In vivo imaging of synaptic plasticity

• Project/Area Number: 15H05571
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Niigata University
• Principal Investigator: Matsui Hideaki 新潟大学, 研究推進機構, 准教授 (60710853)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
• Keywords: シナプス可塑性 / シナプス / 可視化 / シナプス機能 / 神経科学

Outline of Final Research Achievements

In this study, we aimed to visualize AMPA receptor specifically at the post synaptic membrane and to observe and monitor the plastic and dynamic changes of synaptic functions. In primary neurons, our indicator successfully enables visualization of synaptic functions during chemical LTP or LTD. We have continued to utilize this indicator for hippocampal slice and zebrafish in vivo.

Research Products

• [Journal Article] An optimized method for counting dopaminergic neurons in zebrafish (2017)
  • Author(s): Matsui Hideaki、Sugie Atsushi
  • Journal Title: PLoS One Volume: 12 Issue: 9 Pages: e0184363-e0184363
  • DOI: 10.1371/journal.pone.0184363
• [Journal Article] Parkinson’s disease pathogenesis from the viewpoint of small fish models (2017)
  • Author(s): Matsui Hideaki、Takahashi Ryosuke
  • Journal Title: J Neural Transm Volume: 125 Issue: 1 Pages: 25-33
  • DOI: 10.1007/s00702-017-1772-1
• [Journal Article] Dopamine system, cerebellum, and nucleus ruber in fish and mammals (2017)
  • Author(s): Matsui Hideaki
  • Journal Title: Dev Growth Differ Volume: 59 Issue: 4 Pages: 219-227
  • DOI: 10.1111/dgd.12357
• [Journal Article] Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light. (2017)
  • Author(s): Sugie A, Möhl C, Hakeda-Suzuki S, Matsui H, Suzuki T, Tavosanis G.
  • Journal Title: Journal of Visual Experiments Volume: - Issue: 120
  • DOI: 10.3791/55176
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The Use of Fish Models to Study Human Neurological Disorders (2017)
  • Author(s): Hideaki Matsui
  • Journal Title: Neuroscience Research Volume: 16 Pages: 1-7
  • DOI: 10.1016/j.neures.2017.02.004
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 魚のドパミン神経とパーキンソン病 (2017)
  • Author(s): Hideaki Matsui
  • Organizer: 第6回生理研霊長研脳研合同シンポジウム
  • Place of Presentation: 新潟大学脳研究所統合脳機能研究センター
  • Year and Date: 2017-03-09
  • Invited
• [Presentation] 小型魚類で明らかにする神経病態 (2016)
  • Author(s): Hideaki Matsui
  • Organizer: 第46回新潟神経学夏期セミナー
  • Place of Presentation: 新潟大学脳研究所統合脳機能研究センター
  • Year and Date: 2016-07-28
  • Invited
• [Presentation] 小型魚類を用いた神経疾患研究 (2016)
  • Author(s): Hideaki Matsui
  • Organizer: 第57回神経学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2016-05-18
  • Invited
• [Presentation] Teleost fish models of Parkinson’s disease. (2016)
  • Author(s): Hideaki Matsui
  • Organizer: Symposium: Basal Ganglia in Health and Disease (Satellite Symposium of Neuroscience 2016)
  • Place of Presentation: PACIFICO Yokohama
  • Invited