星野隆行
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電子線によるバーチャル電極を用いた人工脂質膜の動的パターニング2017

    • Author(s)
      宮廻裕樹, 星野隆行
    • Organizer
      化学とマイクロ・ナノシステム学会第36回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電子線走査型バーチャル電極によるナノ界面の電気化学イメージング2017

    • Author(s)
      遠山渉, 宮廻裕樹, 星野隆行
    • Organizer
      化学とマイクロ・ナノシステム学会第36回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Electrical Control for Self-Assembly of Biomaterials Using an Electron-Beam-Induced Virtual Electrode2016

    • Author(s)
      Hiroki Miyazako, Kunihiko Mabuchi, and Takayuki Hoshino
    • Organizer
      2016 MRS Fall Meeting & Exhibit
    • Place of Presentation
      Boston, USA
    • Year and Date
      2016-11-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] VIRTUAL ELECTRODE FOR MECHANICAL STRAIN MICROSCOPY AND ELECTROPORATION ON INVERTED-ELECTRON BEAM LITHOGRPHY2016

    • Author(s)
      Takayuki Hoshino, Moto Yoshioka, Hiroki Miyazako, Akira Wagatsuma, Kunihiko Mabuchi
    • Organizer
      International Conference on Single Cell Research 2016
    • Place of Presentation
      UTokyo, Tokyo (Japan)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] TOPOGRAPHICAL STRUCTURE INDUCED FUNCTIONAL SINGLE CELL MIGRATION: A HOLE-SIDE WALL TRAPPING CELL AND ENHANCING CELL MIGRATION SPEED2016

    • Author(s)
      Chihiro Okutani, Akira Wagatsuma, Hiroki Miyazako, Kunihiko Mabuchi, Takayuki Hoshino
    • Organizer
      International Conference on Single Cell Research 2016
    • Place of Presentation
      UTokyo, Tokyo (Japan)
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cell Migration Control by Boundary Shape of Topographical Structure2016

    • Author(s)
      Chihiro Okutani, Akira Wagatsuma, Kunihiko Mabuchi, and Takayuki Hoshino
    • Organizer
      The 29th International Microprocesses and Nanotechnology Conference (MNC2016)
    • Place of Presentation
      ANA Crown Plasza Hotel Kyoto, Kyoto (Japan)
    • Year and Date
      2016-11-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fluorescence Over-Recovery in Transient Response of Bacteriophage T4 DNA with YOYO-1 after Electrical Stimulation on Virtual Electrode2016

    • Author(s)
      Takayuki Hoshino, and Kunihiko Mabuchi
    • Organizer
      The 29th International Microprocesses and Nanotechnology Conference (MNC2016)
    • Place of Presentation
      ANA Crown Plasza Hotel Kyoto, Kyoto (Japan)
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-situ Manipulation of Giant Liposomes Using an Electron-beam Induced Virtual Cathode2016

    • Author(s)
      Hiroki Miyazako, Kunihiko Mabuchi, Takayuki Hoshino
    • Organizer
      RSC Tokyo International Conference 2016
    • Place of Presentation
      Makuhari Messe, Chiba(Japan)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電子線による局所電場を用いたリポソームの形状操作2016

    • Author(s)
      宮廻裕樹,満渕邦彦, 星野隆行
    • Organizer
      化学とマイクロ・ナノシステム学会第34回研究会
    • Place of Presentation
      幕張メッセ(千葉)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 電子線による局所電場印加システムを用いた高分子レオロジーのその場制御2016

    • Author(s)
      宮廻裕樹,満渕邦彦,星野隆行
    • Organizer
      化学とマイクロ・ナノシステム学会第33回研究会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2016-04-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 電子線誘起局所電界操作によるバイオ分子操作とin-situリソグラフィ2016

    • Author(s)
      星野隆行, 宮廻裕樹, 満渕邦彦
    • Organizer
      第63回応用物理学会春季講演会
    • Place of Presentation
      東京工業大学,東京,目黒区
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 地形的なパターンによる筋芽細胞C2C12の運動への影響2016

    • Author(s)
      奥谷智裕, 我妻玲, 米谷玲皇, 満渕邦彦, 星野隆行
    • Organizer
      63回応用物理学会春季講演会
    • Place of Presentation
      東京工業大学,東京,目黒区
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 倒立型電子線描画を用いた細胞膜電気穿孔現象2016

    • Author(s)
      吉岡基, 宮廻裕樹, 我妻玲, 満淵邦彦, 星野隆行
    • Organizer
      第63回応用物理学会春季講演会
    • Place of Presentation
      東京工業大学,東京都目黒区
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 2D control of ionic transport and electrochemical reaction in nanofluidics using a highly focused electric field of an electron-beam2015

    • Author(s)
      Hiroki Miyazako, Kunihiko Mabuchi ,and Takayuki Hoshino
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem))
    • Place of Presentation
      Marriot, Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Temporal Control of Bacteriophage T4 DNA Concentration on a Nanopore by Using Inverted-Electron Beam Lithography2015

    • Author(s)
      Takayuki Hoshino, and Kunihiko Mabuchi
    • Organizer
      The 28th International Microprocesses and Nanotechnology Conference (MNC2015)
    • Place of Presentation
      Toyama International Conference Center, Toyama,Japan
    • Year and Date
      2015-10-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamic Patterning of Organic Molecules in Aqueous Solutions Using an Inverted Electron-Beam Lithography System2015

    • Author(s)
      Hiroki Miyazako, Kazuhiko Ishihara, Kunihiko Mabuchi, and Takayuki Hoshino
    • Organizer
      The 28th International Microprocesses and Nanotechnology Conference (MNC2015)
    • Place of Presentation
      Toyama International Conference Center, Toyama, Japan
    • Year and Date
      2015-10-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電子線の動電現象による単一接着性細胞への局所的な染色液導入の観察 (Observation of Local Dye Inflow into Single Adherent Cells induced by Electrokinetic Phenomena of Electron Beam)2015

    • Author(s)
      吉岡基,宮廻裕樹,我妻玲,満渕邦彦,星野隆行
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学,石川県金沢市
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生化学分析のための電子線による有機カチオン輸送制御 (Transportation Control of Organic Cations Using an Electron-beam for Biochemical Analysis)2015

    • Author(s)
      宮廻裕樹, 藤田恭子,中村暢文, 大野弘幸, 満渕邦彦,星野隆行
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学,石川県金沢市
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Remarks] 弘前大学 星野研究室

    • URL

      https://sites.google.com/view/hoshino-lab/researches/display

    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2022-11-04  

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